scholarly journals Isolation and Identification of Endophytic Bacteria from Mycorrhizal Tissues of Terrestrial Orchids from Southern Chile

Diversity ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 55 ◽  
Author(s):  
Héctor Herrera ◽  
Tedy Sanhueza ◽  
Alžběta Novotná ◽  
Trevor C. Charles ◽  
Cesar Arriagada
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Developmental Stages ◽  
Pathogenic Fungi ◽  
Southern Chile ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Acetic Acid Production ◽  
Terrestrial Orchids ◽  
Plant Growth Promoting

Endophytic bacteria are relevant symbionts that contribute to plant growth and development. However, the diversity of bacteria associated with the roots of terrestrial orchids colonizing Andean ecosystems is limited. This study identifies and examines the capabilities of endophytic bacteria associated with peloton-containing roots of six terrestrial orchid species from southern Chile. To achieve our goals, we placed superficially disinfected root fragments harboring pelotons on oatmeal agar (OMA) with no antibiotic addition and cultured them until the bacteria appeared. Subsequently, they were purified and identified using molecular tools and examined for plant growth metabolites production and antifungal activity. In total, 168 bacterial strains were isolated and assigned to 8 OTUs. The orders Pseudomonadales, Burkholderiales, and Xanthomonadales of phylum Proteobacteria were the most frequent. The orders Bacillales and Flavobacteriales of the phylla Firmicutes and Bacteroidetes were also obtained. Phosphate solubilization was detected in majority of isolates; however, it was significantly higher in Collimonas pratensis and Chryseobacterium sp. (PSI = 1.505 ± 0.09 and 1.405 ± 0.24, respectively). Siderophore production was recorded only for C. pratensis (0.657 ± 0.14 mm day−1), Dyella marensis (0.131 ± 0.02 mm day−1), and Luteibacter rhizovicinus (0.343 ± 0.12 mm day−1). Indole acetic acid production was highly influenced by the isolate identity; however, the significantly higher activity was recorded for Pseudomonas spp. (ranging from 5.507 ± 1.57 µg mL−1 to 7.437 ± 0.99 µg mL−1). Additionally, six bacterial isolates were able to inhibit the growth of some potential plant pathogenic fungi. Our findings demonstrate the potential for plant growth promoting capabilities and some antifungal activities of endophytic bacteria inhabiting the mycorrhizal tissue of terrestrial orchids, which may contribute especially at early developmental stages of orchid seedlings.

scholarly journals Biocontrol potential; antifungal activity and plant growth promoting activities of endophytic bacteria from Raphanus sativus

2021 ◽  
Vol 12 (2) ◽  
pp. 1143-1150
Author(s):  
Lavanya J ◽  
Chanthosh S ◽  
Reshma Shrii ◽  
Viknesh V ◽  
Deepika S ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Raphanus Sativus ◽  
Endophytic Bacteria ◽  
Effective Strain ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Plant Pathogenic Fungi ◽  
Plant Growth Promoting ◽  
Growth Promoting

The study was aimed to find an alternate approach for chemicals used in agriculture to avoid microbial infections. Fungal pathogens cause different types of plant diseases and affect a majority of edible crops by destroying the tissues of the plant in a direct or indirect mechanism. So, an alternative approach led to the development of biocontrol agents using endophytic  bacteria. A total of 8 endophytic bacteria were isolated from the root, stem, and leaves of radish (Raphanus sativus). The antagonistic activity of these bacteria against the 2 isolated plant pathogenic fungi was determined in vitro. Two out of eight bacteria showed more than 50% inhibitory activity against one fungus, were further characterized using the 16s rRNA sequencing method. On the basis of the phylogenetic tree of the 16s rRNA method, the endophytic bacterial samples were identified as Tonsilliphilus suis  and Exiguobacterium aurantiacum against plant pathogenic Aspergillus flavus  isolated from Raphanus sativus, which makes them highly suitable as an alternative for chemical fertilizers to provide resistance to plant pathogenic fungi. The cell wall degrading activities such as protease activity, amylase activity, and plant growth-promoting properties such as Hydrogen cyanide (HCN), Indole acetic acid (IAA), ammonia production of these endophytic bacteria were evaluated. The results show that T. suis  is the most effective strain for radish growth development.

scholarly journals IDENTIFICATION OF ENDOPHYTIC BACTERIA OF SEEDS FROM Cedrela odorata L. (Meliaceae) WITH BIOTECHNOLOGICAL CHARACTERISTICS

2021 ◽  
Vol 26 (2) ◽  
pp. 196-206
Author(s):  
Saúl Espinosa Zaragoza ◽  
Ricardo Sánchez Cruz ◽  
Diana Sanzón Gómez ◽  
Margarita C Escobar Sandoval ◽  
Gustavo Yañez Ocampo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Carbon Sources ◽  
Phytopathogenic Fungi ◽  
Ribosomal Gene ◽  
Cedrela Odorata ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Xenobiotic Compounds ◽  
Phosphate Solubilizing

In the present study, 62 endophytic bacterial strains of cedar seeds (Cedrela odorataL.), collected in the municipalities of Huehuetán, Motozintla, and Pijijiapan in the state of Chiapas, Mexico were isolated. The goal was to identify characteristics of biotechnological interest such as biocontrol, promotion of plant growth, and growth in aromatic compounds. The strains were identified by the partial sequence of the 16S ribosomal gene as belonging to the Bacillusgenus. The biocontrol capacity of phytopathogenic fungi, production of indoleacetic acid (IAA), solubilization of phosphate, and growth in xenobiotic compounds (phenanthrene, benzene, anthracene, or phenol) were detected in 26 strains of the 62 isolates. 21 % of the strains inhibited the mycelial growth of Alternaria solaniand Fusariumsp., and 13 % of the Phytophthora capsicioomycete. IAA production was detected in 24 isolates, phosphate solubilizing activity was identified in 18 isolates, while the ability to grow in the presence of phenanthrene and benzene was found in 26 isolates; 24 isolates grew in the presence of anthracene and only two isolates grew in phenol as the only carbon sources. This is the first report of the isolation and identification of endophytic bacteria from cedar seeds, where biotechnological characteristics were detected for biological control, promotion of plant growth, and growth in the presence of xenobiotic compounds.

scholarly journals Mediterranean Native Leguminous Plants: A Reservoir of Endophytic Bacteria with Potential to Enhance Chickpea Growth under Stress Conditions

2019 ◽  
Vol 7 (10) ◽  
pp. 392 ◽  
Author(s):  
Clarisse Brígido ◽  
Esther Menéndez ◽  
Ana Paço ◽  
Bernard R. Glick ◽  
Anabela Belo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Total Biomass ◽  
Plant Growth Promoting Bacteria ◽  
Legume Species ◽  
Bacterial Endophytes ◽  
Acetic Acid Production ◽  
Plant Growth Promoting ◽  
Native Legume ◽  
Growth Promoting

Bacterial endophytes, a subset of a plant’s microbiota, can facilitate plant growth by a number of different mechanisms. The aims of this study were to assess the diversity and functionality of endophytic bacterial strains from internal root tissues of native legume species grown in two distinct sites in South of Portugal and to evaluate their ability to promote plant growth. Here, 122 endophytic bacterial isolates were obtained from 12 different native legume species. Most of these bacteria possess at least one of the plant growth-promoting features tested in vitro, with indole acetic acid production being the most common feature among the isolates followed by the production of siderophores and inorganic phosphate solubilization. The results of in planta experiments revealed that co-inoculation of chickpea plants with specific endophytic bacteria along with N2-fixing symbionts significantly improved the total biomass of chickpea plants, in particular when these plants were grown under saline conditions. Altogether, this study revealed that Mediterranean native legume species are a reservoir of plant growth-promoting bacteria, that are also tolerant to salinity and to toxic levels of Mn. Thus, these bacterial endophytes are well adapted to common constraints present in soils of this region which constitutes important factors to consider in the development of bacterial inoculants for stressful conditions in the Mediterranean region.

scholarly journals Isolation and Characterization of Plant Growth Promoting Endophytic Bacteria from Desert Plants and Their Application as Bioinoculants for Sustainable Agriculture

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1325 ◽  
Author(s):  
Muneera D. F. ALKahtani ◽  
Amr Fouda ◽  
Kotb A. Attia ◽  
Fahad Al-Otaibi ◽  
Ahmed M. Eid ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Species ◽  
Desert Plants ◽  
Bacterial Endophytes ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

Desert plants are able to survive under harsh environmental stresses inherent to arid and semiarid regions due to their association with bacterial endophytes. However, the identity, functions, and the factors that influence the association of bacterial endophytes with desert plants are poorly known. These bacterial endophytes can be used as an untapped resource to favor plant growth and development in agro-ecosystems of arid regions. The present study is therefore focused on the isolation and identification of bacterial endophytes from two native medicinal plants (Fagonia mollis Delile and Achillea fragrantissima (Forssk) Sch. Bip.) growing spontaneously in the arid region of the South Sinai (Egypt), and characterization of their plant growth promoting (PGP) traits. Thirteen putative bacterial endophytes were isolated from the leaves of both plant species and characterized for their plant growth promoting abilities using molecular and biochemical approaches, as well as greenhouse trials. Selected endophytic bacterial strains were applied to maize plants (Zea mays L. var. Single cross Pioneer 30K08) to further evaluate their PGP abilities under greenhouse conditions. Isolated bacterial strains have variable plant growth promoting activities. Among these activities, isolated bacterial endophytes have the efficacy of phosphate solubilizing with clear zones ranging from 7.6 ± 0.3 to 9.6 ± 0.3 mm. Additionally, the obtained bacterial endophytes increased the productivity of indole acetic acid (IAA) in broth media from 10 to 60 µg·mL−1 with increasing tryptophan concentration from 1 to 5 mg·mL−1. Bacillus and Brevibacillus strains were frequently isolated from the leaves of both plant species, and had significant positive effects on plant growth and shoot phosphorus (P) and nitrogen (N) contents. Results suggest that these endophytes are good candidates as plant growth promoting inoculants to help reduce chemical input in conventional agricultural practices and increase nutrient uptake and stress resilience in plant species.

scholarly journals Simultaneous Removal of Reactive Dyes and Hexavalent Chromium by a Metal Tolerant Pseudomonas sp. WS-D/183 Harboring Plant Growth Promoting Traits

2020 ◽  
Vol 23 (02) ◽  
pp. 241-252
Author(s):  
Sabir Hussain
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Treatment Strategies ◽  
Reactive Dyes ◽  
Pseudomonas Sp ◽  
Bacterial Strains ◽  
Acetic Acid Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Textile industry is a continuous source of colored wastewater. This wastewater frequently used for irrigation purpose in many underdeveloped countries including Pakistan. In this study, we isolated the bacterial strains capable of decolorizing dyes and promote the plant growth. Hence to decolorize the reactive red 120 (RR120), the strain WS-D/183 was optimized following response surface methodology (RSM) based modeling approach. Moreover, strain WS-D/183 was also assessed for plant growth promoting characteristics. Results revealed that the strain WS-D/183 showed a good potential for decolorization of structurally diverse types of azo dyes on reaction with a mixture of heavy metal ions (Cr6+, Cd2+, Zn2+, Pb2+). This strain concurrently removed reactive dyes (100 mg L-1 ) and reduced Cr(VI). Results showed that each dye was decolorized up to 90% except reactive yellow-2 which was decolorized up to 57.4%. Furthermore, the bacterium reduced Cr(VI) by 41 to 95% along with concurrent decolorization of RR120. This bacterium was also found to carry plant growth promoting traits including inorganic phosphate solubilization (497.6 ± 14.8 µg mL-1 ) and indole-3-acetic acid production (21.07 ± 0.9 µg mL-1 ). A phytotoxicity evaluation study indicated that irrigation of mung bean [Vigna radiata (L.) Wilczek] with RR120, Cr(VI) and RR120+Cr(VI) contaminated waters treated with the strain WS-D/183 enhanced germination along with plumule and radical length of seedlings. Results suggested that Pseudomonas sp. WS-D/183 is a valuable addition to the bioresources, which can be used to devise textile wastewater treatment strategies as well as for integrated bioremediation and plant growth promotion in agricultural soils contaminated with textile wastewaters. © 2020 Friends Science Publishers

scholarly journals Isolation and identification of chromium-tolerant bacterial strains and their potential to promote plant growth

2019 ◽  
Vol 96 ◽  
pp. 01005
Author(s):  
Muhammad Arshad ◽  
Anza Javaid ◽  
Maria Manzoor ◽  
Kiran Hina ◽  
Muhammad Arif Ali ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Pseudomonas Plecoglossicida ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Remediation and management of Chromium (Cr) contaminated soils for safe usage has been a serious challenge. The aim of present work was to isolate and identify Cr tolerant bacterial strains and assessing their plant growth promoting potential under controlled conditions. Soil samples were collected from Gujrat District, Pakistan, and analyzed for Cr content and bacteria isolation. In-vitro screening was done for chromium tolerance and plant growth-promoting (PGP) abilities. The tolerant isolates which also exhibited PGP abilities were used as inoculants in germination and pot trial experiment. The isolates A5 and A6, identified as Pseudomonas plecoglossicida and Staphylococci saprophyticus through 16S rRNA gene sequencing, were found to be tolerant up to 700 mg L-1 of Cr (VI). Both were effective in solubilizing phosphate but only A5 (Pseudomonas plecoglossicida) was able to produce indole acetic acid. A5 also increased the percentage of seed germination from 17 to 46% and spinach plant’s biomass by 44% with respect to the control. Keeping in view the results obtained, A5 (Pseudomonas plecoglossicida) appeared as the best species that was able to tolerate Cr stress and promote plant growth. Further, it can be developed as a bio-inoculant for non-food agricultural applications for remediation of Cr contaminated soils.

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