scholarly journals Effects of Medium Composition and Genetic Background on Agrobacterium-Mediated Transformation Efficiency of Lentinula edodes

Genes ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 467 ◽  
Author(s):  
Lianlian Yan ◽  
Ruiping Xu ◽  
Yan Zhou ◽  
Yuhua Gong ◽  
Shenghong Dai ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Lentinula Edodes ◽  
Transformation Efficiency ◽  
Edible Mushroom ◽  
Transformation Method ◽  
Medium Composition ◽  
Agrobacterium Mediated Transformation ◽  
Genomics Research ◽  
Wild Strains ◽  
Positive Frequency

The establishment of genetic transformation method is crucial for the functional genomics research in filamentous fungi. Although the transformation method has been developed in several types of fungi, a highly efficient and convenient transformation system is desperately needed in Lentinula edodes. Present work established the Agrobacterium-mediated transformation (ATMT) of basidiomycete L. edodes in both monokaryon and dikaryon mycelia by using constructed binary plasmid pCAMBIA-1300-GFP. Then, the transformation efficiency of ATMT was evaluated by using different mediums for recipient incubation and different varieties of L. edodes. The results showed that in dikaryon strain W1, the positive hygromycin-resistant transformants was observed in all medium with the positive frequency of selected transformants that ranged from 0 to 30%. While in the monokaryon strain W1-26, only the millet medium group obtained positive transformants with a positive frequency of 75.48%. Moreover, three dikaryotic wild strains (YS55, YS3334, and YS3357) and two dikaryotic cultivated strains (W1 and S606) showed the highest transformation efficiency, with 32.96% of the germination frequency, and 85.12% of positive frequency for hygromycin-resistant transformants. This work demonstrated that Agrobacterium-mediated transformation was successfully performed in L. edodes, and the genotype of recipients as well as the medium for mycelial incubation were suggested to play key roles in determining the transformation efficiency. These findings may provide new avenues for the genetic modification of edible mushroom and may extend the cognition of DNA-mediated transformation in filamentous fungi.

scholarly journals An efficient Agrobacterium-mediated transformation method for the edible mushroom Hypsizygus marmoreus

2014 ◽  
Vol 169 (9-10) ◽  
pp. 741-748 ◽  
Author(s):  
Jin jing Zhang ◽  
Liang Shi ◽  
Hui Chen ◽  
Yun qi Sun ◽  
Ming wen Zhao ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Transformation Method ◽  
Hypsizygus Marmoreus ◽  
Agrobacterium Mediated Transformation

scholarly journals Biosurfactants from Trichoderma Filamentous Fungi—A Preliminary Study

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 519
Author(s):  
Michał Piegza ◽  
Joanna Pietrzykowska ◽  
Joanna Trojan-Piegza ◽  
Wojciech Łaba
Keyword(s):  
Filamentous Fungi ◽  
Chemical Nature ◽  
Culture Fluid ◽  
Medium Composition ◽  
Organic Media ◽  
Plant Leaves ◽  
Specific Behavior ◽  
Extracellular Secretion ◽  
Trichoderma Fungi ◽  
Preliminary Study

Biosurfactants represent a structurally diverse group of secondary metabolites produced by bacteria, yeasts, and filamentous fungi. Their character is often associated with numerous additional properties. The observation of Trichoderma fungi of various species used as a source of bioinhibitors against pathogenic plants fungi focuses attention to the often quite specific behavior of preparations in contact with, among others, plant leaves, dependent on strain. Thus, an evaluation of the selected strains belonging to the species: T. atroviride, T. citrinoviride,T. reesei and T. harzianum was conducted towards their capability of the extracellular secretion of surfactants, with a simultaneous attempt to pre-determine their chemical nature. Two mineral-organic media were used for this purpose, and the culture fluid was extensively tested using a variety of methods. A decrease in surface tension was observed in culture fluid of each tested strain, especially T. citrinoviride HL and C1. The results strongly depended on medium composition, of which Saunders 1 and MGP 1 were most beneficial. The secreted compounds were further analyzed to pre-determine their chemical nature using IR, GC, and NMR. In the case of most efficient biosurfactant producers, a lipopeptide structure of the surfactants was concluded.

scholarly journals Biological and Molecular Characteristics of a Novel Partitivirus Infecting the Edible Fungus Lentinula edodes

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 726-733 ◽  
Author(s):  
Mengpei Guo ◽  
Yinbing Bian ◽  
Jinjie Wang ◽  
Gangzheng Wang ◽  
Xiaolong Ma ◽  
...  
Keyword(s):  
Lentinula Edodes ◽  
Control Strategies ◽  
Phylogenetic Analyses ◽  
Pcr Analysis ◽  
Molecular Characteristics ◽  
Rt Pcr ◽  
Edible Fungus ◽  
Qpcr Analysis ◽  
Disease Diagnostics ◽  
Wild Strains

A new partitivirus named Lentinula edodes partitivirus 1 (LePV1) was isolated from a diseased L. edodes strain with severe degeneration of the mycelium and imperfect browning in bag cultures. The nucleotide sequences of LePV1 dsRNA-1 and dsRNA-2 were determined; they were 2,382 bp and 2,245 bp in length, and each contained a single ORF encoding RNA-dependent RNA polymerase (RdRp) and coat protein (CP), respectively. The purified virus preparation contained isometric particles 34 nm in diameter encapsidating these dsRNAs. Phylogenetic analyses showed LePV1 to be a new member of Betapartitivirus, with the RdRp sequence most closely related to Grapevine partitivirus. RT-PCR analysis showed that 27 of the 56 Chinese L. edodes core collection strains carry LePV1, with the virus being more common in wild strains than cultivated strains. In addition, qPCR analysis suggested that coinfection with L. edodes mycovirus HKB (LeV-HKB) could increase replication of the RdRp gene of LePV1. This study may be essential for the development of more accurate disease diagnostics and the formulation of control strategies for viral diseases in L. edodes.

scholarly journals Analysis of Genetic Diversity and Population Structure of Wild Strains and Cultivars Using Genomic SSR Markers in Lentinula edodes

Mycobiology ◽  
2020 ◽  
Vol 48 (2) ◽  
pp. 115-121
Author(s):  
Hwa-Yong Lee ◽  
Suyun Moon ◽  
Hyeon-Su Ro ◽  
Jong-Wook Chung ◽  
Hojin Ryu
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers ◽  
Lentinula Edodes ◽  
Genomic Ssr ◽  
Wild Strains

Transformation of Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 by CaCl2 method

2010 ◽  
Vol 56 (1) ◽  
pp. 74-76 ◽  
Author(s):  
Urmi Patel ◽  
Sarika Sinha
Keyword(s):  
Escherichia Coli ◽  
Optical Density ◽  
Sinorhizobium Meliloti ◽  
Transformation Efficiency ◽  
Binary Vector ◽  
Transformation Method ◽  
Mesorhizobium Ciceri ◽  
Competent Cells

The CaCl2 method, commonly used for transformation of Escherichia coli, was modified and used to develop a simpler and easier transformation method for Rhizobia sp. Two species of Rhizobia, Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620, were transformed with the 13.2 kb binary vector pGA482. At an optical density of 0.4, the transformation efficiencies in Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 were 104 and 103, respectively. Competent cells of Sinorhizobium meliloti MTCC 100 were prepared at different growth intervals and transformed by the same vector. A maximum transformation efficiency of 104 was achieved at an optical density of 0.5.

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