scholarly journals Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm

2021 ◽  
Vol 22 (23) ◽  
pp. 12788
Author(s):  
Subin Jeong ◽  
Jisue Kim ◽  
Hye Mi Jeon ◽  
Kyunghee Kim ◽  
Gun Yong Sung
Keyword(s):  
Circadian Rhythm ◽  
Accelerated Aging ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Skin Model ◽  
Flexible Skin ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Aging Skin

The skin is subject to both intrinsic aging caused by metabolic processes in the body and extrinsic aging caused by exposure to environmental factors. Intrinsic aging is an important obstacle to in vitro experimentation as its long-term progression is difficult to replicate. Here, we accelerated aging of a full-thickness skin equivalent by applying periodic mechanical stimulation, replicating the circadian rhythm for 28 days. This aging skin model was developed by culturing a full-thickness, three-dimensional skin equivalent with human fibroblasts and keratinocytes to produce flexible skin-on-a-chip. Accelerated aging associated with periodic compressive stress was evidenced by reductions in the epidermal layer thickness, contraction rate, and secretion of Myb. Increases in β-galactosidase gene expression and secretion of reactive oxygen species and transforming growth factor-β1 were also observed. This in vitro aging skin model is expected to greatly accelerate drug development for skin diseases and cosmetics that cannot be tested on animals.

scholarly journals In vitro 3D Full-Thickness Skin-Equivalent Tissue Model Using Silk and Collagen Biomaterials

2012 ◽  
Vol 12 (12) ◽  
pp. 1627-1636 ◽  
Author(s):  
Evangelia Bellas ◽  
Miri Seiberg ◽  
Jonathan Garlick ◽  
David L. Kaplan
Keyword(s):  
Skin Equivalent ◽  
Full Thickness ◽  
Tissue Model ◽  
Full Thickness Skin ◽  
Thickness Skin

Striae reconstructed, a full thickness skin model that recapitulates the pathology behind stretch marks

2019 ◽  
Vol 41 (3) ◽  
pp. 311-319 ◽  
Author(s):  
M. Perez‐Aso ◽  
A. Roca ◽  
J. Bosch ◽  
B. Martínez‐Teipel
Keyword(s):  
Full Thickness ◽  
Skin Model ◽  
Full Thickness Skin ◽  
Stretch Marks ◽  
Thickness Skin

scholarly journals Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Mahmoud Ameri ◽  
Hayley Lewis ◽  
Paul Lehman
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Full Thickness ◽  
Artificial Membrane ◽  
Skin Model ◽  
Hydrophobic Membrane ◽  
Thickness Skin ◽  
In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

154 Determination of phototoxicity properties of different compounds using a full thickness skin model (AST-2000)

2003 ◽  
Vol 144 ◽  
pp. s44
Author(s):  
J.J. Hoffmann ◽  
E. Heisler ◽  
P. Peters ◽  
S. Karpinski ◽  
H.-W. Vohr
Keyword(s):  
Full Thickness ◽  
Skin Model ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals The Phenion® Full-Thickness Skin Model for Percutaneous Absorption Testing

2010 ◽  
Vol 23 (2) ◽  
pp. 105-112 ◽  
Author(s):  
K. Ackermann ◽  
S. Lombardi Borgia ◽  
H.C. Korting ◽  
K.R. Mewes ◽  
M. Schäfer-Korting
Keyword(s):  
Percutaneous Absorption ◽  
Full Thickness ◽  
Skin Model ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

10.3791/52576 ◽  
2015 ◽  
Author(s):  
Angela Rossi ◽  
Antje Appelt-Menzel ◽  
Szymon Kurdyn ◽  
Heike Walles ◽  
Florian Groeber
Keyword(s):  
Three Dimensional ◽  
Skin Equivalent ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

scholarly journals The Co-Culture of ASCs and EPCs Promotes Wound Healing by Promoting Angiogenesis in Full Thickness Skin Defects

2020 ◽  
Author(s):  
Shuang Lin ◽  
Xiaoning He ◽  
Yuanjia He
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Donor Site ◽  
Skin Tissue ◽  
Skin Defect ◽  
Full Thickness ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats.Result The co-culture of ASCs/EPCs could increase skin and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin.Conclusion It is feasible to replace traditional single seed cells with ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

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