scholarly journals In Vivo Confocal Microscopy of the Corneal-Conjunctival Transition in the Evaluation of Epithelial Renewal after SLET

2020 ◽  
Vol 9 (11) ◽  
pp. 3574
Author(s):  
Emilio Pedrotti ◽  
Chiara Chierego ◽  
Tiziano Cozzini ◽  
Tommaso Merz ◽  
Neil Lagali ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Case Series ◽  
In Vivo Confocal Microscopy ◽  
Corneal Surface ◽  
Visual Rehabilitation ◽  
Central Cornea ◽  
Epithelial Phenotype ◽  
The Stability ◽  
Cell Phenotypes

Examination of the corneal surface by in vivo confocal microscopy (IVCM) allows for objective identification of corneal and conjunctival cell phenotypes to evaluate different epithelialization patterns. Detection of a corneal-conjunctival epithelial transition could be considered as a sign of restored epithelial function following simple limbal epithelial transplantation (SLET). This is a prospective, interventional case series. We assessed patients with limbal stem cell deficiency (LSCD) by IVCM, preoperatively and at monthly intervals following SLET. Sectors in the central and peripheral cornea were scanned. Immediately upon detection of multi-layered cells with the epithelial phenotype in the central cornea and confirmation of epithelial transition in all corneal sectors, the decision for keratoplasty was taken. Ten patients were enrolled. After SLET, epithelial phenotype in the central cornea and an epithelial transition were identified within six and nine months in seven and one patients, respectively. One patient was a partial success and one failed. Five patients underwent keratoplasty, with stable results up to 12 months. Identification of the epithelial transition zone by IVCM permits assessment of the efficacy of SLET, enabling subsequent planning of keratoplasty for visual rehabilitation. The stability of the corneal surface following keratoplasty confirms that the renewal of the corneal epithelium was effectively retained.

Multimodal imaging of pre-Descemet corneal dystrophy

2019 ◽  
Vol 30 (5) ◽  
pp. 908-916 ◽  
Author(s):  
Munirah Alafaleq ◽  
Cristina Georgeon ◽  
Kate Grieve ◽  
Vincent M Borderie
Keyword(s):  
Optical Coherence Tomography ◽  
Confocal Microscopy ◽  
Multimodal Imaging ◽  
Case Series ◽  
Corneal Dystrophy ◽  
Spectral Domain ◽  
Optical Coherence ◽  
In Vivo Confocal Microscopy ◽  
Descemet Membrane

Purpose: The aim of this study was to assess structural and histological changes associated with pre-Descemet corneal dystrophy with multimodal in vivo imaging. Methods: Retrospective case series including eight corneas from four unrelated male patients with pre-Descemet corneal dystrophy characterized by the presence of punctiform gray opacities located just anterior to the Descemet membrane at slit-lamp examination of both eyes. In vivo confocal microscopy images were obtained in the central, paracentral, and peripheral corneal zones from the superficial epithelial cell layer down to the corneal endothelium in both eyes. Spectral domain optical coherence tomography scans (central and limbal zones) and mapping of both corneas were acquired. Results: Diffuse small extracellular stromal deposits, presence of enlarged hyperreflective keratocytes in the posterior stroma with either hyperreflective or hyporeflective intracellular dots, and presence of activated keratocytes in the very anterior stroma were observed in all corneas with in vivo confocal microscopy. Spectral domain optical coherence tomography scans showed a hyperreflective line anterior to Descemet’s membrane running from limbus to limbus and associated with a second thinner hyperreflective line just beneath Bowman’s layer. Fine hyperreflective particles were observed in the posterior, mid, and anterior stroma on optical coherence tomography scans. Conclusion: The clinical presentation and structural anomalies found in isolated sporadic pre-Descemet corneal dystrophy are in favor of a degenerative process affecting corneal keratocytes with no epithelial or endothelial involvement. The maximum damage is found just anterior to the Descemet membrane resulting in pre-Descemet membrane location of stromal opacities. Multimodal imaging of cornea reveals that the disorder affects the whole stroma and it permits better understanding of pre-Descemet corneal dystrophy pathophysiology together with ascertained diagnosis.

Microstructural evaluation of the mucin balls and their relations to the corneal surface—Insights by in vivo confocal microscopy

2017 ◽  
Vol 40 (5) ◽  
pp. 340-345 ◽  
Author(s):  
Christina N. Grupcheva ◽  
Dimitar I. Grupchev ◽  
Mladena N. Radeva ◽  
Desislava I. Vankova ◽  
Yana M. Manolova
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy ◽  
Corneal Surface ◽  
Microstructural Evaluation

Corneal Nerve and Epithelial Cell Alterations in Corneal Allodynia: An In Vivo Confocal Microscopy Case Series

2017 ◽  
Vol 15 (1) ◽  
pp. 139-151 ◽  
Author(s):  
Pedram Hamrah ◽  
Yureeda Qazi ◽  
Bashar Shahatit ◽  
Mohammad H. Dastjerdi ◽  
Deborah Pavan-Langston ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Confocal Microscopy ◽  
Case Series ◽  
In Vivo Confocal Microscopy ◽  
Corneal Nerve ◽  
Cell Alterations

Dendritiform Cells Found in Central Cornea by In-Vivo Confocal Microscopy in a Patient with Mixed Bacterial Keratitis

2006 ◽  
Vol 14 (4) ◽  
pp. 241-244 ◽  
Author(s):  
Pei-Yuang Su ◽  
Fung-Rong Hu ◽  
Yen-Ming Chen ◽  
Jen-Hui Han ◽  
Wei-Li Chen
Keyword(s):  
Confocal Microscopy ◽  
Bacterial Keratitis ◽  
In Vivo Confocal Microscopy ◽  
Central Cornea

scholarly journals Structural Changes in Thin Keratoconic Corneas Following Crosslinking with Hypotonic Riboflavin: Findings on In Vivo Confocal Microscopy

2021 ◽  
Author(s):  
Aalia Rasool Sufi ◽  
M. Soundaram ◽  
Nilam Gohil ◽  
Jeremy D. Keenan ◽  
N. Venkatesh Prajna
Keyword(s):  
Confocal Microscopy ◽  
Structural Changes ◽  
Corneal Thickness ◽  
Clinical Signs ◽  
Case Series ◽  
Controlled Study ◽  
In Vivo Confocal Microscopy ◽  
Collagen Crosslinking ◽  
Endothelial Cell Count

Purpose: To report structural changes observable in in vivo confocal microscopy (IVCM) in keratoconic corneas <400 μm treated with hypotonic riboflavin and collagen crosslinking (CXL). Method: Ten eyes of ten patients with progressive keratoconus and corneal thickness between 350 and 399 μm underwent CXL with hypotonic riboflavin. IVCM was performed preoperatively and at one month, three months, and six months after the procedure. Results: IVCM analysis one month postoperatively showed complete absence of the subepithelial nerve plexus with gradual regeneration over six months in 8 of the 10 eyes, and poor regeneration in the remaining 2 eyes. The anterior stroma showed extracellular lacunae and hyper-reflective cytoplasm in a honeycomb appearance signifying edema at one month which gradually decreased over six months post CXL. Stromal keratocyte apoptosis was evident in the anterior stroma in all cases and extended to the posterior stroma in four eyes with gradual regeneration evident at three and six months. The specular endothelial count decreased by 8% (P = 0.005) post-CXL, but no corneas developed clinical signs of endothelial trauma. Conclusion: IVCM analysis of thin corneas after hypotonic CXL showed posterior corneal structural changes. Posterior stromal changes were accompanied by a decrease in the endothelial cell count. This case series was a preliminary feasibility study that might necessitate conducting a well-designed controlled study.

In vivo confocal microscopy of a family with schnyder crystalline corneal dystrophy11The authors have no proprietary interest in the equipment used in this study.

Ophthalmology ◽  
1999 ◽  
Vol 106 (5) ◽  
pp. 944-951 ◽  
Author(s):  
Minna H Vesaluoma ◽  
Tuuli U Linna ◽  
Eeva-Marja Sankila ◽  
Jayne S Weiss ◽  
Timo M.T Tervo
Keyword(s):  
Confocal Microscopy ◽  
In Vivo Confocal Microscopy
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