An Integrative Study on Asphondylia spp. (Diptera: Cecidomyiidae), Causing Flower Galls on Lamiaceae, with Description, Phenology, and Associated Fungi of Two New Species

An integrative study on some species of Asphondylia was carried out. Two species of gall midges from Italy, Asphondylia rivelloi sp. nov. and Asphondylia micromeriae sp. nov. (Diptera: Cecidomyiidae), causing flower galls respectively on Clinopodium vulgare and Micromeria graeca (Lamiaceae), are described and illustrated. The characteristics of each developmental stage and induced galls are described, which allowed the discrimination of these new species in the complex of Asphondylia developing on Lamiaceae plants. Molecular data based on sequencing both nuclear (ITS2 and 28S-D2) and mitochondrial (COI) genes are also provided in support of this discrimination. Phylogeny based on nuclear markers is consistent with the new species, whereas COI phylogeny suggests introgression occurring between the two species. However, these species can also be easily identified using a morphological approach. Phenology of host plants and gall midges are described, and some peculiar characteristics allow the complete and confident discrimination and revision of the treated species. Gall-associated fungi were identified as Botryosphaeria dothidea,Alternaria spp., and Cladosporium spp.

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Description of two new species of Rhamphus related to R. oxyacanthae (Curculionidae, Curculioninae, Rhamphini) from Italy based on a morphological study supported by molecular data

Zootaxa ◽

10.11646/zootaxa.4995.1.6 ◽

2021 ◽

Vol 4995 (1) ◽

pp. 111-128

Author(s):

LUCIANO DIOTTI ◽

ROBERTO CALDARA ◽

IVO TOŠEVSKI

Keyword(s):

New Species ◽

Morphological Study ◽

Host Plants ◽

Molecular Study ◽

Morphological Characters ◽

Molecular Data ◽

Integrative Taxonomy ◽

The Other ◽

Distribution Data ◽

Two New Species

Two new species of the weevil genus Rhamphus from Italy are herein described: R. bavierai n. sp. (Sicily) and R. hampsicora n. sp. (Sardinia). Both are morphologically and from a molecular perspective close to R. oxyacanthae (Marsham, 1802) and R. monzinii Pesarini & Diotti, 2012. Aside from a diagnostic description and a synoptic key, distribution data and notes on the host plants of the four species are reported. Whereas R. monzinii can be distinguished by several morphological characters, the other three species are morphologically very similar to each other and separable only by the combination of a few subtle characters. On the contrary, a preliminary molecular study revealed substantial divergences of mtCOI from 6.2 to 14.9% between the species, confirming the importance of an integrative taxonomy.

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Two new species of Atlantoscia Ferrara & Taiti, 1981 (Isopoda: Oniscidea: Philosciidae) from southern Brazil described in the light of integrative taxonomy

Zootaxa ◽

10.11646/zootaxa.4482.3.7 ◽

2018 ◽

Vol 4482 (3) ◽

pp. 551 ◽

Cited By ~ 3

Author(s):

BIANCA LAÍS ZIMMERMANN ◽

IVANKLIN SOARES CAMPOS-FILHO ◽

GIOVANNA MONTICELLI CARDOSO ◽

SANDRO SANTOS ◽

JOSÉ OTÁVIO AGUIAR ◽

...

Keyword(s):

New Species ◽

Comparative Morphology ◽

Molecular Data ◽

Integrative Taxonomy ◽

Southern Brazil ◽

Terrestrial Isopods ◽

Species Discovery ◽

Mitochondrial Coi ◽

Taxonomic Impediment ◽

Two New Species

The use of molecular data in association with classical taxonomy has helped to alleviate the taxonomic impediment through the discovery, delimitation and description of new species. Terrestrial isopods are the largest suborder of Isopoda; however, there are very few active taxonomists in this group and a representative part of the terrestrial isopods world diversity remains unexplored. The genus Atlantoscia comprises five species in which diagnostic characters are few and show subtle differences among the species. The objective of this study was to delimit and describe two new species of the genus Atlantoscia from southern Brazil by using traditional taxonomy coupled with genetic information. Specimens were analyzed morphologically and by the aid of two molecular markers, mitochondrial COI and nuclear 18S rRNA. The validity of the new species Atlantoscia antennamaculata sp. nov. and Atlantoscia australis sp. nov. was corroborated, unambiguously, by morphological approach, phylogenetic analysis and species delimitation methods. Our study reinforces the fact that, despite the undeniable importance of comparative morphology in species discovery, new methods and data, particularly molecular ones, are becoming prominent and an integrative part of the taxonomy practice.

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Two new species of Litostigma (Gesneriaceae) from the China–Vietnam border area based on morphological and molecular data, adding new stigma characters for the genus

Nordic Journal of Botany ◽

10.1111/njb.02971 ◽

2021 ◽

Vol 39 (4) ◽

Author(s):

Chen You‐Sheng ◽

Yun‐Feng Huang ◽

Lian‐Sheng Xu ◽

Bing‐Mou Wang

Keyword(s):

New Species ◽

Molecular Data ◽

Border Area ◽

Two New Species ◽

Morphological And Molecular Data

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Xylodon bambusinus and X. xinpingensis spp. nov. (Hymenochaetales) from southern China

Phytotaxa ◽

10.11646/phytotaxa.511.3.3 ◽

2021 ◽

Vol 511 (3) ◽

Author(s):

XIANG MA ◽

CHANG-LIN ZHAO

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Growth Habit ◽

Southern China ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Two New Species ◽

Thin Walled ◽

Inference Methods

Two new species, Xylodon bambusinus and X. xinpingensis, are proposed based on morphological and molecular evidences. Both species share the annual growth habit, resupinate basidiomata and monomitic hyphal system with clamped, colorless generative hyphae, smooth, thin-walled basidiospores, but X. bambusinus is characterized by the smooth to tuberculate hymenial surface, presence of capitate and fusiform cystidia, broad ellipsoid basidiospores, while X. xinpingensis by the reticulate hymenophore with cream hymenial surface, and subglobose basidiospores (4.5–6 × 3.5–5 µm). Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on molecular data of ITS and ITS+nLSU sequences showed that X. bambusinus was sister to X. subclavatus, while X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus. The nLSU dataset revealed that X. bambusinus grouped with X. asperus and X. brevisetus with lower supports, and that X. xinpingensis grouped with X. astrocystidiatus and X. paradoxus and then with X. rimosissimus without supports. Both morphological and molecular evidences confirmed the placement of two new species in Xylodon. Description and figures from the new species and a key to the known species of Xylodon from China are presented.

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Five new deep-sea species of nudibranchs (Gastropoda: Heterobranchia: Cladobranchia) from the Northeast Pacific

Zootaxa ◽

10.11646/zootaxa.4526.4.1 ◽

2018 ◽

Vol 4526 (4) ◽

pp. 401 ◽

Cited By ~ 3

Author(s):

ÁNGEL VALDÉS ◽

LONNY LUNDSTEN ◽

NERIDA G. WILSON

Keyword(s):

Climate Change ◽

New Species ◽

Deep Sea ◽

Undescribed Species ◽

Invertebrate Community ◽

Nuclear Markers ◽

Mitochondrial Coi ◽

Northeast Pacific ◽

Northeastern Pacific ◽

System Information

Increased exploration of northeastern Pacific deep-sea habitats has revealed a diverse and often poorly-known invertebrate community, including a number of undescribed species of nudibranchs studied herein. We used morphology to distinguish several new species from their congeners, and generated data where possible for mitochondrial (COI, 16S) and nuclear markers (H3) to place them in a phylogenetic context. We described here Tritonia nigritigris sp. nov., Dendronotus claguei sp. nov., Ziminella vrijenhoeki sp. nov., Cuthona methana sp. nov., Aeolidia libitinaria sp. nov. and redescribed Zeusia herculea (Bergh, 1894). Another species of Tritonia is described but not named due to the absence of reproductive system information. Although there are difficulties in collection from deep-sea habitats, only two of our new species are known from single specimens. As with many other deep-sea regions, we expect the number of new species from this region to increase with further exploration. Because the deep regions of the northeast Pacific are particularly vulnerable to the effects of decreasing oxygenation due to climate change, we consider that documenting this fauna has some level of urgency.

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New gall midges (Diptera, Cecidomyiidae) associated with Eugenia uniflora and Psidium cattleianum (Myrtaceae)

Iheringia Série Zoologia ◽

10.1590/s0073-47212011000100010 ◽

2011 ◽

Vol 101 (1-2) ◽

pp. 69-74 ◽

Cited By ~ 1

Author(s):

Valéria C Maia ◽

Dori E Nava

Keyword(s):

New Species ◽

New Genus ◽

Gall Midges ◽

Eugenia Uniflora ◽

Psidium Cattleianum ◽

Two New Species

Two new species and a new genus of gall midges (Diptera, Cecidomyiidae) are described and illustrated. Both species induce leaf galls on Myrtaceae, the former on Eugenia uniflora and the latter on Psidium cattleianum.

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Two new species of Bimastos (Oligochaeta, Lumbricidae) from the Southern Appalachian Mountains, North America

Zootaxa ◽

10.11646/zootaxa.5052.3.5 ◽

2021 ◽

Vol 5052 (3) ◽

pp. 395-405

Author(s):

ROBERTO CARRERA-MARTÍNEZ ◽

DANIEL JONES ◽

SEAN D. SCHOVILLE ◽

BRUCE A. SNYDER ◽

MAC A. JR. CALLAHAM

Keyword(s):

New Species ◽

Body Size ◽

North America ◽

Appalachian Mountains ◽

Molecular Data ◽

Southern Appalachian Mountains ◽

Southern Appalachian ◽

Two New Species ◽

Morphological And Molecular Data ◽

Posterior Position

Two new species of Bimastos Moore are described based on morphological and molecular data. Bimastos nanae n. sp. resembles B. lawrenceae Fender, B. zeteki (Smith and Gittins) and B. welchi (Smith). Bimastos nanae n. sp. differs from these species in the position of the clitellum, size and number and position of thickened septa. Bimastos magnum n. sp. is similar to B. schwerti Csuzdi & Chang and B. palustris Moore in having a fully annular clitellum and male pores on huge porophores. Bimastos magnum n. sp. differs from both species by having a more posterior position of the clitellum (in xxiv-xxxiii, xxxiv) and larger body size. With the description of these new species, the number of Bimastos species is raised to 14.

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New Genus and Species of Gall Midges (Diptera, Cecidomyiidae, Porricondylinae, Holoneurini) from the Late Eocene Amber of Olevsk (Zhitomir Region, Ukraine)

Vestnik Zoologii ◽

10.1515/vzoo-2017-0004 ◽

2017 ◽

Vol 51 (1) ◽

pp. 23-30 ◽

Cited By ~ 5

Author(s):

Z. A. Fedotova ◽

E. E. Perkovsky

Keyword(s):

New Species ◽

New Genus ◽

Late Eocene ◽

Rovno Amber ◽

New Genus And Species ◽

Gall Midges ◽

Two New Species ◽

First Time ◽

Eocene Amber

Abstract Gall midges are reported for the first time in Late Eocene Rovno amber from the Olevsk, Zhitomir Region. This is the second amber locality to yield gall midges in the Zhitomir Region, after Gulyanka. Rovnoholoneurus gen. n. and two new species, Rovnoholoneurus davidi sp. n. and R. miyae sp. n. are described. Bryocrypta laqueata Fedotova, 2005 is transferred to the genus Rovnoholoneurus, and Rovnoholoneurus laqueatus (Fedotova, 2005), comb. n. is established. A key to the species of Rovnoholoneurus is provided.

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The genus Parasola in Pakistan with the description of two new species

MycoKeys ◽

10.3897/mycokeys.30.21430 ◽

2018 ◽

Vol 30 ◽

pp. 41-60 ◽

Cited By ~ 2

Author(s):

Shah Hussain ◽

Habib Ahma ◽

Sadiq Ullah ◽

Najam-Ul-Sehar Afshan ◽

Donald H. Pfister ◽

...

Keyword(s):

New Species ◽

Elongation Factor ◽

Molecular Data ◽

Internal Transcribed Spacers ◽

Side View ◽

Germ Pore ◽

5.8S Rdna ◽

Rdna Its ◽

Two New Species ◽

Morphological And Molecular Data

Parasola is a genus of small, veil-less coprinoid mushrooms in the family Psathyrellaceae (Agaricales). The genus is not well documented in Asia, specifically in Pakistan. In this study we describe two new species Parasolaglabra and P.pseudolactea from Pakistan, based on morphological and molecular data. Phylogeny based on three DNA regions: nuc rDNA region encompassing the internal transcribed spacers 1 and 2 along with the 5.8S rDNA (ITS), nuc 28S rDNA D1-D2 domains (28S) and translation elongation factor 1α gene (TEF1α) show that the new taxa are clustered in a clade formed by the members of section Parasola of genus Parasola. Parasolaglabra with grayish pileus, slightly depressed pileal disc, lamellae separated from the stipe by pseudocollarium, basidiospores 14.5–16.5 × 9.5–11.5 × 8.0–10.5 µm, in front view broadly ovoid to oblong, some with rhomboidal outline, in side view ellipsoid, with eccentric germ-pore of 1.5 µm diameter. Parasolapseudolactea with yellowish brown to dull brown pileus, disc indistinctly umbonate, lamellae free, pseudocollarium absent, basidiospores 13.5–14.5 × 10.5–12.0 × 9.5–10.5 µm, in face view rounded triangular to heart shaped, rarely ovoid to subglobose, in side view ellipsoid to oblong, with eccentric germ-pore of 1.5 µm diam. In addition to these new species, P.auricoma and P.lilatincta were also studied. Morphological descriptions for the new species and comparison with known Parasola species are provided. Our observations highlight the diversity of Parasola in northern Pakistan and further document the need for additional systematic focus on the region’s fungi.

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