scholarly journals Biomimetic In Vitro Model of Cell Infiltration into Skin Scaffolds for Pre-Screening and Testing of Biomaterial-Based Therapies

Cells ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 917
Author(s):  
Rafael Ballesteros-Cillero ◽  
Evan Davison-Kotler ◽  
Nupur Kohli ◽  
William S. Marshall ◽  
Elena García-Gareta
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Chronic Wounds ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Surrounding Tissue ◽  
Cell Infiltration ◽  
Skin Substitutes ◽  
Vitro Model

Due to great clinical need, research where different biomaterials are tested as 3D scaffolds for skin tissue engineering has increased. In vitro studies use a cell suspension that is simply pipetted onto the material and cultured until the cells migrate and proliferate within the 3D scaffold, which does not mimic the in vivo reality. Our aim was to engineer a novel biomimetic in vitro model that mimics the natural cell infiltration process occurring in wound healing, thus offering a realistic approach when pre-screening and testing new skin substitutes. Our model consists of porous membrane cell culture inserts coated with gelatin and seeded with human dermal fibroblasts, inside which two different commercially available dermal substitutes were placed. Several features relevant to the wound healing process (matrix contraction, cell infiltration and proliferation, integration of the biomaterial with the surrounding tissue, and secretion of exogenous cytokines and growth factors) were evaluated. Our results showed that cells spontaneously infiltrate the materials and that our engineered model is able to induce and detect subtle differences between different biomaterials. The model allows for room for improvements or “adds-on” and miniaturization and can contribute to the development of functional and efficient skin substitutes for burns and chronic wounds.

scholarly journals 3D In vitro model of the re-epithelialization phase in the wound-healing process

2017 ◽  
Vol 27 (5) ◽  
pp. 460-462 ◽  
Author(s):  
Nathalie Deshayes ◽  
Fabienne Bloas ◽  
Florian Boissout ◽  
Jennifer Lecardonnel ◽  
Maryline Paris
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals Nanotechnology-Based Medical Devices for the Treatment of Chronic Skin Lesions: From Research to the Clinic

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 815
Author(s):  
Marco Ruggeri ◽  
Eleonora Bianchi ◽  
Silvia Rossi ◽  
Barbara Vigani ◽  
Maria Cristina Bonferoni ◽  
...  
Keyword(s):  
Wound Healing ◽  
Medical Devices ◽  
Chronic Wounds ◽  
Healing Process ◽  
Skin Lesions ◽  
In Vitro Tests ◽  
Skin Substitutes ◽  
Nanofibrous Scaffolds ◽  
Recent Developments

Chronic wounds, such as pressure ulcers, diabetic ulcers, venous ulcers and arterial insufficiency ulcers, are lesions that fail to proceed through the normal healing process within a period of 12 weeks. The treatment of skin chronic wounds still represents a great challenge. Wound medical devices (MDs) range from conventional and advanced dressings, up to skin grafts, but none of these are generally recognized as a gold standard. Based on recent developments, this paper reviews nanotechnology-based medical devices intended as skin substitutes. In particular, nanofibrous scaffolds are promising platforms for wound healing, especially due to their similarity to the extracellular matrix (ECM) and their capability to promote cell adhesion and proliferation, and to restore skin integrity, when grafted into the wound site. Nanotechnology-based scaffolds are emphasized here. The discussion will be focused on the definition of critical quality attributes (chemical and physical characterization, stability, particle size, surface properties, release of nanoparticles from MDs, sterility and apyrogenicity), the preclinical evaluation (biocompatibility testing, alternative in vitro tests for irritation and sensitization, wound healing test and animal wound models), the clinical evaluation and the CE (European Conformity) marking of nanotechnology-based MDs.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

099 "Smart" Targeting of Anti?Scarring Agents in an in vitro Model of Retinal Wound Healing

2004 ◽  
Vol 12 (2) ◽  
pp. A26-A26
Author(s):  
F E Dhawahir ◽  
C Sheridan ◽  
D Kent ◽  
D Wong ◽  
I Grierson ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Overcoming the Inflammatory Stage of Non-Healing Wounds: In Vitro Mechanism of Action of Negatively Charged Microspheres (NCMs)

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1108
Author(s):  
Edorta Santos-Vizcaino ◽  
Aiala Salvador ◽  
Claudia Vairo ◽  
Manoli Igartua ◽  
Rosa Maria Hernandez ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanism Of Action ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Control Group ◽  
Promote Cell Proliferation ◽  
Inflammatory Phase ◽  
Inflammatory State ◽  
Diabetic Wounds

Negatively charged microspheres (NCMs) represent a new therapeutic approach for wound healing since recent clinical trials have shown NCM efficacy in the recovery of hard-to-heal wounds that tend to stay in the inflammatory phase, unlocking the healing process. The aim of this study was to elucidate the NCM mechanism of action. NCMs were extracted from a commercial microsphere formulation (PolyHeal® Micro) and cytotoxicity, attachment, proliferation and viability assays were performed in keratinocytes and dermal fibroblasts, while macrophages were used for the phagocytosis and polarization assays. We demonstrated that cells tend to attach to the microsphere surface, and that NCMs are biocompatible and promote cell proliferation at specific concentrations (50 and 10 NCM/cell) by a minimum of 3 fold compared to the control group. Furthermore, NCM internalization by macrophages seemed to drive these cells to a noninflammatory condition, as demonstrated by the over-expression of CD206 and the under-expression of CD64, M2 and M1 markers, respectively. NCMs are an effective approach for reverting the chronic inflammatory state of stagnant wounds (such as diabetic wounds) and thus for improving wound healing.

scholarly journals Adipose Tissue-Derived Mesenchymal Cells Support Skin Reepithelialization through Secretion of KGF-1 and PDGF-BB: Comparison with Dermal Fibroblasts

2012 ◽  
Vol 21 (11) ◽  
pp. 2441-2454 ◽  
Author(s):  
Vassilia-Ismini Alexaki ◽  
Despoina Simantiraki ◽  
Marianna Panayiotopoulou ◽  
Olga Rasouli ◽  
Maria Venihaki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Normal Skin ◽  
Human Adipose Tissue ◽  
Mesenchymal Cells ◽  
Dermal Fibroblasts ◽  
Skin Substitutes ◽  
Keratinocyte Proliferation

Epidermal organization and homeostasis are regulated by mesenchymal influences through paracrine actions. Until today, dermal fibroblasts (DFs) are used in the “dermal” layer to support keratinocyte growth in vitro in dermal and skin substitutes. In the present work, we used human adipose tissue-derived mesenchymal cells (ADMCs) as a support of keratinocyte growth in vitro (in monolayer culture and in 3D skin cell culture models) and in vivo (mouse wound healing models) and compared our findings with those obtained using dermal fibroblasts. ADMCs induce reepithelialization during wound healing more efficiently than DFs, by enhancing keratinocyte proliferation through cell cycle progression, and migration. This effect is mediated (at least partially) by a paracrine action of KGF-1 and PDGF-BB, which are more prominently expressed in ADMCs than in DFs. Furthermore, replacement of DFs by ADMCs in the dermal compartment of organotypic skin cultures leads to an artificial epidermis resembling to that of normal skin, concerning the general histology, although with a higher expression of cytokeratins 5 and 19. In Rag1 knockout mice, ADMCs induced a more rapid reepithelialization and a more effective wound healing, compared to dermal fibroblasts. In conclusion, we provide evidence that ADMCs can serve as supportive cells for primary keratinocyte cultures. In addition, because of their abundance and the great cell yield achieved during ADMC isolation, they represent an interesting cell source, with potential aspects for clinical use.

scholarly journals Insulin catalyzes the curcumin-induced wound healing: An in vitro model for gingival repair

2012 ◽  
Vol 44 (4) ◽  
pp. 458 ◽  
Author(s):  
AnilK Balapure ◽  
Jaya Dixit ◽  
Divya Lodha ◽  
Vishal Ranjan ◽  
Ramesh Sharma ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Bioelectric modulation of wound healing in a 3D in vitro model of tissue-engineered bone

Biomaterials ◽  
2013 ◽  
Vol 34 (28) ◽  
pp. 6695-6705 ◽  
Author(s):  
Sarah Sundelacruz ◽  
Chunmei Li ◽  
Young Jun Choi ◽  
Michael Levin ◽  
David L. Kaplan
Keyword(s):  
Wound Healing ◽  
In Vitro Model ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals A new in vitro model of venous hypertension: the effect of pressure on dermal fibroblasts

2003 ◽  
Vol 38 (5) ◽  
pp. 1099-1105 ◽  
Author(s):  
Chris Healey ◽  
Patrick Forgione ◽  
Karen M. Lounsbury ◽  
Kim Corrow ◽  
Turner Osler ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Venous Hypertension ◽  
Dermal Fibroblasts ◽  
Effect Of Pressure ◽  
Vitro Model
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