scholarly journals A Novel Colorimetric Nano Aptasensor for Ultrasensitive Detection of Aflatoxin B1 Based on the Exonuclease III-Assisted Signal Amplification Approach

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2568
Author(s):  
Yu Chen ◽  
Fuyuan Zhang ◽  
Ruobing Liu ◽  
Minxuan Liu ◽  
Yaxin Sang ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Signal Amplification ◽  
Dynamic Range ◽  
Exonuclease Iii ◽  
Relative Standard ◽  
Cereal Samples ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Amplification Procedure ◽  
Better Than

The detection of aflatoxin B1 (AFB1) has recently garnered much attention on the issue of food safety. In this study, a novel and sensitive aptasensor towards AFB1 is proposed using an Exonuclease III (Exo III)-integrated signal amplification strategy. This reported sensing strategy is regulated by aptamer-functionalized nanobeads that can target AFB1; furthermore, complementary DNA (cDNA) strands can lock the immobilized aptamer strands, preventing the signal amplification function of Exo III in the absence of AFB1. The presence of AFB1 triggers the displacement of cDNA, which will then activate the Exo III-integrated signal amplification procedure, resulting in the generation of a guanine (G)-rich sequence to form a G-4/hemin DNAzyme, which can catalyze the substrate of ABTS to produce a green color. Using this method, a practical detection limit of 0.0032 ng/mL and a dynamic range of detection from 0.0032 to 50 ng/mL were obtained. Additionally, the practical application of the established sensing method for AFB1 in complex matrices was demonstrated through recovery experiments. The recovery rate and relative standard deviations (RSD) in three kinds of cereal samples ranged from 93.83% to 111.58%, and 0.82% to 7.20%, respectively, which were comparable with or better than previously reported methods.

scholarly journals Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy

Nanomaterials ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 104
Author(s):  
Fuyuan Zhang ◽  
Linyang Liu ◽  
Shengnan Ni ◽  
Jiankang Deng ◽  
Guo-Jun Liu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Aflatoxin M1 ◽  
Exonuclease Iii ◽  
Sensor Performance ◽  
Milk Samples ◽  
Increased Sensitivity ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Optimized Conditions ◽  
Magnetic Nanobeads

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.

Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86625-86630 ◽  
Author(s):  
Haiyan Zhao ◽  
Lei Wang ◽  
Xingti Liu ◽  
Zhiyue Gao ◽  
Wei Jiang
Keyword(s):  
Nicotinamide Adenine Dinucleotide ◽  
Signal Amplification ◽  
Nicotinamide Adenine ◽  
Label Free ◽  
Exonuclease Iii ◽  
Label Free Detection ◽  
Exo Iii ◽  
Amplification Strategy

Schematic illustration of the Exo III-assisted amplification strategy for NAD+ detection.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5670-5675 ◽  
Author(s):  
Qiong Li ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Zhi Liu ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Signal Amplification ◽  
Meca Gene ◽  
Exonuclease Iii ◽  
G Quadruplex ◽  
Exo Iii ◽  
Fluorescence Biosensor ◽  
Amplification Strategy

An ultrasensitive biosensor was developed for the detection of the mecA gene of Staphylococcus aureus based on the Exo III and G-quadruplex.

Multi-signal amplification electrochemical DNA biosensor based on exonuclease III and tetraferrocene

2020 ◽  
Vol 8 (18) ◽  
pp. 4143-4150
Author(s):  
Zhaojiang Yin ◽  
Hanfeng Cui ◽  
Qingxia Shu ◽  
Chen Jin ◽  
Yan Lin ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Electrochemical Sensor ◽  
Signal Amplification ◽  
Dna Biosensor ◽  
Exonuclease Iii ◽  
Electrochemical Dna Biosensor ◽  
Exo Iii

A new and efficient signal marker, 3,5-bis(3,5-bisferrocenethoxybenzyloxy)benzoic acid, was firstly synthesized and used for labeling. Construct a homogeneous electrochemical sensor with Exo III assisting the target cycle.

A cascade signal amplification strategy for sensitive and label-free DNA detection based on Exo III-catalyzed recycling coupled with rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2884-2889 ◽  
Author(s):  
Xingti Liu ◽  
Qingwang Xue ◽  
Yongshun Ding ◽  
Jing Zhu ◽  
Lei Wang ◽  
...  
Keyword(s):  
Detection Method ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Exonuclease Iii ◽  
Rolling Circle ◽  
Free Dna ◽  
Exo Iii ◽  
Amplification Strategy ◽  
Cascade Amplification

A sensitive and label-free DNA detection method was developed based on cascade amplification combining exonuclease-III recycling with rolling circle amplification.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

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