scholarly journals Oligomeric and Fibrillar Species of Aβ42 Diversely Affect Human Neural Stem Cells

2021 ◽  
Vol 22 (17) ◽  
pp. 9537
Author(s):  
Adela Bernabeu-Zornoza ◽  
Raquel Coronel ◽  
Charlotte Palmer ◽  
Victoria López-Alonso ◽  
Isabel Liste

Amyloid-β 42 peptide (Aβ1-42 (Aβ42)) is well-known for its involvement in the development of Alzheimer’s disease (AD). Aβ42 accumulates and aggregates in fibers that precipitate in the form of plaques in the brain causing toxicity; however, like other forms of Aβ peptide, the role of these peptides remains unclear. Here we analyze and compare the effects of oligomeric and fibrillary Aβ42 peptide on the biology (cell death, proliferative rate, and cell fate specification) of differentiating human neural stem cells (hNS1 cell line). By using the hNS1 cells we found that, at high concentrations, oligomeric and fibrillary Aβ42 peptides provoke apoptotic cellular death and damage of DNA in these cells, but Aβ42 fibrils have the strongest effect. The data also show that both oligomeric and fibrillar Aβ42 peptides decrease cellular proliferation but Aβ42 oligomers have the greatest effect. Finally, both, oligomers and fibrils favor gliogenesis and neurogenesis in hNS1 cells, although, in this case, the effect is more prominent in oligomers. All together the findings of this study may contribute to a better understanding of the molecular mechanisms involved in the pathology of AD and to the development of human neural stem cell-based therapies for AD treatment.

2010 ◽  
Vol 80 ◽  
pp. S33-S34
Author(s):  
J. Jeon ◽  
S. Cho ◽  
K. Cho ◽  
Y. Lee ◽  
M. Lee

2018 ◽  
Vol 56 (6) ◽  
pp. 4023-4036 ◽  
Author(s):  
A. Bernabeu-Zornoza ◽  
R. Coronel ◽  
C. Palmer ◽  
M. Calero ◽  
A. Martínez-Serrano ◽  
...  

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23414 ◽  
Author(s):  
Luis Ojeda ◽  
Junling Gao ◽  
Kristopher G. Hooten ◽  
Enyin Wang ◽  
Jason R. Thonhoff ◽  
...  

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Nidhi Saini ◽  
Heinrich Reichert

Neural stem cells inDrosophilaare currently one of the best model systems for understanding stem cell biology during normal development and during abnormal development of stem cell-derived brain tumors. InDrosophilabrain development, the proliferative activity of neural stem cells called neuroblasts gives rise to both the optic lobe and the central brain ganglia, and asymmetric cell divisions are key features of this proliferation. The molecular mechanisms that underlie the asymmetric cell divisions by which these neuroblasts self-renew and generate lineages of differentiating progeny have been studied extensively and involve two major protein complexes, the apical complex which maintains polarity and controls spindle orientation and the basal complex which is comprised of cell fate determinants and their adaptors that are segregated into the differentiating daughter cells during mitosis. Recent molecular genetic work has establishedDrosophilaneuroblasts as a model for neural stem cell-derived tumors in which perturbation of key molecular mechanisms that control neuroblast proliferation and the asymmetric segregation of cell fate determinants lead to brain tumor formation. Identification of novel candidate genes that control neuroblast self-renewal and differentiation as well as functional analysis of these genes in normal and tumorigenic conditions in a tissue-specific manner is now possible through genome-wide transgenic RNAi screens. These cellular and molecular findings inDrosophilaare likely to provide valuable genetic links for analyzing mammalian neural stem cells and tumor biology.


Sign in / Sign up

Export Citation Format

Share Document