scholarly journals Photodynamic Inactivation of an Endodontic Bacteria Using Diode Laser and Indocyanine Green-Loaded Nanosphere

2021 ◽  
Vol 22 (16) ◽  
pp. 8384
Author(s):  
Naoya Higuchi ◽  
Jun-ichiro Hayashi ◽  
Masanori Fujita ◽  
Yuki Iwamura ◽  
Yasuyuki Sasaki ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Diode Laser ◽  
Root Canal ◽  
High Efficiency ◽  
Inflammatory Lesion ◽  
Bactericidal Effect ◽  
Apical Periodontitis ◽  
High Recurrence Rate ◽  
Log Reduction ◽  
Infected Root

Apical periodontitis, an inflammatory lesion causing bone resorption around the apex of teeth, is treated by eradicating infectious bacteria from the root canal. However, it has a high recurrence rate and often requires retreatment. We investigated the bactericidal effect of antimicrobial photodynamic therapy (aPDT)/photodynamic antimicrobial chemotherapy (PACT) using indocyanine green (ICG)-loaded nanospheres coated with chitosan and a diode laser on a biofilm of Enterococcus faecalis, a pathogen of refractory apical periodontitis. Biofilm of E. faecalis was cultured in a porcine infected root canal model. ICG solution was injected into the root canal, which was then irradiated with a laser (810 nm wavelength) from outside the root canal. The bactericidal effect was evaluated by colony counts and scanning electron microscopy. The result of the colony counts showed a maximum 1.89 log reduction after irradiation at 2.1 W for 5 min. The temperature rise during aPDT/PACT was confirmed to be within a safe range. Furthermore, the light energy transmittance through the root was at a peak approximately 1 min after the start of irradiation, indicating that most of the ICG in the root canal was consumed. This study shows that aPDT/PACT can suppress E. faecalis in infected root canals with high efficiency.

Diode Laser Radiation and Its Bactericidal Effect in Root Canal Wall Dentin

2000 ◽  
Vol 18 (2) ◽  
pp. 57-60 ◽  
Author(s):  
NORBERT GUTKNECHT ◽  
DIRK VAN GOGSWAARDT ◽  
GEORG CONRADS ◽  
CHRISTIAN APEL ◽  
CLAUS SCHUBERT ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Diode Laser ◽  
Root Canal ◽  
Bactericidal Effect ◽  
Canal Wall

scholarly journals Effect of a 980-nm diode laser on post-operative pain after endodontic treatment in teeth with apical periodontitis: A randomized clinical trial

2020 ◽  
Author(s):  
Tuna Kaplan ◽  
Güzide Pelin Sezgin ◽  
Sema Sönmez Kaplan
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Control Group ◽  
Pain Level ◽  
Post Operative Pain ◽  
Average Pain ◽  
Laser Group

Abstract Background: This study aimed to assess the effect of a 980-nm diode laser following chemomechanical root canal preparation on the severity of post-operative pain (PP) after root canal treatment (RCT).Methods: This study included asymptomatic, single-rooted teeth with a periapical index (PAI) score of 3 or 4. All the patients received RCT, including dressing with calcium hydroxide (Ca(OH)2), during two visits. The patients were randomly divided into two groups (n:30): a control group, in which the final irrigation was performed using 5 ml of 2.5% sodium hypochlorite (NaOCI), followed by 5 ml of 17% and ethylenediaminetetraacetic acid (EDTA) and 5 ml of distilled water, and a laser group, in which the root canals were irradiated using a 980-nm diode laser after the final irrigation at both visits. The pain levels after both visits were evaluated using a visual analogue scale (VAS) after 8 h, 24 h, 48 h and 7 d. In addition, the time intervals to medication intake were recorded. The collected data were statistically analysed using the chi-square and Mann–Whitney U test (p<0.05).Results: The average pain level in the control group 24 h after the first visit was significantly higher than that in the laser group (p<0.05). The average pain level 24 h and 48 h after the second visit was significantly higher in the control group (p<0.05). The levels of PP 24 h after the first visit were higher than those after the second visit only in the control group (p<0.05). After the first visit, analgesic use in the control group was significantly higher after 8 h (40%) and 24 h (23%) as compared with that in the laser group (p<0.05).Conclusions: Root canal irradiation with a diode laser may reduce PP after RCT in single-rooted teeth with a PAI score of 3 or 4.Trial registration: Effect of the Diode Laser on Post-operative Pain After Endodontic Treatment in Teeth with Apical Periodontitis: NCT04486196. Registered 24 July 2020 - Retrospectively registered, http://clinicaltrials.gov/ct2/show/NCT04486196

Bactericidal effect of 445-nm blue diode laser in the root canal dentin on Enterococcus faecalis of human teeth

2018 ◽  
Vol 2 (4) ◽  
pp. 247-254 ◽  
Author(s):  
Norbert Gutknecht ◽  
Nour Al Hassan ◽  
Miguel R. Martins ◽  
Georg Conrads ◽  
Rene Franzen
Keyword(s):  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bactericidal Effect ◽  
Human Teeth ◽  
Root Canal Dentin ◽  
Blue Diode Laser

scholarly journals Effect of a 980-nm diode laser on post-operative pain after endodontic treatment in teeth with apical periodontitis: a randomized clinical trial

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tuna Kaplan ◽  
Güzide Pelin Sezgin ◽  
Sema Sönmez Kaplan
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Control Group ◽  
Pain Level ◽  
Post Operative Pain ◽  
Average Pain ◽  
Laser Group

Abstract Background This study aimed to assess the effect of a 980-nm diode laser following chemomechanical root canal preparation on the severity of post-operative pain (PP) after root canal treatment (RCT). Methods This study included asymptomatic, single-rooted teeth with a periapical index (PAI) score of 3 or 4. All the patients received RCT, including dressing with calcium hydroxide (Ca(OH)2), during two visits. The patients were randomly divided into two groups (n:30): a control group, in which the final irrigation was performed using 5 ml of 2.5% sodium hypochlorite (NaOCI), followed by 5 ml of 17% and ethylenediaminetetraacetic acid (EDTA) and 5 ml of distilled water, and a laser group, in which the root canals were irradiated using a 980-nm diode laser after the final irrigation at both visits. The pain levels after both visits were evaluated using a visual analogue scale (VAS) after 8 h, 24 h, 48 h and 7 d. In addition, the time intervals to medication intake were recorded. The collected data were statistically analysed using the chi-square and Mann–Whitney U test (p < 0.05). Results The average pain level in the control group 24 h after the first visit was significantly higher than that in the laser group (p < 0.05). The average pain level 24 h and 48 h after the second visit was significantly higher in the control group (p < 0.05). The levels of PP 24 h after the first visit were higher than those after the second visit only in the control group (p < 0.05). After the first visit, analgesic use in the control group was significantly higher after 8 h (40%) and 24 h (23%) as compared with that in the laser group (p < 0.05). Conclusions Root canal irradiation with a diode laser may reduce PP after RCT in single-rooted teeth with a PAI score of 3 or 4. Trial registration: Effect of the Diode Laser on Post-operative Pain After Endodontic Treatment in Teeth with Apical Periodontitis: NCT04486196. Registered 24 July 2020—Retrospectively registered, http://clinicaltrials.gov/ct2/show/NCT04486196

Bactericidal Effect of a 980-nm Diode Laser in the Root Canal Wall Dentin of Bovine Teeth

2004 ◽  
Vol 22 (1) ◽  
pp. 9-13 ◽  
Author(s):  
N. Gutknecht ◽  
R. Franzen ◽  
M. Schippers ◽  
F. Lampert
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Bactericidal Effect ◽  
Canal Wall

scholarly journals Effect of Root Canal Disinfection with 980 µm Diode Laser on Post-operative Pain after Endodontic Treatment in Teeth with Apical Periodontitis: A Randomized Clinical Trial

2020 ◽  
Author(s):  
Tuna Kaplan ◽  
Güzide Pelin Sezgin ◽  
Sema Sönmez Kaplan
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Root Canal Treatment ◽  
Control Group ◽  
Pain Level ◽  
Root Canal Disinfection ◽  
Post Operative Pain ◽  
Average Pain

Abstract Background: This study aims to assess the effect of root canal disinfection with a 980 µm diode laser following chemomechanical root canal preparation on the severity of pain after root canal treatment (RCT).Methods: In present study, asymptomatic, single-rooted teeth with periapical index (PAI) score 3 or 4 were included. All patients were treated with two visits of root canal treatment including dressing with calcium hydroxide. Patients were randomly divided into two groups (n: 28). ‘Control (no laser)’: The final irrigation was performed using 5ml 2.5% NaOCI, followed by 5 ml 17% EDTA and 5 ml distilled water. ‘Laser Disinfection (LD)’: Root canals were irradiated with 980 µm diode laser after final irrigation at both visits. The pain levels were evaluated using visual analog scale (VAS) after 8, 24, 48 hours and 7 days. In addition, analgesic intake and time intervals were recorded by patients. The collected data were statistically analyzed with the Chi-square and Mann-Whitney U test (p<0.05).Results: The average pain level in the control group after 24 hours was significantly higher than that in the laser group after the first appointment (p<0.05). The average pain level after the second appointment was statistically higher at 24 h and 48 h in the control group (p<0.05). PP levels at 24-hs after the first appointment were higher than the second appointment only in the control group (p<0.05). After first appointment, analgesic use in the control group at 8 h (40%) and 24 h (23%) were significantly higher than LD group (p<0.05).Conclusions: Root canal disinfection with diode laser may reduce PP in single-rooted teeth with PAI score 3 or 4.Trial registration: Effect of the Diode Laser on Post-operative Pain After Endodontic Treatment in Teeth with Apical Periodontitis: NCT04486196. Registered 24 July 2020 - Retrospectively registered, http://clinicaltrials.gov/ct2/show/NCT04486196Clinical Trials ID: NCT04486196

scholarly journals COMPARATIVE EFFECTIVENESS OF TREATMENT OF CHRONIC APICAL PERIODONTITIS ON THE BASIS OF DENSITOMETRIC AND MICROBIOLOGICAL RESEARCH METHODS

2019 ◽  
Vol 15 (2) ◽  
pp. 17-24 ◽  
Author(s):  
Лариса Герасимова ◽  
Larisa Gerasimova ◽  
Алина Юсупова ◽  
Alina Yusupova ◽  
Ирина Усманова ◽  
...  
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Laboratory Data ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Canal System ◽  
Periodontal Tissues ◽  
Complex Anatomy ◽  
Group I ◽  
Root Canal System

Relevanct of the research topic. One of the possible causes of apical periodontitis is the infectious inflammatory process of the periapical periodontal tissues. The microflora of the root canal system is represented by various microorganisms, among which streptococci, enterococci and staphylococci most often prevail. The complex anatomy of the root canal system ensures the growth and reproduction of microorganisms, and the endodontic treatment does not always contribute to their qualitative and quantitative reduction. Purpose ― study of the effectiveness of endodontic treatment of chronic apical periodontitis in the near and long term. Methods. We observed 110 patients aged 20 to 30 years with apical periodontitis, bone density analysis and microbiological examination of the contents of the root canal. Depending on the obtained clinical and laboratory data, the patients were divided into two clinical groups: in group I endodontic treatment of 55 teeth was carried out according to the scheme developed by us; in group II — 55 teeth using the standard method of treatment. Results. In endodontic treatment of chronic apical periodontitis, treatment of the root canal system with a diode laser promotes better decontamination of the microflora. Coal mining introduction into the root canal system and in the region of the transition folds of the platelet autoplasma leads to an increase of the reparative process in the periapical region (p≤0.05). Summary. The inclusion in the Protocol of endodontic treatment of apical periodontitis of treatment of the root canal system with a diode laser and injection of platelet autoplasm is a more effective method of treatment in comparison with the traditional one.

scholarly journals MONITORING OF THE MICROCIRCULATION OF THE MUCOUS MEMBRANE CONDITION IN THE TRANSITORY FOLD OF TEETH WITH CHRONIC APICAL PERIODONTITIS IN THE DYNAMICS OF COMPLEX ENDODONTIC TREATMENT

2019 ◽  
Vol 15 (3) ◽  
pp. 75-81 ◽  
Author(s):  
Алина Юсупова ◽  
Alina Yusupova ◽  
Лариса Герасимова ◽  
Larisa Gerasimova ◽  
Ирина Усманова ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Laser Doppler Flowmetry ◽  
Root Canal ◽  
High Efficiency ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Laser Doppler ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Periodontal Tissues

Subject. A sufficiently sensitive, high-resolution method of laser doppler flowmetry is used to characterize the сlaudication in the oral mucous membrane and periodontal tissues. This method makes it possible to detect early changes in the microcirculatory bloodstream and predict the course of chronic apical periodontitis. With the help of the method of laser doppler fluorometry, a comparative monitoring of the microcirculatory bloodstream of the mucous membrane of the transitory fold in the area of intact teeth and teeth with chronic apical periodontal was carried out. The aim of this study was efficiency determination of complex endodontic treatment of chronic apical periodontitis on the basis of monitoring the state of microcirculation of the mucous membrane in the transitory fold of the studied teeth. Methodology. Control group consisted of 55 patients with chronic apical periodontitis, and 20 patients with intact teeth aged 20 to 30 years old who underwent the analysis of microcirculation of the mucous membrane of the transitional fold of the studied teeth using laser doppler flowmetry (LDF). Results. The indicators IM, Kv, Q among 20 patients with intact teeth examined with the method of laser doppler flowmetry (LDF) were taken as normal. Studied parameters are significantly lower than normal in 55 patients with chronic apical periodontitis. In case of patients with chronic apical periodontitis, the inclusion of complex composed of the root canal system treatment by diode laser and the insertion into the root canal trombocytic autoplasma and its injection in projection of the root end in the protocol of endodontic treatment of chronic apical periodontitis helps to normalize microcirculation in the mucosa of the transitory folds of the investigated teeth. Summary. The study of the dynamics of the complex of endodontic treatment of chronic apical periodontitis showed its high efficiency (p≤0.001).

Yeasts in Apical Periodontitis

2003 ◽  
Vol 14 (2) ◽  
pp. 128-137 ◽  
Author(s):  
T.M.T. Waltimo ◽  
B.H. Sen ◽  
J.H. Meurman ◽  
D. Ørstavik ◽  
M.P.P. Haapasalo
Keyword(s):  
Root Canal ◽  
Antimicrobial Agents ◽  
Root Apex ◽  
Apical Periodontitis ◽  
Dentinal Tubules ◽  
Root Canals ◽  
Infected Root ◽  
Pure Cultures ◽  
Oral Candida ◽  
Almost All

Microbiological reports of apical periodontitis have revealed that yeasts can be isolated from approximately 5-20% of infected root canals. They occur either in pure cultures or together with bacteria. Almost all isolated yeasts belong to the genus Candida, and the predominant species is C. albicans. Pheno- and genotypic profiles of C. albicans isolates show heterogeneity comparable with those of isolates from other oral sites. C. albicans expresses several virulence factors that are capable of infecting the dentin-pulp complex, including dentinal tubules. This causes, consequentially, an inflammatory response around the root apex, which suggests a pathogenic role for this organism in apical periodontitis. Yeasts are particularly associated with persistent root canal infections that do not respond favorably to conservative root canal therapy. This may be due to the resistance of all oral Candida species against a commonly used topical medicament, calcium hydroxide. However, other antimicrobial agents may offer alternative therapeutic approaches and improve the treatment of these persistent cases of apical periodontitis.

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