scholarly journals Genotoxic Effects of Tributyltin and Triphenyltin Isothiocyanates, Cognate RXR Ligands: Comparison in Human Breast Carcinoma MCF 7 and MDA-MB-231 Cells

2019 ◽  
Vol 20 (5) ◽  
pp. 1198 ◽  
Author(s):  
Luba Hunakova ◽  
Eva Horvathova ◽  
Karolina Majerova ◽  
Pavel Bobal ◽  
Jan Otevrel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Human Breast Carcinoma ◽  
Human Breast ◽  
Receptor Ligands ◽  
Estrogen Receptor Positive ◽  
Breast Carcinoma Cell Lines ◽  
Mcf 7

The cytotoxicity of two recently synthesized triorganotin isothiocyanate derivatives, nuclear retinoid X receptor ligands, was tested and compared in estrogen-receptor-positive MCF 7 and -negative MDA-MB-231 human breast carcinoma cell lines. A 48 h MTT assay indicated that tributyltin isothiocyanate (TBT-ITC) is more cytotoxic than triphenyltin isothiocyanate (TPT-ITC) in MCF 7 cells, and the same trend was observed in the MDA-MB-231 cell line. A comet assay revealed the presence of both crosslinks and increasing DNA damage levels after the 17 h treatment with both derivatives. Differences in cytotoxicity of TBT-ITC and TPT-ITC detected by FDA staining correspond to the MTT data, communicating more pronounced effects in MCF 7 than in the MDA-MB-231 cell line. Both derivatives were found to cause apoptosis, as shown by the mitochondrial membrane potential (MMP) depolarization and caspase-3/7 activation. The onset of caspase activation correlated with MMP dissipation and the total cytotoxicity more than with the amount of active caspases. In conclusion, our data suggest that the DNA damage induced by TBT-ITC and TPT-ITC treatment could underlie their cytotoxicity in the cell lines studied.

The apoptotic effects of silibinin on MDA-MB-231 and MCF-7 human breast carcinoma cells

2016 ◽  
Vol 36 (6) ◽  
pp. 573-586 ◽  
Author(s):  
D Bayram ◽  
ES Çetin ◽  
M Kara ◽  
M Özgöçmen ◽  
IA Candan
Keyword(s):  
Breast Carcinoma ◽  
Cell Lines ◽  
Human Breast Carcinoma ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Dead Cell ◽  
Human Breast ◽  
Human Breast Carcinoma Cells ◽  
Breast Carcinoma Cell Lines ◽  
Mcf 7

Background: Silibinin is a bioactive flavonolignan extracted from milk thistle, known as Silybum marianum. Silibinin exerts strong antiproliferative, proapoptotic, and anti-inflammatory effects. Many studies have shown that silibinin inhibits experimentally induced malignancies of the liver, prostate, skin, and colon as well as promotes inhibition of the proliferation of cancer cell lines in vitro. This study aimed to investigate the effects of silibinin on the human breast carcinoma cell lines MDA-MB-231 and MCF-7 in monolayer and spheroid cultures. Method: The MDA-MB-231 and MCF-7 cell lines were cultured in both monolayer and spheroid cultures. Cells were treated with silibinin at 24, 48, and 72 h of incubation. The 5-bromo-2′-deoxyuridine labeling index was used to determine the cells of the synthesis phase. Poly-ADP-ribose-polimerase immunohistochemical staining and the terminal deoxynucleotidyl transferase dUTP nick and labeling assay were used to determine the death of cells in both the monolayer and spheroid cultures. Results: An half maximal inhibitory concentration dose of silibinin in MDA-MB-231 and MCF-7 cells was 100 µM/mL at 24, 48, and 72 h of incubation. Terminal deoxynucleotidyl transferase dUTP nick and labeling positive cells and active poly-ADP-ribose-polimerase were detected after treatment with silibinin in both the monolayer and spheroid cultures. The dead cell count was higher in the MDA-MB-231 and MCF-7 cell lines with silibinin applied than in the controls. Conclusions: Our study demonstrated that silibinin applications enhanced terminal deoxynucleotidyl transferase dUTP nick and labeling positive cells and active poly-ADP-ribose-polimerase in comparison to the control in both the monolayer and spheroid cultures.

Replication of oncornavirus-like particle in human breast carcinoma cell line, MCF-7

Nature ◽  
1974 ◽  
Vol 252 (5480) ◽  
pp. 247-250 ◽  
Author(s):  
CHARLES M. MCGRATH ◽  
PETER M. GRANT ◽  
HERBERT D. SOULE ◽  
TERRY GLANCY ◽  
MARVIN A. RICH
Keyword(s):  
Breast Carcinoma ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Human Breast Carcinoma ◽  
Carcinoma Cell Line ◽  
Breast Carcinoma Cell Line ◽  
Human Breast ◽  
Human Breast Carcinoma Cell ◽  
Mcf 7

Antiproliferative activity of nanofibers containing quaternized chitosan and/or doxorubicin against MCF-7 human breast carcinoma cell line by apoptosis

2011 ◽  
Vol 26 (6) ◽  
pp. 539-551 ◽  
Author(s):  
Milena Ignatova ◽  
Lilia Yossifova ◽  
Elena Gardeva ◽  
Nevena Manolova ◽  
Reneta Toshkova ◽  
...  
Keyword(s):  
Breast Carcinoma ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
Carcinoma Cell ◽  
Human Breast Carcinoma ◽  
Breast Carcinoma Cell Line ◽  
Human Breast ◽  
Human Breast Carcinoma Cell ◽  
Quaternized Chitosan ◽  
Mcf 7

The antiproliferative activity of electrospun mats of poly(L-lactide- co-D,L-lactide) (coPLA) containing quaternized chitosan (QCh) and/or doxorubicin hydrochloride (DOX) was evaluated against the Michigan Cancer Foundation-7(MCF-7) human breast carcinoma cell line. QCh- and DOX-containing nanofibrous mats possess good antiproliferative activity and decrease considerably the viability of the MCF-7 cells for the different periods of cell incubation as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Fluorescent microscopy analyses and scanning electron microscopy observations revealed that apoptosis was one of the major mechanisms of MCF-7 cell death induced by the QCh- and DOX-containing mats.

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