Nanoparticle-Lipid Interaction: Job Scattering Plots to Differentiate Vesicle Aggregation from Supported Lipid Bilayer Formation

The impact of nanomaterials on lung fluids or on the plasma membrane of living cells has prompted researchers to examine the interactions between nanoparticles and lipid vesicles. Recent studies have shown that nanoparticle-lipid interaction leads to a broad range of structures including supported lipid bilayers (SLB), particles adsorbed at the surface or internalized inside vesicles, and mixed aggregates. Today, there is a need to have simple protocols that can readily assess the nature of structures obtained from particles and vesicles. Here we apply the method of continuous variation for measuring Job scattering plots and provide analytical expressions for the scattering intensity in various scenarios. The result that emerges from the comparison between modeling and experimental measurements is that electrostatics plays a key role in the association, but it is not sufficient to induce the formation of supported lipid bilayers.

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Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

10.26434/chemrxiv.7505330 ◽

2018 ◽

Author(s):

Luke Jordan ◽

Nathan Wittenberg

Keyword(s):

Lipid Bilayers ◽

Binding Kinetics ◽

Supported Lipid Bilayers ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Supported Lipid Bilayer ◽

Head Groups ◽

Lipid Diffusion ◽

Kinetics Of ◽

Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

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The role of surface charge in the interaction of nanoparticles with model pulmonary surfactants

Soft Matter ◽

10.1039/c8sm00925b ◽

2018 ◽

Vol 14 (28) ◽

pp. 5764-5774 ◽

Cited By ~ 11

Author(s):

F. Mousseau ◽

J.-F. Berret

Keyword(s):

Surface Charge ◽

Lipid Bilayers ◽

Pulmonary Surfactant ◽

Lipid Vesicles ◽

Electrostatic Charge ◽

Supported Lipid Bilayers ◽

Pulmonary Surfactants ◽

Inhaled Nanoparticles

Inhaled nanoparticles reaching the respiratory zone in the lungs enter first in contact with the pulmonary surfactant. It is shown here that nanoparticles and lipid vesicles formulated from different surfactant mimetics interact predominantlyviaelectrostatic charge mediated attraction and do not form supported lipid bilayers spontaneously.

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Correlative microscopy reveals the nanoscale morphology of E. coli-derived supported lipid bilayers

10.1101/2021.11.04.467316 ◽

2021 ◽

Author(s):

Karan Bali ◽

Zeinab Mohamed ◽

Anna-Maria Pappa ◽

Susan Daniel ◽

Clemens F. Kaminski ◽

...

Keyword(s):

Lipid Bilayers ◽

Lipid Membrane ◽

Lipid Vesicles ◽

Outer Membrane Vesicles ◽

Atomic Scale ◽

Membrane Properties ◽

Supported Lipid Bilayers ◽

Structured Illumination ◽

Structured Illumination Microscopy ◽

Natural And Synthetic

Supported lipid bilayers (SLBs) made from reconstituted lipid vesicles are an important tool in molecular biology. A breakthrough in the field has come with the use of vesicles derived from cell membranes to form SLBs. These new supported bilayers, consisting both of natural and synthetic components, provide a physiologically relevant system on which to study protein-protein interactions as well as protein-ligand interactions and other lipid membrane properties. These complex bilayer systems hold promise but have not yet been fully characterised in terms of their composition, ratio of natural to synthetic component and membrane protein content. Here, we describe a method of correlative atomic force (AFM) with structured illumination microscopy (SIM) for the accurate mapping of complex lipid bilayers that consist of a synthetic fraction and a fraction of lipids derived from Escherichia coli outer membrane vesicles (OMVs). We exploit the enhanced resolution and molecular specificity that SIM can offer to identify areas of interest in these bilayers and the atomic scale resolution that the AFM provides to create detailed topography maps of the bilayers. We are thus able to understand the way in which the two different lipid fractions (natural and synthetic) mix within the bilayers, quantify the amount of bacterial membrane incorporated in the bilayer and directly visualise the interaction of these bilayers with bacteria-specific, membrane-binding proteins. Our work sets the foundation for accurately understanding the composition and properties of OMV-derived SLBs and establishes correlative AFM/ SIM as a method for characterising complex systems at the nanoscale.

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Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

Biophysical Journal ◽

10.1016/j.bpj.2009.12.3698 ◽

2010 ◽

Vol 98 (3) ◽

pp. 673a

Author(s):

Lisa V. Simonsson ◽

Peter Jönsson ◽

Gudrun Stengel ◽

Fredrik Höök

Keyword(s):

Lipid Bilayers ◽

Lipid Vesicles ◽

Supported Lipid Bilayers ◽

Site Specific ◽

Controlled Fusion

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Supported lipid bilayer repair mediated by AH peptide

Physical Chemistry Chemical Physics ◽

10.1039/c5cp06472d ◽

2016 ◽

Vol 18 (4) ◽

pp. 3040-3047 ◽

Cited By ~ 8

Author(s):

Min Chul Kim ◽

Anders Gunnarsson ◽

Seyed R. Tabaei ◽

Fredrik Höök ◽

Nam-Joon Cho

Keyword(s):

Lipid Bilayer ◽

Lipid Bilayers ◽

Silicon Oxide ◽

Supported Lipid Bilayers ◽

High Quality ◽

Supported Lipid Bilayer

High quality and complete supported lipid bilayers are formed on silicon oxide by employing an AH peptide mediated repair step.

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Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

10.26434/chemrxiv.7505330.v1 ◽

2018 ◽

Author(s):

Luke Jordan ◽

Nathan Wittenberg

Keyword(s):

Lipid Bilayers ◽

Binding Kinetics ◽

Supported Lipid Bilayers ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Supported Lipid Bilayer ◽

Head Groups ◽

Lipid Diffusion ◽

Kinetics Of ◽

Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

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Site-Specific DNA-Controlled Fusion of Single Lipid Vesicles to Supported Lipid Bilayers

ChemPhysChem ◽

10.1002/cphc.200901010 ◽

2010 ◽

Vol 11 (5) ◽

pp. 1011-1017 ◽

Cited By ~ 25

Author(s):

Lisa Simonsson ◽

Peter Jönsson ◽

Gudrun Stengel ◽

Fredrik Höök

Keyword(s):

Lipid Bilayers ◽

Lipid Vesicles ◽

Supported Lipid Bilayers ◽

Site Specific ◽

Controlled Fusion

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A dynamic 3D DNA nanostructure based on silicon-supported lipid bilayers: a highly efficient DNA nanomachine for rapid and sensitive sensing

Chemical Communications ◽

10.1039/c9cc07071k ◽

2019 ◽

Vol 55 (89) ◽

pp. 13414-13417 ◽

Cited By ~ 6

Author(s):

Zhi-Bin Wen ◽

Xin Peng ◽

Ze-Zhou Yang ◽

Ying Zhuo ◽

Ya-Qin Chai ◽

...

Keyword(s):

Lipid Bilayers ◽

Three Dimensional ◽

Dna Probes ◽

Supported Lipid Bilayers ◽

Dna Nanostructure ◽

Highly Efficient ◽

Self Powered ◽

Lipid Interaction ◽

Dna Nanomachine

Herein, we have developed a dynamic three-dimensional (3D) self-powered DNA nanomachine by anchoring cholesterol-labelled DNA probes to silicon-supported lipid bilayers via cholesterol–lipid interaction.

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Nanoscale Patterning of Antigen on Silicon Substrate to Examine Mast Cell Activation

MRS Proceedings ◽

10.1557/proc-724-n4.3 ◽

2002 ◽

Vol 724 ◽

Author(s):

Reid N. Orth ◽

Min Wu ◽

Theodore G. Clark ◽

David A. Holowka ◽

Barbara A. Baird ◽

...

Keyword(s):

Lipid Bilayers ◽

Cell Activation ◽

Lipid Vesicles ◽

Cell Surface Receptor ◽

Mast Cell Activation ◽

Supported Lipid Bilayer ◽

Nanoscale Patterning ◽

Surface Receptor ◽

Biological Substrate ◽

Rbl Cells

AbstractRat Basophilic Leukemia (RBL) cells are immobilized and stimulated on micro- and nanometer scale patterns of supported lipid bilayers. The patterns are realized as the photolithographically patterned polymer is mechanically peeled away in one contiguous piece in solution. The 0.36 μm2 to 4, 489 μm2 patches can contain both fluorescent lipids and lipid-linked antigen and provide a synthetic biological substrate for analysis of cell surface receptor-mediated events. 100-nm unilamellar lipid vesicles spread to form a supported lipid bilayer on a thermally oxidized silicon surface as confirmed by fluorescence recovery after photobleaching (FRAP). Aggregation of fluorescently labeled receptors is observed as their coincidence with the patterned antigen. Cell morphology is analyzed with scanning electron microscopy (SEM). Thus, a novel method has been developed for patterning antigen, capturing and immobilizing cells via specific receptors, and spatially controlling antigenic stimulus on the nanoscale.

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