scholarly journals Expression and Characterization of a Dye-Decolorizing Peroxidase from Pseudomonas Fluorescens Pf0-1

Catalysts ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 463 ◽  
Author(s):  
Nikola Lončar ◽  
Natalija Drašković ◽  
Nataša Božić ◽  
Elvira Romero ◽  
Stefan Simić ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pseudomonas Fluorescens ◽  
Alginate Beads ◽  
Sequence Information ◽  
Soluble Enzyme ◽  
Initial Activity ◽  
Alternative Substrate ◽  
65 H ◽  
Genome Sequence Information

The consumption of dyes is increasing worldwide in line with the increase of population and demand for clothes and other colored products. However, the efficiency of dyeing processes is still poor and results in large amounts of colored effluents. It is desired to develop a portfolio of enzymes which can be used for the treatment of colored wastewaters. Herein, we used genome sequence information to discover a dye-decolorizing peroxidase (DyP) from Pseudomonas fluorescens Pf-01. Two genes putatively encoding for DyPs were identified in the respective genome and cloned for expression in Escherichia coli, of which one (PfDyP B2) could be overexpressed as a soluble protein. PfDyP B2 shows some typical features known for DyPs which includes the ability to convert dyes at the expense of hydrogen peroxide. Interestingly, t-butyl hydroperoxide could be used as an alternative substrate to hydrogen peroxide. Immobilization of PfDyP B2 in calcium-alginate beads resulted in a significant increase in stability: PfDyP B2 retains 80% of its initial activity after 2 h incubation at 50 °C, while the soluble enzyme is inactivated within minutes. PfDyP B2 was also tested with aniline and ethyl diazoacetate as substrates. Based on GC-MS analyses, 30% conversion of the starting material was achieved after 65 h at 30 °C. Importantly, this is the first report of a DyP-catalyzed insertion of a carbene into an N-H bond.

scholarly journals Genetic characterization of the Wyeomyia group of orthobunyaviruses and their phylogenetic relationships

2012 ◽  
Vol 93 (5) ◽  
pp. 1023-1034 ◽  
Author(s):  
Rashmi Chowdhary ◽  
Craig Street ◽  
Amelia Travassos da Rosa ◽  
Marcio R. T. Nunes ◽  
Kok Keng Tee ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
Whole Genome Sequence ◽  
Interferon Response ◽  
Sequence Information ◽  
History Of ◽  
Group Members ◽  
Genome Sequence Information ◽  
Genome Reassortment

Phylogenetic analyses can give new insights into the evolutionary history of viruses, especially of viruses with segmented genomes. However, sequence information for many viral families or genera is still limited and phylogenies based on single or short genome fragments can be misleading. We report the first genetic analysis of all three genome segments of Wyeomyia group viruses Wyeomyia, Taiassui, Macaua, Sororoca, Anhembi and Cachoeira Porteira (BeAr328208) in the genus Orthobunyavirus of the family Bunyaviridae. In addition, Tucunduba and Iaco viruses were identified as members of the Wyeomyia group. Features of Wyeomyia group members that distinguish them from other viruses in the Bunyamwera serogroup and from other orthobunyaviruses, including truncated NSs sequences that may not counteract the host’s interferon response, were characterized. Our findings also suggest genome reassortment within the Wyeomyia group, identifying Macaua and Tucunduba viruses as M-segment reassortants that, in the case of Tucunduba virus, may have altered pathogenicity, stressing the need for whole-genome sequence information to facilitate characterization of orthobunyaviruses and their phylogenetic relationships.

Whole genome genetic-typing in yeast using high-density oligonucleotide arrays

Parasitology ◽  
1999 ◽  
Vol 118 (7) ◽  
pp. 73-80 ◽  
Author(s):  
E. A. WINZELER ◽  
B. LEE ◽  
J. H. McCUSKER ◽  
R. W. DAVIS
Keyword(s):  
New Technologies ◽  
High Density ◽  
Open Reading Frames ◽  
Sequence Information ◽  
Oligonucleotide Arrays ◽  
Genome Sequence Information ◽  
Genome Content ◽  
Reading Frames ◽  
Lower Copy

Genome sequence information in combination with new technologies has allowed researchers to approach genetic problems in new ways. High-density oligonucleotide arrays were used to probe the genome content of the yeast Saccharomyces cerevisiae. We show that these arrays, containing oligonucleotides complementary to the sequenced strain of S. cerevisiae, can be used to identify open reading frames that are missing or present in higher or lower copy number in related isolates of S. cerevisiae. We apply this method to the characterization of the genome of a strain derived from a clinical isolate of S. cerevisiae. Our results show that the telomeres are the regions with the most variability between the two strains.

CHARACTERIZATION OF A HYDROGEN PEROXIDE-BENZENE COMPLEX USING MATRIX ISOLATION INFRARED SPECTROSCOPY

2019 ◽  
Author(s):  
Jay Amicangelo ◽  
Lia Totleben ◽  
Jacob Oslosky ◽  
Yudhishtara Payagala ◽  
Catherine Kaiser ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Infrared Spectroscopy ◽  
Matrix Isolation

Synthesis and Characterization of Silver Nanoplates by a Seed-mediated Method

2019 ◽  
Vol 29 (3) ◽  
Author(s):  
Mai Ngọc Tuan Anh
Keyword(s):  
Hydrogen Peroxide ◽  
Silver Nanoparticles ◽  
Ascorbic Acid ◽  
Silver Nitrate ◽  
Sodium Borohydride ◽  
Trisodium Citrate ◽  
Synthesis And Characterization ◽  
Silver Nanoplates ◽  
Seed Mediated

Silver nanoplates (SNPs) having different size were synthesized by a seed-mediated method. The seeds -silver nanoparticles with 4 – 6 nm diameters were synthesized first by reducing silver nitrate with sodium borohydride in the present of Trisodium Citrate and Hydrogen peroxide. Then these seeds were developed by continue reducing Ag\(^+\) ions with various amount of L-Ascorbic acid to form SNPs. Our analysis showed that the concentratrion of L-Ascorbic acid, a secondary reducing agent, played an important role to form SNPs. In addition, the size and in-plane dipole plasmon resonance wavelenght of silver nanoplates were increased when the concentration of added silver nitrate increased. The characterization of SNPs were studied by UV-Vis, FE-SEM, EDS and TEM methods.

Acanthamoeba Keratitis: Current Status and Urgent Research Priorities

2019 ◽  
Vol 26 (30) ◽  
pp. 5711-5726 ◽  
Author(s):  
Naveed Ahmed Khan ◽  
Ayaz Anwar ◽  
Ruqaiyyah Siddiqui
Keyword(s):  
Rational Design ◽  
Research Priorities ◽  
Acanthamoeba Keratitis ◽  
Current Treatment ◽  
Research Literature ◽  
Current Status ◽  
Bibliographic Databases ◽  
Sequence Information ◽  
Therapeutic Measures ◽  
Genome Sequence Information

Background:First discovered in the early 1970s, Acanthamoeba keratitis has remained a major eye infection and presents a significant threat to the public health, especially in developing countries. The aim is to present a timely review of our current understanding of the advances made in this field in a comprehensible manner and includes novel concepts and provides clear directions for immediate research priorities.Methods:We undertook a search of bibliographic databases for peer-reviewed research literature and also summarized our published results in this field.Results:The present review focuses on novel diagnostic and therapeutic strategies in details which can provide access to management and treatment of Acanthamoeba keratitis. This coupled with the recently available genome sequence information together with high throughput genomics technology and innovative approaches should stimulate interest in the rational design of preventative and therapeutic measures. Current treatment of Acanthamoeba keratitis is problematic and often leads to infection recurrence. Better understanding of diagnosis, pathogenesis, pathophysiology and therapeutic regimens, would lead to novel strategies in treatment and prophylaxis.

A Simple, Green and Fast Ultraviolet Spectrophotometric Method for the Carbamide Peroxide Determination in Dental Whitening Products

2019 ◽  
Vol 15 (2) ◽  
pp. 138-144
Author(s):  
Fabiana Vieira Lima ◽  
Aline Farias ◽  
Cassiana Mendes ◽  
Simone Gonçalves Cardoso ◽  
Marcos Antônio Segatto Silva
Keyword(s):  
Hydrogen Peroxide ◽  
Pharmaceutical Products ◽  
Reference Solution ◽  
Iodometric Titration ◽  
Carbamide Peroxide ◽  
Ultraviolet Detection ◽  
Water As Solvent ◽  
Development And Validation ◽  
Dental Whitening

Background: The carbamide peroxide is the most commonly active ingredient used for home dental whitening products, its quantification in pharmaceutical products is of extreme importance due to the relation with the products potency and the previously related low carbamide peroxide stability. Once, there is only one official carbamide peroxide determination based on iodometric titration, this method is time-consuming and generates a lot of residues. The aim of this study was to carry out development and validation of a simple and fast ultraviolet spectrophotometer assay to quantify an innovative dental whitening gel. Methods: The proposed method was validated according international conference on harmonization guideline. Procedure is based on the iodide/iodine redox chemistry; iodine released through the action of hydrogen peroxide of carbamide peroxide with ultraviolet detection at 350 nm. Results: The procedure was linear in the concentration range of 1.0-4.0 µg/mL, specific to the excipients, robust for the evaluated parameters (variation of wavelength (± 5 nm); reagent addition (± 10%)), showing the results of RSD 1.88 and 0.39% respectively. Repeatability precision was RSD = 1.42%, with accurate RSD = 2.15% by adding reference solution. The assay used only water as solvent for sample preparation. In comparison to the pharmacopeial method, the latter is more time-consuming, as it generates a lot of residues, and it could not quantify small CP dosages. Conclusion: Thus, the proposed method was proved to be suitable to determine carbamide peroxide during the development and characterization of nanoparticle formulations in the present study.

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