scholarly journals Chronic Hindlimb Ischemia Assessment; Quantitative Evaluation Using Laser Doppler in a Rodent Model of Surgically Induced Peripheral Arterial Occlusion

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 139 ◽  
Author(s):  
Hoinoiu ◽  
Jiga ◽  
Nistor ◽  
Dornean ◽  
Barac ◽  
...  
Keyword(s):  
Quantitative Evaluation ◽  
Popliteal Artery ◽  
Arterial Occlusion ◽  
Tissue Perfusion ◽  
Laser Doppler ◽  
Average Weight ◽  
Hindlimb Ischemia ◽  
Chronic Ischemia ◽  
Peripheral Arterial ◽  
Doppler Analysis

Therapeutic neoangiogenesis (TNA) holds promise as a treatment for peripheral arterial disease. Nevertheless, proper tools for in vivo pre-clinical investigation of different TNA approaches and their effects are still lacking. Here we describe a chronic ischemic hindlimb model in rats using laser Doppler quantitative evaluation of tissue perfusion. Male Wistar rats (n = 20), aged between 6–8 months, with an average weight of 287 ± 26.74 g, were used. Animals were divided into two experimental groups: group A (n = 17; hindlimb chronic ischemia model) and group B (n = 3; control). Hindlimb ischemia was induced by concomitant ligation of the right femoral and popliteal artery. Evaluation of tissue perfusion was quantified in perfusion units (PU) on a scale from 0 to 500 (500 PU = maximal detectable perfusion) by laser Doppler analysis at day 0, day 15 and day 30 after induction of ischemia. Induction of chronic ischemia in the rat hindlimb by concomitant ligation of the femoral and popliteal artery can be readily obtained but requires basic microsurgical skills. Laser Doppler analysis has shown unaltered ischemia levels throughout the study (129,17 PU ± 3.13 day 0 vs. 130,33 PU day 30 ± 3,27, p = not significant (n.s.)). We demonstrate a simple and reproducible model of chronic hindlimb ischemia in rats, with stable tissue perfusion levels that are accurately quantified using laser Doppler technology. Hence, this model can represent a valid tool for further studies involving therapeutic neoangiogenesis.

A New Intra Arterial rt-PA Dosage Regimen in Peripheral Arterial Occlusion: Bolus Followed by Continuous Infusion

1991 ◽  
Vol 65 (05) ◽  
pp. 635-635 ◽  
Author(s):  
Claude Juhan ◽  
Serge Haupert ◽  
Gilles Miltgen ◽  
Nadine Girard ◽  
Pierre Dulac
Keyword(s):  
Continuous Infusion ◽  
Dosage Regimen ◽  
Arterial Occlusion ◽  
Peripheral Arterial ◽  
Peripheral Arterial Occlusion

scholarly journals Notch signaling-modified mesenchymal stem cells improve tissue perfusion by induction of arteriogenesis in a rat hindlimb ischemia model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shusaku Maeda ◽  
Shigeru Miyagawa ◽  
Takuji Kawamura ◽  
Takashi Shibuya ◽  
Kenichi Watanabe ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Tissue Perfusion ◽  
Human Mesenchymal Stem Cell ◽  
Capillary Density ◽  
Adductor Muscle ◽  
Notch Signaling Pathway ◽  
Hindlimb Ischemia ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Whole Transcriptome Analysis

AbstractNotch signaling-modified human mesenchymal stem cell, SB623 cell, is a promising cell therapy product for ischemic stroke. With the aim to expand indications for their use for critical limb-threatening ischemia (CLTI), we hypothesized that SB623 cells improved tissue perfusion by inducing angiogenesis or arteriogenesis in a hindlimb ischemia model rat. In Sprague–Dawley rats, hindlimb ischemia was generated by femoral artery removal, then seven days after ischemic induction 1 × 105 SB623 cells or PBS was injected into the ischemic adductor muscle. As compared with the PBS group, tissue perfusion was significantly increased in the SB623 group. While capillary density did not vary between the groups, αSMA- and vWF-positive arterioles with a diameter  > 15 μm were significantly increased in the SB623 group. Whole transcriptome analysis of endothelial cells co-cultured with SB623 cells showed upregulation of the Notch signaling pathway as well as several other pathways potentially leading to arteriogenesis. Furthermore, rat muscle treated with SB623 cells showed a trend for higher ephrin-B2 and significantly higher EphB4 expression, which are known as arteriogenic markers. In the hindlimb ischemia model, SB623 cells improved tissue perfusion by inducing arteriogenesis, suggesting a promising cell source for treatment of CLTI.

scholarly journals Laser Doppler blood flowmeter as a useful instrument for the early detection of lower extremity peripheral arterial disease in hemodialysis patients: an observational study

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Takeo Ishii ◽  
Shizuka Takabe ◽  
Yuki Yanagawa ◽  
Yuko Ohshima ◽  
Yasuhiro Kagawa ◽  
...  
Keyword(s):  
Blood Flow ◽  
Peripheral Arterial Disease ◽  
Early Stage ◽  
Arterial Disease ◽  
Hemodialysis Patients ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
Skin Perfusion ◽  
Dorsal Area ◽  
Peripheral Arterial

Abstract Background A simpler method for detecting atherosclerosis obliterans is required in the clinical setting. Laser Doppler flowmetry (LDF) is easy to perform and can accurately detect deterioration in skin perfusion. We performed LDF for hemodialysis patients to determine the correlations between blood flow in the lower limbs and peripheral arterial disease (PAD). Methods This retrospective study included 128 hemodialysis patients. Patients were categorized into the non-PAD group (n = 106) and PAD group (n = 22), 14 early stage PAD patients were included in the PAD group. We conducted LDF for the plantar area and dorsal area of the foot and examined skin perfusion pressure (SPP) during dialysis. Results SPP-Dorsal Area values were 82.1 ± 22.0 mmHg in the non-PAD, and 59.1 ± 20.3 mmHg in PAD group, respectively (p < 0.05). The LDF-Plantar blood flow (Qb) values were 32.7 ± 15.5 mL/min in non-PAD group and 21.5 ± 11.3 mL/min in PAD group (p < 0.001). A total of 21 non-PAD patients underwent LDF before and during dialysis. The LDF-Plantar-Qb values were 36.5 ± 17.6 mL/min before dialysis and 29.6 ± 17.7 mL/min after dialysis (p < 0.05). We adjusted SPP and LDF for PAD using logistic regression, SPP-Dorsal-Area and LDF-P were significantly correlated with PAD (p < 0.05). The receiver-operating characteristic curve analysis indicated cut-off values of 20.0 mL/min for LDF-Plantar-Qb during dialysis. Conclusion LDF is a simple technique for sensitive detection of early-stage PAD. This assessment will help physicians identify early-stage PAD, including Fontaine stage II in clinical practice, thereby allowing prompt treatment.

Antiplatelet Therapy Using Cilostazol, a Specific PDE3 Inhibitor

1999 ◽  
Vol 82 (08) ◽  
pp. 435-438 ◽  
Author(s):  
Yasuo Ikeda
Keyword(s):  
Arterial Occlusion ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
The United States ◽  
Peripheral Blood Flow ◽  
Antithrombotic Agent ◽  
United States Food ◽  
States Food ◽  
Peripheral Arterial ◽  
Pde3 Inhibitor

IntroductionCilostazol, 6-[ 4-( l-cyclohexy l-IH-tetrazol-5yl ) butoxy ]-3, 4-dihydro-2 (1H)-quinolinone, is an antiplatelet/ antithrombotic agent that has been used since 1988 in several countries, including Japan, for treatment of chronic peripheral arterial occlusion. Cilostazol was also approved by the United States Food and Drug Administration (FDA) in 1999 for the treatment of intermittent claudication. Cilostazol not only inhibits platelet activation but also increases vasodilation.1,2 In addition, it has been also shown to inhibit vascular smooth muscle cell proliferation.3 These properties of cilostazol may also improve peripheral blood flow. The mechanism of these effects is mediated through elevated cyclic adenosine monophosphate (AMP), brought about by inhibition of cyclic nucleotide phosphodiesterase (PDE3) activity.4 In this chapter, the pharmacological properties, as well as the clinical efficacy of cilostazol, are briefly discussed.

Evaluation of an infrared laser-Doppler blood flowmeter

1987 ◽  
Vol 252 (6) ◽  
pp. G832-G839 ◽  
Author(s):  
A. P. Shepherd ◽  
G. L. Riedel ◽  
J. W. Kiel ◽  
D. J. Haumschild ◽  
L. C. Maxwell
Keyword(s):  
Rotational Velocity ◽  
Rotating Disk ◽  
Tissue Perfusion ◽  
Infrared Laser ◽  
Blood Oxygenation ◽  
Laser Doppler ◽  
Laser Doppler Velocimeter ◽  
Flow Signal

Several laser-Doppler blood flowmeters are now commercially available; however, only one utilizes an infrared laser diode (Laserflo, TSI, St. Paul, MN). Because of this and other unique features such as its microprocessor-based signal analyzer, we evaluated this device's ability to measure tissue perfusion. Initially, we determined whether laser illumination directly affected the microvasculature. Intravital microscopic observations in the hamster cremaster muscle indicated that neither He-Ne nor infrared laser light affected the diameters of arterioles that were responsive to vasoactive agents. To test the flowmeter for linearity and repeatability, we used a rotating disk to simulate a light-scattering, flowing medium. The "flow" signal was highly correlated (r = 0.99) with the rotational velocity of the disk, was consistent among flow probes, and showed a high degree of reproducibility. The second model consisted of microsphere suspensions pumped through cuvettes. The laser-Doppler velocimeter (LDV) flow signal was linear with respect to pump output. With red blood cells in the perfusate, we examined the effects of blood oxygenation on the flowmeter's performance. The LDV flow signal was unaffected by changes in blood oxygenation. We evaluated linearity in vivo in isolated, perfused rat livers and in isolated canine gastric flaps. We observed linear relationships between total flow and laser-Doppler flow measured on the surface of the liver (r = 0.98) and in the gastric mucosa (r = 0.98), but the slopes of the relationships between total and local LDV flow showed considerable variability not noted in the in vitro studies.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Recurrent Peripheral Arterial Occlusion in Acute Promyelocytic Leukemia

EJVES Extra ◽  
2003 ◽  
Vol 6 (5) ◽  
pp. 100-102 ◽  
Author(s):  
H. Posacioglu ◽  
A.Z. Apaydin ◽  
F. Buyukkececi ◽  
S. Soydan ◽  
İ. Durmaz
Keyword(s):  
Acute Promyelocytic Leukemia ◽  
Arterial Occlusion ◽  
Promyelocytic Leukemia ◽  
Peripheral Arterial ◽  
Peripheral Arterial Occlusion
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