Comparative Phenotypic and Agronomic Assessment of Transgenic Potato with 3R-Gene Stack with Complete Resistance to Late Blight Disease

Transgenic potato event Vic.172, expressing three naturally occurring resistance genes (R genes) conferring complete protection against late blight disease, was evaluated for resistance to late blight, phenotypic characterization, and agronomic performance in field conditions at three locations during three seasons in Uganda. These trials were conducted by comparison to the variety Victoria from which Vic.172 derives, using identical fungicide treatment, except when evaluating disease resistance. During all seasons, the transgenic event Vic.172 was confirmed to have complete resistance to late blight disease, whereas Victoria plants were completely dead by 60–80 days after planting. Tubers from Vic.172 were completely resistant to LB after artificial inoculation. The phenotypic characterization included observations of the characteristics and development of the stems, leaves, flowers, and tubers. Differences in phenotypic parameters between Vic.172 and Victoria were not statistically significant across locations and seasons. The agronomic performance observations covered sprouting, emergence, vigor, foliage growth, and yield. Differences in agronomic performance were not statistically significant except for marketable yield in one location under high productivity conditions. However, yield variation across locations and seasons was not statistically significant, but was influenced by the environment. Hence, the results of the comparative assessment of the phenotype and agronomic performance revealed that transgenic event Vic.172 did not present biologically significant differences in comparison to the variety Victoria it derives from.

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Phenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States

Plant Disease ◽

10.1094/pdis-07-12-0682-re ◽

2013 ◽

Vol 97 (7) ◽

pp. 873-881 ◽

Cited By ~ 48

Author(s):

G. Danies ◽

I. M. Small ◽

K. Myers ◽

R. Childers ◽

W. E. Fry

Keyword(s):

United States ◽

Late Blight ◽

Phytophthora Infestans ◽

The United States ◽

Phenotypic Characterization ◽

Phenotypic Traits ◽

Mating Types ◽

Late Blight Disease ◽

Blight Disease

Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduction, the populations of P. infestans in the United States are primarily reproducing asexually and, thus, show a simple genetic structure. The emergence of new clonal lineages of P. infestans (US-22, US-23, and US-24) responsible for the late blight epidemics in the northeastern region of the United States in the summers of 2009 and 2010 stimulated an investigation into phenotypic traits associated with these genotypes. Mating type, differences in sensitivity to mefenoxam, differences in pathogenicity on potato and tomato, and differences in rate of germination were studied for clonal lineages US-8, US-22, US-23, and US-24. Both A1 and A2 mating types were detected. Lineages US-22, US-23, and US-24 were generally sensitive to mefenoxam while US-8 was resistant. US-8 and US-24 were primarily pathogenic on potato while US-22 and US-23 were pathogenic on both potato and tomato. Indirect germination was favored at lower temperatures (5 and 10°C) whereas direct germination, though uncommon, was favored at higher temperatures (20 and 25°C). Sporangia of US-24 released zoospores more rapidly than did sporangia of US-22 and US-23. The association of characteristic phenotypic traits with genotype enables the prediction of phenotypic traits from rapid genotypic analyses for improved disease management.

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Analysis of late-blight disease resistance and freezing tolerance in transgenic potato plants expressing sense and antisense genes for an osmotin-like protein

Planta ◽

10.1007/bf00197588 ◽

1996 ◽

Vol 198 (1) ◽

Cited By ~ 69

Author(s):

Baolong Zhu ◽

TonyH.H. Chen ◽

PaulH. Li

Keyword(s):

Disease Resistance ◽

Late Blight ◽

Freezing Tolerance ◽

Transgenic Potato ◽

Potato Plants ◽

Transgenic Potato Plants ◽

Late Blight Disease ◽

Blight Disease ◽

Antisense Genes

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GENETIC STABILITY ANALYSIS OF RB GENE IN GENETICALLY MODIFIED POTATO LINES TOLERANT TO Phytophthora infestans

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v16n2.2015.p.51-58 ◽

2016 ◽

Vol 16 (2) ◽

pp. 51

Author(s):

Edy Listanto ◽

Eny Ida Riyanti ◽

Tri Joko Santoso ◽

Toto Hadiarto ◽

A. Dinar Ambarwati

Keyword(s):

Stability Analysis ◽

Late Blight ◽

Blot Analysis ◽

Copy Number ◽

Southern Blot Analysis ◽

Transgenic Potato ◽

Plant Genome ◽

Late Blight Disease ◽

Rb Gene ◽

Blight Disease

Development of potato cultivars with high levels of broad spectrum resistance is a key long-term management strategy against late blight disease caused by Phytophthora infestans. Six progeny lines of hybridization between transgenic potato Katahdin SP951 with non-transgenic Granola and Atlantic were selected based on agronomical characteristics and resistance to late blight disease. The study aimed to analyze the number of insertions and stability of inserted RB gene in the transgenic potato lines. The research was carried out through plant DNA extraction, southern blot analysis and polymerase chain reaction (PCR). Southern blot analysis was used to detect the number of inserts integrated into potato genome, while PCR analysis was used to detect stability of RB gene from generation to generation. The results showed that the progenies obtained from hybridization between Atlantic and transgenic Katahdin SP951 (lines No. 20 and 27) and between Granola and transgenic Katahdin SP951 (line No. 69) contained one copy number of RB gene, according to the probing of nptII. The result is similar to that of inserted RB gene found in the parental transgenic Katahdin SP951. The presence of RB gene in four different generations (G0, G1, G2 and G3) showed stable integration of the gene into the plant genome. The single copy number of RB gene will repress the occurrence of silencing gene expression. The stability analysis of RB gene can determine that the gene is still present in plant genome after several generations.

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GENETIC STABILITY ANALYSIS OF RB GENE IN GENETICALLY MODIFIED POTATO LINES TOLERANT TO Phytophthora infestans

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v16n2.2015.pp.51-58 ◽

2016 ◽

Vol 16 (2) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Edy Listanto ◽

Eny Ida Riyanti ◽

Tri Joko Santoso ◽

Toto Hadiarto ◽

A. Dinar Ambarwati

Keyword(s):

Stability Analysis ◽

Late Blight ◽

Blot Analysis ◽

Copy Number ◽

Southern Blot Analysis ◽

Transgenic Potato ◽

Plant Genome ◽

Late Blight Disease ◽

Rb Gene ◽

Blight Disease

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Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

Indian Phytopathology ◽

10.1007/s42360-021-00330-6 ◽

2021 ◽

Vol 74 (1) ◽

pp. 181-187

Author(s):

Mehi Lal ◽

Sorabh Chaudhary ◽

Sanjay Rawal ◽

Sanjeev Sharma ◽

Manoj Kumar ◽

...

Keyword(s):

Late Blight ◽

Phytophthora Infestans ◽

Late Blight Disease ◽

Blight Disease

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Two new defensive constituents from potato tubers (Solanum tuberosum)

Zeitschrift für Naturforschung B ◽

10.1515/znb-2016-0234 ◽

2017 ◽

Vol 72 (6) ◽

pp. 393-396

Author(s):

Liangyan Liu ◽

Jun Han ◽

Yong Shen

Keyword(s):

Solanum Tuberosum ◽

Late Blight ◽

Quantum Chemical ◽

Spectroscopic Analysis ◽

2D Nmr ◽

Moderate Activity ◽

Potato Tubers ◽

Mycelia Growth ◽

Late Blight Disease ◽

Blight Disease

AbstractTwo new defensive constituents, solatuberenol A (1) and 3-O-β-d-glucopyranosyl stigmasta-5(6),24(28)-diene (2), were isolated from the potato tubers (Solanum tuberosum) infected with late blight disease. Their structures were identified by extensive spectroscopic analysis, including HRMS, IR, UV, 1D/2D NMR, ECD and quantum chemical calculations. Compounds 1 and 2 showed moderate activity against Phytophthora infestans with mycelia-growth inhibition of 30.1% and 52.4%, respectively, at the concentration of 500 ppm.

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The Assessment of Potato Late Blight Disease in Western Amhara Region, Ethiopia

SSRN Electronic Journal ◽

10.2139/ssrn.3882375 ◽

2021 ◽

Author(s):

Gebremariam Asaye Emrie ◽

Merkuz Abera Admassu ◽

Adane Tesfaye Lema

Keyword(s):

Late Blight ◽

Potato Late Blight ◽

Amhara Region ◽

Late Blight Disease ◽

Blight Disease

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Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1808585115 ◽

2018 ◽

Vol 115 (33) ◽

pp. E7834-E7843 ◽

Cited By ~ 20

Author(s):

Qin He ◽

Shaista Naqvi ◽

Hazel McLellan ◽

Petra C. Boevink ◽

Nicolas Champouret ◽

...

Keyword(s):

Late Blight ◽

Plant Pathogens ◽

Dominant Negative ◽

Host Protein ◽

Host Susceptibility ◽

Dominant Negative Mutant ◽

Virus Induced Gene Silencing ◽

Pathogen Effector ◽

Late Blight Disease ◽

Blight Disease

Plant pathogens deliver effectors into plant cells to suppress immunity. Whereas many effectors inactivate positive immune regulators, other effectors associate with negative regulators of immunity: so-called susceptibility (S) factors. Little is known about how pathogens exploit S factors to suppress immunity. Phytophthora infestans RXLR effector Pi02860 interacts with host protein NRL1, which is an S factor whose activity suppresses INF1-triggered cell death (ICD) and is required for late blight disease. We show that NRL1 interacts in yeast and in planta with a guanine nucleotide exchange factor called SWAP70. SWAP70 associates with endosomes and is a positive regulator of immunity. Virus-induced gene silencing of SWAP70 in Nicotiana benthamiana enhances P. infestans colonization and compromises ICD. In contrast, transient overexpression of SWAP70 reduces P. infestans infection and accelerates ICD. Expression of Pi02860 and NRL1, singly or in combination, results in proteasome-mediated degradation of SWAP70. Degradation of SWAP70 is prevented by silencing NRL1, or by mutation of Pi02860 to abolish its interaction with NRL1. NRL1 is a BTB-domain protein predicted to form the substrate adaptor component of a CULLIN3 ubiquitin E3 ligase. A dimerization-deficient mutant, NRL1NQ, fails to interact with SWAP70 but maintains its interaction with Pi02860. NRL1NQ acts as a dominant-negative mutant, preventing SWAP70 degradation in the presence of effector Pi02860, and reducing P. infestans infection. Critically, Pi02860 enhances the association between NRL1 and SWAP70 to promote proteasome-mediated degradation of the latter and, thus, suppress immunity. Preventing degradation of SWAP70 represents a strategy to combat late blight disease.

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