scholarly journals Clinical Outcome and 8-Year Follow-Up of Alveolar Bone Tissue Engineering for Severely Atrophic Alveolar Bone Using Autologous Bone Marrow Stromal Cells with Platelet-Rich Plasma and β-Tricalcium Phosphate Granules

2021 ◽  
Vol 10 (22) ◽  
pp. 5231
Author(s):  
Izumi Asahina ◽  
Hideaki Kagami ◽  
Hideki Agata ◽  
Masaki J. Honda ◽  
Yoshinori Sumita ◽  
...  

Background: Although bone tissue engineering for dentistry has been studied for many years, the clinical outcome for severe cases has not been established. Furthermore, there are limited numbers of studies that include long-term follow-up. In this study, the safety and efficacy of bone tissue engineering for patients with a severely atrophic alveolar bone were examined using autogenous bone marrow stromal cells (BMSCs), and the long-term stability was also evaluated. Methods: BMSCs from iliac bone marrow aspirate were cultured and expanded. Then, induced osteogenic cells were transplanted with autogenous platelet-rich plasma (PRP) and β-tricalcium phosphate granules (β-TCP) for maxillary sinus floor and alveolar ridge augmentation. Eight patients (two males and six females) with an average age of 54.2 years underwent cell transplantation. Safety was assessed by monitoring adverse events. Radiographic evaluation and bone biopsies were performed to evaluate the regenerated bone. Results: The major population of transplanted BMSCs belonged to the fraction of CD34−, CD45dim, and CD73+ cells, which was only 0.065% of the total bone marrow cells. Significant deviations were observed in cell growth and alkaline phosphatase activities among individuals. However, bone regeneration was observed in all patients and the average bone area in the biopsy samples was 41.9% 6 months following transplantation, although there were also significant deviations among each case. No adverse events related to the transplants were observed. In the regenerated bone, 27 out of 29 dental implants were integrated. Dental implants and regenerated bone were stable for an average follow-up period of 7 years and 10 months. Conclusions: Although individual variations were observed, the results showed that bone tissue engineering using BMSCs with PRP and β-TCP was feasible for patients with severe atrophic maxilla throughout a long-term follow-up period and was considered safe. However, further studies with a larger number of cases and controls to confirm the efficacy of BMSCs and the development of a protocol to establish a reproducible quality of stem cell-based graft material will be required.

2011 ◽  
Vol 26 (8) ◽  
pp. 1035-1049 ◽  
Author(s):  
Federico Foschi ◽  
Enrico Conserva ◽  
Paolo Pera ◽  
Barbara Canciani ◽  
Ranieri Cancedda ◽  
...  

2008 ◽  
Vol 368-372 ◽  
pp. 1235-1237 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Wei Dong Tian ◽  
Sheng Wei Li

Hydroxyapatite-tricalcium phosphate (HA-TCP) is a new kind of material which shows good biocompatibility, biological degradability, and porosity. This study aimed to determine the effectiveness of HA-TCP as a bone tissue engineering scaffold. In this study, critical size cranial defects were reconstructed with compounds of autogenous bone marrow stromal cells (BMSCs) and HA-TCP. The resulting grafts were examined by X-ray, histological examination, semi-quantitative analysis of osteogenesis, immunochemical examination (collagen type I and III), scanning electron microscopy and transmission electron microscopy. The results showed that HA-TCP is a good bone tissue engineering scaffold and BMSCs/HA-TCP is a promising technique for reconstruction of bone defects.


2002 ◽  
Vol 8 (6) ◽  
pp. 941-953 ◽  
Author(s):  
Karine Anselme ◽  
Odile Broux ◽  
Benoit Noel ◽  
Bertrand Bouxin ◽  
Gerard Bascoulergue ◽  
...  

2015 ◽  
Vol 120 (5) ◽  
pp. 570-576 ◽  
Author(s):  
Hirokazu Akiyama ◽  
Asako Kobayashi ◽  
Masaki Ichimura ◽  
Hiroshi Tone ◽  
Masaru Nakatani ◽  
...  

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