scholarly journals Impact of Metarhizium robertsii on Adults of the Parasitoid Diachasmimorpha longicaudata and Parasitized Anastrepha ludens Larvae

Insects ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 125
Author(s):  
Ehdibaldo Presa-Parra ◽  
Francisco Hernández-Rosas ◽  
Julio S. Bernal ◽  
Jorge E. Valenzuela-González ◽  
Jovita Martínez-Tlapa ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Isaria Fumosorosea ◽  
Negative Effects ◽  
Metarhizium Robertsii ◽  
Low Concentrations ◽  
Combined Use ◽  
Diachasmimorpha Longicaudata

Biological control of the Mexican fruit fly, Anastrepha ludens, is mainly carried out by releasing parasitoids, such as Diachasmimorpha longicaudata, and by applying entomopathogenic fungi (EPF), such as Metarhizium anisopliae, Beauveria bassiana, or Isaria fumosorosea, which can be applied to the soil or dispersed using infective devices. The combined use of two or more biocontrol agents could improve A. ludens control, but IGP between natural enemies, if it occurs, may have negative effects. We evaluated the effects of EPF on D. longicaudata. First, we determined the susceptibility of adults of D. longicaudata to strains of EPF (Metarhizium robertsii strain V3-160 and M. anisopliae strain MAAP1). We also evaluated the infection of these two fungi on A. ludens larvae parasitized by D. longicaudata. Finally, we determined sub-lethal effects on adults of D. longicaudata that emerged from larvae that had been exposed to low concentrations of M. robertsii. Both fungi caused moderate mortality to D. longicaudata adults. There were no adverse effects on the longevity of parasitoids that emerged from parasitized larvae exposed to M. robertsii. Based on these results, we argue that M. robertsii has the potential to be used for biocontrol of A. ludens, with limited risk to D. longicaudata adults.

Occurrence, Identification, and Virulence of Native Fungal Pathogens Isolated From Mexican Fruit Fly (Diptera: Tephritidae) Larvae From Soils of Three Cropping Systems

2020 ◽  
Vol 113 (3) ◽  
pp. 1088-1096
Author(s):  
Ehdibaldo Presa-Parra ◽  
Francisco Hernández-Rosas ◽  
Julio S Bernal ◽  
Jorge E Valenzuela-González ◽  
Alma Altúzar-Molina ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Agricultural Soils ◽  
Biological Control Agent ◽  
Cropping Systems ◽  
Elongation Factor ◽  
Fruit Fly ◽  
Control Agent ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Metarhizium Robertsii

Abstract The Mexican fruit fly, Anastrepha ludens Loew, is a significant pest in mango and citrus production areas of Mexico. In this study, we evaluated the effects of some geographic characteristics, rainfall period, soil micro-environmental, and soil coverage variables on the occurrence of entomopathogenic fungi (EPF) associated with A. ludens larvae in soils of mango, grapefruit and mixed crops in central Veracruz state, Mexico. EPF isolates were characterized morphologically and identified by sequence analysis of elongation factor (EF1-1018F, EF1-1620R). We recorded four species of EPF (Metarhizium robertsii J.F. Bisch, S.A. Rehner & Humber [Hypocreales: Cordycipitaceae], M. brunneum Petch [Hypocreales: Cordycipitaceae], M. pinghaense Q.T. Chen & H.L. Guo [Hypocreales: Cordycipitaceae], and Beauveria bassiana (Balsamo) Vuillemin [Hypocreales: Cordycipitaceae]), of which Metarhizium robertsii was the most abundant and the most virulent. Also, we found that rainfall period, organic matter, coverage of herbs and forbs, and calcium levels modulated EPF occurrence. We estimated lethal concentrations for A. ludens larvae of the four most promising isolates, V3-123, V3-160, V1-332, and V3-369. Our results suggest that M. robertsii obtained from agricultural soils holds potential as a biological control agent for A. ludens.

Effect of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) on food consumption and mortality in the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae)

2018 ◽  
Vol 38 (03) ◽  
pp. 254-260 ◽  
Author(s):  
Ricardo Alberto Toledo-Hernández ◽  
Jorge Toledo ◽  
Daniel Sánchez
Keyword(s):  
Pest Management ◽  
Food Consumption ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Sublethal Effects ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Important Pest ◽  
Highly Virulent

AbstractAnastrepha ludens (Loew) (Diptera: Tephritidae) is an important pest of mango and citrus in Mexico. Here, we report the effect of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin strain MAAP1 on mortality and food consumption in A. ludens adults. Experiments were carried out under laboratory conditions at 26 ± 2 °C, 60 ± 5% RH and 12:12 photoperiod. To assess pathogenicity and sublethal effects, we sprayed flies with 1 × 109 conidia/ml and 1 × 106 conidia/ml solutions, respectively. Food consumption was measured by providing known amounts of food through capillary tubes to caged flies. We found that M. anisopliae was highly virulent to A. ludens, causing 92.3 ± 0.62% mortality at day 13 post-exposure. Interestingly, food consumption reduced by 17% in flies exposed to the fungus. These results support the implementation of M. anisopliae within current strategies used for the integrated pest management of A. ludens.

scholarly journals Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae)

EDIS ◽  
1969 ◽  
Vol 2004 (5) ◽  
Author(s):  
Howard V. Weems, Jr. ◽  
John B. Heppner ◽  
Thomas R. Fasulo ◽  
James L. Nation
Keyword(s):  
Fruit Fly ◽  
Cooperative Extension Service ◽  
Anastrepha Ludens ◽  
Anastrepha Suspensa ◽  
Mexican Fruit Fly ◽  
The West ◽  
University Of Florida ◽  
Caribbean Fruit Fly ◽  
Guava Fruit ◽  
The Caribbean

The Caribbean fruit fly, Anastrepha suspensa (Loew), has also been called the Greater Antilliean fruit fly, the guava fruit fly and the Caribfly. It is a near relative of the Mexican fruit fly, Anastrepha ludens (Loew), and is one of several species of fruit flies which are indigenous to the West Indies and the larvae of which attack several kinds of tropical and subtropical fruits. This document is EENY-196 (originally published as DPI Entomology Circulars 38 and 260), one of a series of Featured Creatures from the Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published: March 2001. EENY196/IN353: Caribbean Fruit Fly, Anastrepha suspensa (Loew) (Insecta: Diptera: Tephritidae) (ufl.edu)

scholarly journals Host Marking Pheromone (HMP) in the Mexican Fruit Fly Anastrepha ludens

2010 ◽  
Vol 64 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Andrew J. F. Edmunds ◽  
Martin Aluja ◽  
Fransico Diaz-Fleischer ◽  
Bruno Patrian ◽  
Leonhard Hagmann
Keyword(s):  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Marking Pheromone ◽  
Host Marking Pheromone

Superparasitism strategies by a native and an exotic parasitoid species attacking the Mexican fruit fly,Anastrepha ludens(Diptera: Tephritidae)

2014 ◽  
Vol 24 (8) ◽  
pp. 925-935 ◽  
Author(s):  
Amanda Ayala ◽  
Ana Mabel Martinez ◽  
Isaac Figueroa ◽  
Samuel Pineda ◽  
Mario Miranda ◽  
...  
Keyword(s):  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Parasitoid Species

Comparisons of Cobalt-60 Gamma Irradiation Dose Rates on Grapefruit Flavedo Tissue and on Mexican Fruit Fly Mortality

1990 ◽  
Vol 53 (4) ◽  
pp. 329-331 ◽  
Author(s):  
GENE E. LESTER ◽  
DAN A. WOLFENBARGER
Keyword(s):  
Gamma Irradiation ◽  
Dose Rate ◽  
Electrolyte Leakage ◽  
Irradiation Dose ◽  
Adult Emergence ◽  
Fruit Fly ◽  
Anastrepha Ludens ◽  
Mexican Fruit Fly ◽  
Dose Rates ◽  
Grapefruit Peel

Percent electrolyte leakage, a measure of membrane integrity, proved to be a good predictor (R2 = 0.99) of cobalt-60 gamma irradiation dose injury on mid-season ‘Ruby Red’ grapefruit Citrus paradisi (Macf.) flavedo tissue (peel). Percent electrolyte leakage on grapefruit peel following a dose-rate of 250 grays/1.0, 2.5, 5.0, and 25.0 min decreased as grays/min (dose-rate) decreased. Total phenols, a biochemical response to irradiation following 250 grays/1.0, 2.5, 5.0, and 25.0 min also decreased as dose-rate decreased, demonstrating that injury to grapefruit peel diminished as 250 grays of gamma irradiation/rate declined. Comparisons of 10 and 20 grays of cobalt-60 gamma irradiation showed that a dose-rate of 10 grays/0.25 min to naked 8-d old Mexican fruit fly Anastrepha ludens (Loew) larvae caused a 90% reduction of adult emergence. Whereas, at 20 grays the reduction was greater than 99% with dose-rates of 20 grays/0.25, 0.5, 1.0, or 100 min. These data show that a gamma irradiation dose capable of reducing fly emergence by >99% will maintain an inhibitory effect even at relatively lower dose-rates. Therefore, once a quarantine security treatment for Mexican fruit fly is established, a lower dose-rate will reduce adult emergence and should impart little damage to grapefruit peel tissue.

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