scholarly journals Sudden Cell Death Induced by Ca2+ Delivery via Microbubble Cavitation

Biomedicines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 32
Author(s):  
Martynas Maciulevičius ◽  
Diana Navickaitė ◽  
Sonam Chopra ◽  
Baltramiejus Jakštys ◽  
Saulius Šatkauskas
Keyword(s):  
Cell Death ◽  
Cell Membrane ◽  
Cell Viability ◽  
Metabolic Activity ◽  
Colony Formation ◽  
Calcium Concentration ◽  
Molecular Transport ◽  
Membrane Permeabilization ◽  
Inertial Cavitation ◽  
Cell Membrane Permeabilization

Intracellular calcium ion delivery via sonoporation has been validated to be a substitute for classical chemotherapy. However, the mechanism behind calcium sonoporation remains unclear to this day. To elucidate the role of calcium in the process of sonoporation, we aimed to investigate the influence of different calcium concentration on cell membrane permeabilization and cell viability after sonoporation. In this study, we present experimental evidence that extracellular calcium plays a major role in cell membrane molecular transport after applying ultrasound pulses. Ultrasound-microbubble cavitation in the presence of different calcium concentration affects fundamental cell bio-physio-chemical conditions: cell membrane integrity, metabolic activity, and colony formation. Corresponding vital characteristics were evaluated using three independent viability tests: propidium iodide assay (20 min–3 h), MTT assay (48 h), and cell clonogenic assay (6 d). The results indicate instant cell death, as the level of cell viability was determined to be similar within a 20 min–48 h–6 d period. Inertial cavitation activities have been determined to be directly involved in calcium delivery via sonoporation according to high correlation (R2 > 0.85, p < 0.01) of inertial cavitation dose with change in either cell membrane permeabilization, metabolic activity, and colony formation efficiency. In general, calcium delivery via sonoporation induces rapid cell death, occurring within 20 min after treatment, that is the result of ultrasound mediated microbubble cavitation.

Spatio-temporal dynamics of calcium electrotransfer during cell membrane permeabilization

2018 ◽  
Vol 8 (5) ◽  
pp. 1152-1161 ◽  
Author(s):  
Alexis Guionet ◽  
S. Moosavi Nejad ◽  
Justin Teissié ◽  
Takashi Sakugawa ◽  
Sunao Katsuki ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Temporal Dynamics ◽  
Membrane Permeabilization ◽  
Cell Membrane Permeabilization ◽  
Spatio Temporal

Characterization of plasma-induced cell membrane permeabilization: focus on OH radical distribution

2016 ◽  
Vol 49 (33) ◽  
pp. 334002 ◽  
Author(s):  
Shota Sasaki ◽  
Ryosuke Honda ◽  
Yutaro Hokari ◽  
Keisuke Takashima ◽  
Makoto Kanzaki ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeabilization ◽  
Oh Radical ◽  
Radical Distribution ◽  
Cell Membrane Permeabilization

scholarly journals Surfactant induces ROS-mediated cell membrane permeabilization for the enhancement of mannatide production

2020 ◽  
Vol 91 ◽  
pp. 172-180 ◽  
Author(s):  
Le Wang ◽  
Yu Sha ◽  
Dapeng Wu ◽  
Qixian Wei ◽  
Di Chen ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeabilization ◽  
Cell Membrane Permeabilization

Continuous-flow, electrically-triggered, single cell-level electroporation

TECHNOLOGY ◽  
2017 ◽  
Vol 05 (01) ◽  
pp. 31-41 ◽  
Author(s):  
Mingde Zheng ◽  
Joseph J. Sherba ◽  
Jerry W. Shan ◽  
Hao Lin ◽  
David I. Shreiber ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Field Strength ◽  
Electric Field ◽  
Cell Viability ◽  
Electric Field Strength ◽  
Continuous Flow ◽  
Intracellular Delivery ◽  
Membrane Permeabilization ◽  
Flow Environment ◽  
Cell Impedance

Electroporation creates transient openings in the cell membrane, allowing for intracellular delivery of diagnostic and therapeutic substances. The degree of cell membrane permeability during electroporation plays a key role in regulating the size of the delivery payload as well as the overall cell viability. A microfluidic platform offers the ability to electroporate single cells with impedance detection of membrane permeabilization in a high-throughput, continuous-flow manner. We have developed a flow-based electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. We are able to achieve the advantages of electrical monitoring of cell permeabilization, heretofore only achieved with trapped or static cells, while processing the cells in a continuous-flow environment. We demonstrate the analysis of membrane permeabilization on individual cells before and after electroporation in a continuous-flow environment, which dramatically increases throughput. We have confirmed cell membrane permeabilization by electrically measuring the changes in cell impedance from electroporation and by optically measuring the intracellular delivery of a fluorescent probe after systematically varying the electric field strength and duration and correlating the pulse parameters to cell viability. We find a dramatic change in cell impedance and propidium iodide (PI) uptake at a pulse strength threshold of 0.87 kV/cm applied for a duration of 1 ms or longer. The overall cell viability was found to vary in a dose dependent manner with lower viability observed with increasing electric field strength and pulse duration. Cell viability was greater than 83% for all cases except for the most aggressive pulse condition (1[Formula: see text]kV/cm for 5[Formula: see text]ms), where the viability dropped to 67.1%. These studies can assist in determining critical permeabilization and molecular delivery parameters while preserving viability.

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