scholarly journals 2D- and 3D-Based Intestinal Stem Cell Cultures for Personalized Medicine

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 225 ◽  
Author(s):  
Yuan Liu ◽  
Ye-Guang Chen
Keyword(s):  
Stem Cell ◽  
Personalized Medicine ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Intestinal Stem Cell ◽  
Efficient Treatment ◽  
Different Types ◽  
Potential Benefits ◽  
Males And Females ◽  
2D And 3D

Colorectal cancer (CRC) is one of the most common cancers that have high occurrence and death in both males and females. As various factors have been found to contribute to CRC development, personalized therapies are critical for efficient treatment. To achieve this purpose, the establishment of patient-derived tumor models is critical for diagnosis and drug test. The establishment of three-dimensional (3D) organoid cultures and two-dimensional (2D) monolayer cultures of patient-derived epithelial tissues is a breakthrough for expanding living materials for later use. This review provides an overview of the different types of 2D- and 3D-based intestinal stem cell cultures, their potential benefits, and the drawbacks in personalized medicine in treatment of the intestinal disorders.

scholarly journals Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

Chemosensors ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 6 ◽  
Author(s):  
Georgia Paivana ◽  
Theofylaktos Apostolou ◽  
Sophie Mavrikou ◽  
Dimitris Barmpakos ◽  
Grigoris Kaltsas ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Immobilized Cells ◽  
Neuroblastoma Cell ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Impedance Analysis ◽  
Neural Cells ◽  
Opposite Behavior ◽  
2D And 3D

In this work, the assessment of the interactions of a bioactive substance applied to immobilized cells in either a two-dimensional (2D) or three-dimensional (3D) arrangement mimicking in vivo tissue conditions is presented. In particular, dopamine (DA) was selected as a stimulant for the implementation of an impedance analysis with a specific type of neural cells (murine neuroblastoma). The aim of this study was the extraction of calibration curves at various frequencies with different known dopamine concentrations for the description of the behavior of dopamine applied to 2D and 3D cell cultures. The results present the evaluation of the mean impedance value for each immobilization technique in each frequency. The differential responses showed the importance of the impedance when frequency is applied in both 2D and 3D immobilization cases. More specifically, in 2D immobilization matrix impedance shows higher values in comparison with the 3D cell culture. Additionally, in the 3D case, the impedance decreases with increasing concentration, while in the 2D case, an opposite behavior was observed.

scholarly journals Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures

2016 ◽  
Vol 213 (3) ◽  
pp. 355-369 ◽  
Author(s):  
Paulina S. Mrozowska ◽  
Mitsunori Fukuda
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Small Gtpases ◽  
Cell Polarization ◽  
Rab Gtpases ◽  
3D Cell Cultures ◽  
Polarized Epithelia ◽  
3D Environments ◽  
2D And 3D

MDCK II cells, a widely used model of polarized epithelia, develop into different structures depending on culture conditions: two-dimensional (2D) monolayers when grown on synthetic supports or three-dimensional (3D) cysts when surrounded by an extracellular matrix. The establishment of epithelial polarity is accompanied by transcytosis of the apical marker podocalyxin from the outer plasma membrane to the newly formed apical domain, but its exact route and regulation remain poorly understood. Here, through comprehensive colocalization and knockdown screenings, we identified the Rab GTPases mediating podocalyxin transcytosis and showed that different sets of Rabs coordinate its transport during cell polarization in 2D and 3D structures. Moreover, we demonstrated that different Rab35 effectors regulate podocalyxin trafficking in 2D and 3D environments; trafficking is mediated by OCRL in 2D monolayers and ACAP2 in 3D cysts. Our results give substantial insight into regulation of the transcytosis of this apical marker and highlight differences between trafficking mechanisms in 2D and 3D cell cultures.

scholarly journals The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Styliani Melissaridou ◽  
Emilia Wiechec ◽  
Mustafa Magan ◽  
Mayur Vilas Jain ◽  
Man Ki Chung ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Stem Cell ◽  
Head And Neck ◽  
Treatment Response ◽  
Cell Cultures ◽  
Neck Cancer ◽  
3D Cell Cultures ◽  
2D And 3D

scholarly journals 2D and 3D cell cultures – a comparison of different types of cancer cell cultures

2016 ◽  
Author(s):  
Marta Kapałczyńska ◽  
Tomasz Kolenda ◽  
Weronika Przybyła ◽  
Maria Zajączkowska ◽  
Anna Teresiak ◽  
...  
Keyword(s):  
Cancer Cell ◽  
Cell Cultures ◽  
3D Cell Cultures ◽  
Different Types ◽  
2D And 3D

scholarly journals Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures

2019 ◽  
Vol 7 ◽  
Author(s):  
Markus Pasztorek ◽  
Eva Rossmanith ◽  
Christoph Mayr ◽  
Fabian Hauser ◽  
Jaroslaw Jacak ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Cultures ◽  
Platelet Lysate ◽  
2D And 3D

Two and Three Dimensional Image Registration Based on B-Spline Composition and Level Sets

2017 ◽  
Vol 21 (2) ◽  
pp. 600-622 ◽  
Author(s):  
Chiu Ling Chan ◽  
Cosmin Anitescu ◽  
Yongjie Zhang ◽  
Timon Rabczuk
Keyword(s):  
Signal Processing ◽  
Image Registration ◽  
Level Sets ◽  
Large Deformations ◽  
Three Dimensional ◽  
Computationally Efficient ◽  
3D Images ◽  
B Spline ◽  
Different Types ◽  
2D And 3D

AbstractAmethod for non-rigid image registration that is suitable for large deformations is presented. Conventional registration methods embed the image in a B-spline object, and the image is evolved by deforming the B-spline object. In this work, we represent the image using B-spline and deform the image using a composition approach. We also derive a computationally efficient algorithm for calculating the B-spline coefficients and gradients of the image by adopting ideas from signal processing using image filters. We demonstrate the application of our method on several different types of 2D and 3D images and compare it with existing methods.

scholarly journals Fibronectin-conjugated thermoresponsive nanobridges generate three dimensional human pluripotent stem cell cultures for differentiation towards the neural lineages

2019 ◽  
Vol 38 ◽  
pp. 101441 ◽  
Author(s):  
Linda Harkness ◽  
Xiaoli Chen ◽  
Zhongfan Jia ◽  
Anthony M. Davies ◽  
Michael Monteiro ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Cultures ◽  
Pluripotent Stem Cell ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cell
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