scholarly journals Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with Paracoccidioides brasiliensis: More Is Not Necessarily Better

2020 ◽  
Vol 6 (4) ◽  
pp. 311
Author(s):  
Calliandra M. de-Souza-Silva ◽  
Fabián Andrés Hurtado ◽  
Aldo Henrique Tavares ◽  
Getúlio P. de Oliveira ◽  
Taina Raiol ◽  
...  

Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to P. brasiliensis to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with P. brasiliensis, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model.

1980 ◽  
Vol 28 (2) ◽  
pp. 381-386
Author(s):  
C Sadarangani ◽  
E Skamene ◽  
P A Kongshavn

The characteristics of the mononuclear phagocytes mediating resistance to infection with Listeria during the early phase (0 to 48 h) of the response have been investigated in genetically determined susceptible (A/J) and resistant (C57BL/6, B10.A/SgSn) strains of mice. Irradiation immediately before infection profoundly enhanced the bacterial growth in the resistant strain, while having no effect in the susceptible strain, over a wide range (3 x 10(3) to 10(5)) of infective doses. This effect of irradiation is demonstrable at low-dose radiation (200 roentgens) and can be reversed by repopulation with 20 x 10(6) syngeneic nucleated bone marrow cells. Administration of dextran sulfate 500 24 h before infection profoundly enhanced the bacterial growth in the susceptible strain, while having much less effect in the resistant strain. Thus, the genetic advantage of the resistant mouse strains to listerial infection, at least during the early phase of the response, appears to be due to a cellular mechanism that is highly radiosensitive and relatively insensitive to dextran sulfate 500. In the susceptible strain, the early protective cellular mechanism is radioresistant and highly dextran sulfate 500 sensitive.


1999 ◽  
Vol 96 (26) ◽  
pp. 15056-15061 ◽  
Author(s):  
L. Santambrogio ◽  
A. K. Sato ◽  
G. J. Carven ◽  
S. L. Belyanskaya ◽  
J. L. Strominger ◽  
...  

2005 ◽  
Vol 35 (11) ◽  
pp. 3364-3375 ◽  
Author(s):  
Liang Ma ◽  
Kwok-Wah Chan ◽  
Nigel J. Trendell-Smith ◽  
Adrian Wu ◽  
Lina Tian ◽  
...  

PLoS ONE ◽  
2007 ◽  
Vol 2 (9) ◽  
pp. e957 ◽  
Author(s):  
Marielle C. Gold ◽  
Tammie L. Robinson ◽  
Matthew S. Cook ◽  
Laura K. Byrd ◽  
Heather D. Ehlinger ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document