scholarly journals Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides

2021 ◽  
Vol 22 (11) ◽  
pp. 5641
Author(s):  
Anastasiya A. Kasimova ◽  
Nikolay P. Arbatsky ◽  
Jacob Tickner ◽  
Johanna J. Kenyon ◽  
Ruth M. Hall ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Capsular Polysaccharide ◽  
Gene Clusters ◽  
13C Nmr Spectroscopy ◽  
Side Chain ◽  
Capsular Polysaccharides ◽  
Two Dimensional ◽  
Genome Sequences ◽  
1H And 13C Nmr ◽  
Biosynthesis Gene

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.

Updated analysis of the surface carbohydrate gene clusters in the diverse panel of Acinetobacter baumannii isolates.

2021 ◽  
Author(s):  
Johanna J Kenyon ◽  
Ruth M. Hall
Keyword(s):  
Acinetobacter Baumannii ◽  
Capsular Polysaccharide ◽  
Gene Clusters ◽  
Outer Core ◽  
The Novel

To enhance the utility of the genetically diverse panel of Acinetobacter baumannii isolates reported recently by Galac and co-workers (AAC 64: e00840-20) and to identify the novel KL and OCL, all of the gene clusters that direct the biosynthesis of capsular polysaccharide and of the outer core of lipooligosaccharide, respectively, were re-examined. The nine KL and one OCL previously recorded as novel were identified and nine further novel KL and two OCL were found.

Identification of Aminopyrimidine Regioisomers via Line Broadening Effects in 1H and 13C NMR Spectroscopy

2004 ◽  
Vol 57 (11) ◽  
pp. 1079 ◽  
Author(s):  
James Garner ◽  
Tim Hill ◽  
Luke Odell ◽  
Paul Keller ◽  
Jody Morgan ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Diagnostic Tool ◽  
Room Temperature ◽  
Medicinal Chemistry ◽  
13C Nmr Spectroscopy ◽  
Primary Amines ◽  
Two Dimensional ◽  
Line Broadening ◽  
1H And 13C Nmr

Substituted mono- and diamino-pyrimidines were synthesized as part of our medicinal chemistry programmes. Primary amines substituted at the 4-position exhibited room-temperature line broadening effects in both 1H and 13C NMR spectroscopy due to the presence of rotamers, but these effects were not observed for substituents in the 2-position. This provided a simple diagnostic tool for the identification of regioisomers, a determination which would otherwise have required two-dimensional experiments.

scholarly journals The K46 and K5 capsular polysaccharides produced by Acinetobacter baumannii NIPH 329 and SDF have related structures and the side-chain non-ulosonic acids are 4-O-acetylated by phage-encoded O-acetyltransferases

PLoS ONE ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. e0218461 ◽  
Author(s):  
Johanna J. Kenyon ◽  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Anastasiya V. Popova ◽  
Andrei S. Dmitrenok ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Side Chain ◽  
Capsular Polysaccharides

scholarly journals antiSMASH: rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences

2011 ◽  
Vol 39 (suppl_2) ◽  
pp. W339-W346 ◽  
Author(s):  
Marnix H. Medema ◽  
Kai Blin ◽  
Peter Cimermancic ◽  
Victor de Jager ◽  
Piotr Zakrzewski ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Gene Clusters ◽  
Rapid Identification ◽  
Fungal Genome ◽  
Genome Sequences ◽  
Biosynthesis Gene ◽  
Secondary Metabolite Biosynthesis

Related structures of neutral capsular polysaccharides of Acinetobacter baumannii isolates that carry related capsule gene clusters KL43, KL47, and KL88

2016 ◽  
Vol 435 ◽  
pp. 173-179 ◽  
Author(s):  
Alexander S. Shashkov ◽  
Johanna J. Kenyon ◽  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Anastasiya V. Popova ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Gene Clusters ◽  
Capsular Polysaccharides

Chemistry of chromotropic acid. 1H and 13C NMR spectroscopy of chromotropic acid and its derivatives

1991 ◽  
Vol 69 (8) ◽  
pp. 1207-1211 ◽  
Author(s):  
Paris E. Georghiou ◽  
Chi Keung (Jimmy) Ho ◽  
Chester R. Jablonski
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Nmr Spectra ◽  
Deuterium Oxide ◽  
13C Nmr Spectroscopy ◽  
Chromotropic Acid ◽  
Hydroxyl Groups ◽  
Two Dimensional ◽  
1H And 13C Nmr ◽  
C Nmr

The 1H and 13C NMR spectra of chromotropic acid (CTA) (4,5-dihydroxy-2,7-naphthalenedisulphonic acid) have been unambiguously assigned. Proton NOED spectra were used to show the proximity of both H-3 and H-6 and the hydroxyl groups. Two-dimensional 1H–13C NMR correlation spectra of CTA, of its corresponding diacetoxy derivative, and of 3-bromo- and 3,6-dibromo-CTA support the assignments. A regioselective deuterium exchange reaction of the C-3 and C-6 protons of CTA with deuterium oxide was observed during the NMR experiments. This latter finding is strongly indicative of the mode of formation, and of the nature of the chromogen formed in the reaction of CTA with formaldehyde in the well-known CTA-formaldehyde analytical reaction. Key words: chromotropic acid, 3-bromochromotropic acid, 3,6-dibromochromotropic acid.

scholarly journals Synthesis, structural and conformational study of selected N-substituted phthalimides

2005 ◽  
Vol 3 (4) ◽  
pp. 683-704 ◽  
Author(s):  
Isabel Iriepa ◽  
F. Javier Villasante ◽  
Enrique Gálvez ◽  
Antonio Herrera ◽  
Angel Sánchez ◽  
...  
Keyword(s):  
Double Resonance ◽  
13C Nmr Spectroscopy ◽  
Two Dimensional ◽  
X Ray Diffraction ◽  
1H And 13C Nmr ◽  
Conformational Studies ◽  
One Dimensional ◽  
X Ray ◽  
Nmr Techniques ◽  
New Compounds

AbstractThis paper synthesizes N-substituted phthalimides derived from nitrogen heterocycles as potential 5-HT4 ligands by using the Mitsunobu reaction. Conformational studies of some of the new compounds have been conducted using 1H and 13C-NMR spectroscopy. Proton and carbon resonances were achieved through the application of one-dimensional selective NOE, two-dimensional NMR techniques-homonuclear COSY-45, NOESY and heteronuclear 1H-13C HMQC correlated spectroscopy- and double resonance experiments. The crystal structure of compound 1 was determined by X-ray diffraction.

Structures of three different neutral polysaccharides of Acinetobacter baumannii, NIPH190, NIPH201, and NIPH615, assigned to K30, K45, and K48 capsule types, respectively, based on capsule biosynthesis gene clusters

2015 ◽  
Vol 417 ◽  
pp. 81-88 ◽  
Author(s):  
Alexander S. Shashkov ◽  
Johanna J. Kenyon ◽  
Nikolay P. Arbatsky ◽  
Mikhail M. Shneider ◽  
Anastasiya V. Popova ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Gene Clusters ◽  
Biosynthesis Gene
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