scholarly journals Phenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 873-881 ◽  
Author(s):  
G. Danies ◽  
I. M. Small ◽  
K. Myers ◽  
R. Childers ◽  
W. E. Fry
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
The United States ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
Mating Types ◽  
Late Blight Disease ◽  
Blight Disease

Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduction, the populations of P. infestans in the United States are primarily reproducing asexually and, thus, show a simple genetic structure. The emergence of new clonal lineages of P. infestans (US-22, US-23, and US-24) responsible for the late blight epidemics in the northeastern region of the United States in the summers of 2009 and 2010 stimulated an investigation into phenotypic traits associated with these genotypes. Mating type, differences in sensitivity to mefenoxam, differences in pathogenicity on potato and tomato, and differences in rate of germination were studied for clonal lineages US-8, US-22, US-23, and US-24. Both A1 and A2 mating types were detected. Lineages US-22, US-23, and US-24 were generally sensitive to mefenoxam while US-8 was resistant. US-8 and US-24 were primarily pathogenic on potato while US-22 and US-23 were pathogenic on both potato and tomato. Indirect germination was favored at lower temperatures (5 and 10°C) whereas direct germination, though uncommon, was favored at higher temperatures (20 and 25°C). Sporangia of US-24 released zoospores more rapidly than did sporangia of US-22 and US-23. The association of characteristic phenotypic traits with genotype enables the prediction of phenotypic traits from rapid genotypic analyses for improved disease management.

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

Phylogenetic and phenotypic characterization of Fomes fasciatus and Fomes fomentarius in the United States

Mycologia ◽  
2013 ◽  
Vol 105 (6) ◽  
pp. 1524-1534 ◽  
Author(s):  
Meghan A. McCormick ◽  
Larry F. Grand ◽  
Justin B. Post ◽  
Marc A. Cubeta
Keyword(s):  
United States ◽  
The United States ◽  
Phenotypic Characterization ◽  
Fomes Fomentarius

scholarly journals First Report of Phytophthora infestans Genotype US23 Causing Late Blight in Canada

Plant Disease ◽  
2011 ◽  
Vol 95 (7) ◽  
pp. 873-873 ◽  
Author(s):  
L. M. Kawchuk ◽  
R. J. Howard ◽  
R. D. Peters ◽  
K. I. Al-Mughrabi
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
The United States ◽  
Western Canada ◽  
Eastern Canada ◽  
Disease Symptoms ◽  
Metalaxyl Sensitivity ◽  
Epidemiological Characteristics

Late blight is caused by the oomycete Phytophthora infestans (Mont.) de Bary and is one of the most devastating diseases of potato and tomato. Late blight occurs in all major potato- and tomato-growing regions of Canada. Its incidence in North America increased during 2009 and 2010 (2). Foliar disease symptoms appeared earlier than usual (June rather than July) and coincided with the identification of several new P. infestans genotypes in the United States, each with unique characteristics. Prior to 2007, isolates collected from potato and tomato crops were mainly US8 or US11 genotypes (1). However, P. infestans populations in the United States have recently experienced a major genetic evolution, producing isolates with unique genotypes and epidemiological characteristics in Florida and throughout the northeastern states (2). Recent discoveries of tomato transplants with late blight for sale at Canadian retail outlets prompted an examination of the genotypes inadvertently being distributed and causing disease in commercial production areas in Canada. Analysis of isolates of P. infestans from across Canada in 2010 identified the US23 genotype for the first time from each of the four western provinces (Manitoba, Saskatchewan, Alberta, and British Columbia) but not from eastern Canada. Allozyme banding patterns at the glucose phosphate isomerase (Gpi) locus indicated a 100/100 profile consistent with US6 and US23 genotypes (4). Mating type assays confirmed the isolates to be A1 and in vivo metalaxyl sensitivity was observed. Restriction fragment length polymorphic analysis of 50 isolates from western Canada with the multilocus RG57 sequence and EcoRI produced the DNA pattern 1, 2, 5, 6, 10, 13, 14, 17, 20, 21, 24, 24a, 25 that was indicative of US23 (3). The recently described P. infestans genotype US23 appears to be more aggressive on tomato, and although isolates were recovered from both tomato and potato, disease symptoms were often more severe on tomato. Results indicate that movement and evolution of new P. infestans genotypes have contributed to the increased incidence of late blight and that movement of the pathogen on retail plantlets nationally and internationally may provide an additional early season source of inoculum. A major concern is that the introduced new A1 populations in western Canada have established a dichotomy with the endogenous A2 populations in eastern Canada, increasing the potential for sexual recombination producing oospores and additional genotypes should these populations merge. References: (1) Q. Chen et al. Am. J. Potato Res. 80:9, 2003. (2) K. Deahl. (Abstr.) Phytopathology 100(suppl.):S161, 2010. (3) S. B. Goodwin et al. Curr. Genet. 22:107, 1992. (4) S. B. Goodwin et al. Phytopathology 88:939, 2004.

Field application of Bacillus subtilis isolates for controlling late blight disease of potato caused by Phytophthora infestans

2019 ◽  
Vol 22 ◽  
pp. 101366 ◽  
Author(s):  
Bhimanagoud Kumbar ◽  
Riaz Mahmood ◽  
S.N. Nagesha ◽  
M.S. Nagaraja ◽  
D.G. Prashant ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Field Application ◽  
Late Blight Disease ◽  
Blight Disease

Identification and Screening of Introduced Tomato Varieties Resistant to late Blight

2012 ◽  
Vol 610-613 ◽  
pp. 3472-3477
Author(s):  
Dan Luo ◽  
Xi Chun Zhang ◽  
Xiao Han Wen
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
New Disease ◽  
Tomato Production ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Detached Leaves ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Selection Of

Tomato late blight disease that infected by Phytophthora infestans (Mont.) de Bary has already become one of the main obstacles that influence the tomato production. To resolve tomato late blight disease, cultivating the disease-resistant breed is importance. Based on the previous study, different introduced varieties were used as materials in this test for the identification and varieties selection of tomato resistant to late blight, by measuring the DIS of inoculated seedlings and detached leaves. It indicated that Jingle 502 and O-33-1 are possible to become resistant varieties to late blight or the parents for cultivating new disease resistant varieties.

scholarly journals Genetic Change Within Populations of Phytophthora infestans in the United States and Canada During 1994 to 1996: Role of Migration and Recombination

1998 ◽  
Vol 88 (9) ◽  
pp. 939-949 ◽  
Author(s):  
Stephen B. Goodwin ◽  
Christine D. Smart ◽  
Robert W. Sandrock ◽  
Kenneth L. Deahl ◽  
Zamir K. Punja ◽  
...  
Keyword(s):  
United States ◽  
Sexual Reproduction ◽  
Phytophthora Infestans ◽  
High Sensitivity ◽  
The United States ◽  
Rapid Identification ◽  
Mating Types ◽  
Long Distance ◽  
New Genotype ◽  
The Us

Dramatic changes occurred within populations of Phytophthora infestans in the United States and Canada from 1994 through 1996. Occurrence of the US-8 genotype, detected rarely during 1992 and 1993, increased rapidly and predominated in most regions during 1994 through 1996. US-7, which infected both potato and tomato and made up almost 50% of the sample during 1993, was detected only rarely among 330 isolates from the United States analyzed during 1994. It was not detected at all in more limited samples from 1996. Thus, ability to infect both potato and tomato apparently did not increase the fitness of this genotype relative to US-8, as predicted previously. US-1, the previously dominant genotype throughout the United States and Canada, made up 8% or less of the samples analyzed during 1994 through 1996. A few additional genotypes were detected, which could indicate the beginnings of sexual reproduction of P. infestans within the United States and Canada. However, clonal reproduction still predominated in all locations sampled; opportunities for sexual reproduction probably were limited, because the A1 and A2 mating types usually were separated geographically. The high sensitivity of the US-1 genotype to the fungicide metalaxyl also could have reduced opportunities for contact between the mating types in fields where this compound was applied. The previous correlation between metalaxyl sensitivity and genotype was confirmed and extended to a new genotype, US-17: all US-1 isolates tested were sensitive; all isolates of the US-7, US-8, and US-17 genotypes tested to date have been resistant. Isolates of P. capsici and P. erythroseptica, two other species often found on tomato and potato, could be easily distinguished from each other and from P. infestans using a simple allozyme assay for the enzyme glucose-6-phosphate isomerase. This technique could be useful for rapid identification of species, in addition to genotype of P. infestans. It generally was not possible to predict which genotypes would be present in a location from 1 year to the next. Long-distance movement of US-8 in seed tubers was documented, and this was probably the primary means for the rapid spread of this genotype from 1993 through 1996.

Sign in / Sign up

Export Citation Format

Share Document