scholarly journals Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy

2020 ◽  
Vol 10 (4) ◽  
pp. 229
Author(s):  
Anna Wojakowska ◽  
Aneta Zebrowska ◽  
Agata Skowronek ◽  
Tomasz Rutkowski ◽  
Krzysztof Polanski ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Energy Metabolism ◽  
Pilot Study ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Gas Chromatography Mass Spectrometry ◽  
Serum Samples ◽  
Before And After ◽  
And Control

Background: In general, the serum metabolome reflects the patient’s body response to both disease state and implemented treatment. Though serum-derived exosomes are an emerging type of liquid biopsy, the metabolite content of these vesicles remains under researched. The aim of this pilot study was to compare the metabolite profiles of the whole serum and serum-derived exosomes in the context of differences between cancer patients and healthy controls as well as patients’ response to radiotherapy (RT). Methods: Serum samples were collected from 10 healthy volunteers and 10 patients with head and neck cancer before and after RT. Metabolites extracted from serum and exosomes were analyzed by the gas chromatography–mass spectrometry (GC–MS). Results: An untargeted GC–MS-based approach identified 182 and 46 metabolites in serum and exosomes, respectively. Metabolites that differentiated cancer and control samples, either serum or exosomes, were associated with energy metabolism. Serum metabolites affected by RT were associated with the metabolism of amino acids, sugars, lipids, and nucleotides. Conclusions: cancer-related features of energy metabolism could be detected in both types of specimens. On the other hand, in contrast to RT-induced changes observed in serum metabolome, this pilot study did not reveal a specific radiation-related pattern of exosome metabolites.

scholarly journals Metabolic profiles of whole serum and serum-derived exosomes are different in head and neck cancer patients treated by radiotherapy

2020 ◽  
Author(s):  
Anna Wojakowska ◽  
Aneta Zebrowska ◽  
Agata Skowronek ◽  
Tomasz Rutkowski ◽  
Krzysztof Polański ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Energy Metabolism ◽  
Pilot Study ◽  
Head And Neck ◽  
Cancer Patients ◽  
Serum Levels ◽  
The Other ◽  
Metabolite Profiles ◽  
And Control ◽  
Control Samples

Abstract Background Serum metabolome reflects a general patient's body response to both disease state (e.g., cancer) and implemented treatment (e.g., radiotherapy, RT). Though serum-derived exosomes are an emerging type of liquid biopsy, a metabolite content of these vesicles remains under-researched yet. In this pilot study, we aimed to compare metabolite profiles of the whole serum and serum-derived exosomes in the context of differences between cancer patients and healthy controls as well as patients’ response to RT. Methods Serum samples were collected from 10 healthy volunteers and 10 patients with head and neck cancer, in the latter group samples were taken before and after the end of RT. Exosomes were isolated from serum by the size-exclusion chromatography. Metabolites were purified from the complete serum and exosomes using a methanol extraction and analyzed by the gas chromatography-mass spectrometry (GC-MS). Results An untargeted GC-MS-based approach allowed the detection of 182 and 46 metabolites in serum and exosomes, respectively (33 compounds were present in both types of specimens). The unsupervised analyses revealed that metabolite profiles of the whole serum but not serum-derived exosomes enabled the separation of all 3 groups of samples. There were 27 compounds whose serum levels were markedly different (large effect size) between control and cancer samples and 12 compounds whose serum levels were markedly different between cancer pre-RT and post-RT samples. On the other hand, only 4 metabolites present in exosomes showed markedly different levels between cancer and control samples. Noteworthy, metabolites that differentiated cancer and control samples, either serum or exosomes, were associated with energy metabolism pathways. Serum metabolites affected by RT were associated with pathways involved in the metabolism of amino acids, sugars, lipids, and nucleotides. Conclusions Metabolite profile of serum-derived exosomes is less complex than that of the complete serum. However, cancer-specific features of energy metabolism could be detected in both types of specimens. On the other hand, in contrast to RT-induced changes observed in serum metabolome, this pilot study did not reveal a specific radiation-related pattern of exosome metabolites.

NUTRITION ASSESSMENT IN HEAD AND NECK CANCER PATIENTS PRE and POST CHEMORADIOTHERAPY.

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Piyush Kumar ◽  
Bhavya P Pateneedi ◽  
Dharam P Singh ◽  
Arvind K Chauhan
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Nutritional Risk ◽  
Total Leukocyte Count ◽  
Risk Indicator ◽  
Anthropometric Parameters ◽  
Skin Fold Thickness ◽  
Before And After

INTRODUCTION: Head and neck cancer patients are frequently malnourished at the time of diagnosis and prior to the beginning of treatment. Deterioration of the nutritional status results in an increase in chemo radiotherapy related toxicity and this may increase the prolonged treatment time, which has been associated with poor clinical outcome. The present study aims to do nutritional assessment before and after chemo radiotherapy in head and neck cancer patients. MATERIAL AND METHODS: The present study was undertaken at the Department of Radiation Oncology, Shri Ram Murti Smarak Institute of Medical Sciences, Bareilly. In this study, 50 patients of Head and neck tumours were enrolled and their nutrition was assessed before and after chemoradiotherapy. Nutrition assessment was done using different laboratory parameters like haemoglobin, total leukocyte count, blood urea, serum creatinine and serum bilirubin. Anthropometric parameters used are Body mass index, Skin fold thickness, and Mid-arm circumference. Nutritional risk indicator and PG-SGA score is measured before and after chemoradiotherapy. All the parameters were assessed and analysed using different statistical tests- Chi-square test, Fisher Exact test and paired t test.RESULTS: Haemoglobin decrease was statistically significant during treatment (p less than 0.001) and the decrease in total leukocyte count during treatment was showing trend towards significance (p value-0.056). There was deterioration in other parameters like blood urea, serum creatinine and serum bilirubin but was not statistically significant. Anthropometric parameters- Body mass index, mid-arm circumference and skin fold thickness and percent body fat showed a significant change (p less than 0.00001). Nutritional risk indicator and PG-SGA class has decreased for majority of patients during treatment, the change is statistically significant (p less than 0.00001 and p=0.0251) respectively.CONCLUSION: The nutrition has important role to play in the management of head and neck cancers by chemo radiotherapy. It helps to reduce the complications and improve the tolerance of chemo radiotherapy, thus avoiding treatment breaks which may lead to failure of treatment.

scholarly journals An assessment of Oxford Nanopore sequencing for human gut metagenome profiling: A pilot study of head and neck cancer patients

2019 ◽  
Vol 166 ◽  
pp. 105739 ◽  
Author(s):  
Thidathip Wongsurawat ◽  
Mayumi Nakagawa ◽  
Omar Atiq ◽  
Hannah N. Coleman ◽  
Piroon Jenjaroenpun ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Pilot Study ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Nanopore Sequencing ◽  
Human Gut ◽  
Oxford Nanopore ◽  
Gut Metagenome

Prospective, Longitudinal Quality-of-Life Study of Patients With Head and Neck Cancer: A Feasibility Study Including the EORTC QLQ-C30

1997 ◽  
Vol 116 (6) ◽  
pp. 666-673 ◽  
Author(s):  
Eva Hammerlid ◽  
Kristin Bjordal ◽  
Marianne Ahlner-ELMQVIST ◽  
Magnus Jannert ◽  
Stein Kaasa ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Head And Neck Cancer ◽  
Psychological Distress ◽  
Pilot Study ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Eortc Qlq C30 ◽  
Eortc Qlq

Despite modern advances in the treatment of head and neck cancer, the survival rate fails to improve. Considering the different treatment modalities involved, quality of life has been thought of as an additional end point criterion for use in clinical trials. A Nordic protocol to measure the quality of life of head and neck cancer patients before, during, and after treatment was established. Before the study, a pilot study was done with this protocol. The main purpose of this pilot study was to find out whether this cancer population would answer quality-pf-life questionnaires repeatedly (six times) over a 1-year period and whether the chosen questionnaires—a core questionnaire (European Organisation for Research and Treatment of Cancer Core Quality of Life Questionnaire (EORTC QLQ-C30)), a tumor-specific questionnaire, and a psychological distress measure (Hospital Anxiety and Depression scale (HAD))—were sensitive for changes to functions and symptoms during the study year. The results presented in this article all refer to the pilot study. Forty-eight consecutive patients agreed to participate in the study. The most common tumor locations were the oral cavity (17) and the larynx (12). Almost all patients received combined treatment: 45 of 48 radiation therapy, 18 of 48 chemotherapy, and 17 of 48 surgery. After the primary treatment, 40 patients had complete tumor remission. Four of the 48 patients did not answer any questionnaires and were therefore excluded from the study. Of the remaining 44 patients, 3 died during the study year, and another 6 withdrew for various reasons. Thirty-five (85%) of the 41 patients alive at the 1-year follow-up answered all six questionnaires and thus completed the study. Mailed questionnaires were used throughout the study. All questionnaires were well accepted and found to be sensitive to changes during the study year. The greatest variability was found for symptoms and functions related specifically to head and neck cancer. The symptoms were swallowing difficulties, hoarse voice, sore mouth, dry mouth, and problems with taste. They all showed the same pattern, with an increase of symptoms during and just after finishing the treatment. The HAD scale revealed a high level of psychological distress, with 21% probable cases of psychiatric morbidity at diagnosis. In conclusion, it was shown that the study design and questionnaires were feasible for the forthcoming prospective quality-of-life assessment of Swedish and Norwegian head and neck cancer patients.

Improvement of a Delayed Swallowing Reflex Following Treatment for Advanced Head and Neck Cancer

2020 ◽  
Author(s):  
Akira Ohkoshi ◽  
Kengo Kato ◽  
Takenori Ogawa ◽  
Ayako Nakanome ◽  
Ryo Ishii ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Retrospective Study ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Advanced Head ◽  
Silent Aspiration ◽  
Swallowing Function ◽  
Before And After ◽  
Swallowing Reflex

Abstract Background: The latency of the swallowing reflex is an important factor causing dysphagia in head and neck cancer patients. Although there are many reports comparing voluntary swallowing function before and after treatment, few studies have focused on the latency of the swallowing reflex, which is a risk factor for pneumonia due to silent aspiration. The aim of this retrospective study was to clarify the changes in the latency of the swallowing reflex before and after treatment. Methods: The latency of the swallowing reflex was quantified using the time from the injection of 1 ml of distilled water into the pharynx through a nasal catheter to the onset of swallowing. Results: The latency time of the swallowing reflex was significantly decreased 3 months after treatment compared to before treatment. A significant reduction was also observed in patients with pharyngeal cancer who underwent chemoradiation therapy. Conclusions: This retrospective study showed that a delayed swallowing reflex improved with treatment in advanced head and neck cancer patients.

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