Characterization of Molecular Species and Anti-Inflammatory Activity of Purified Phospholipids from Antarctic Krill Oil

The phospholipids (PLs) from Antarctic krill oil were purified (>97.2%) using adsorption column chromatography. Forty-nine PL molecular species were characterized by ultrahigh-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). Most of molecular species contained eicosapentaenoic acid (EPA, 20:5), docosahexaenoic acid (DHA, 22:6), docosapentaenoic acid (DPA, 22:5), and arachidonic acid (AA, 20:4). Notably, a special species PC (20:5/22:6) (1298.17 nmol/g) and many ether PLs were detected. The Antarctic krill PL liposome (IC50 = 0.108 mg/mL) showed better anti-inflammatory activity than crude Antarctic krill oil (IC50 = 0.446 mg/mL). It could block NF-κB signaling pathway via suppression of IκB-α degradation and p65 activation and dose-dependently reduce the cellular content of inflammatory mediators including nitric oxide (NO), reactive oxygen species (ROS), and inflammatory cytokines in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In addition, it can suppress carrageenan-induced mouse paw swelling. Results from the present study could provide a reference for better evaluation of nutritional and medicinal values of Antarctic krill oil.

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Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro

Steroids ◽

10.1016/j.steroids.2021.108830 ◽

2021 ◽

pp. 108830

Author(s):

Xiaorui Cai ◽

Fei Sha ◽

Chuanyi Zhao ◽

Zhiwei Zheng ◽

Shulin Zhao ◽

...

Keyword(s):

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Anti Inflammatory

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Effects of Antarctic krill oil on lipid and glucose metabolism in C57BL/6J mice fed with high fat diet

Lipids in Health and Disease ◽

10.1186/s12944-017-0601-8 ◽

2017 ◽

Vol 16 (1) ◽

Cited By ~ 9

Author(s):

Dewei Sun ◽

Liang Zhang ◽

Hongjian Chen ◽

Rong Feng ◽

Peirang Cao ◽

...

Keyword(s):

Glucose Metabolism ◽

High Fat Diet ◽

Antarctic Krill ◽

Krill Oil ◽

High Fat ◽

Antarctic Krill Oil

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Enzymatically Decomposed Antarctic Krill Oil Inhibits Atherosclerosis by Suppressing the Vascular Endothelial Inflammation

Food Supplements and Biomaterials for Health ◽

10.52361/fsbh.2021.1.e9 ◽

2021 ◽

Vol 1 (1) ◽

Author(s):

Min Yu ◽

Hee Joon Jun ◽

Youngbae Son ◽

Cheoljin Park ◽

Jin Tae Hong ◽

...

Keyword(s):

Antarctic Krill ◽

Vascular Endothelial ◽

Krill Oil ◽

Endothelial Inflammation ◽

Antarctic Krill Oil

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RETRACTED: Anti-inflammatory Activity of 4-Arylcoumarins from EndophyticStreptomyces aureofaciensCMUAc130 in Murine Macrophage RAW 264.7 Cells

Immunological Investigations ◽

10.1080/08820130600992115 ◽

2007 ◽

Vol 36 (2) ◽

pp. 203-211 ◽

Cited By ~ 10

Author(s):

Thongchai Taechowisan ◽

Pittaya Tuntiwachwuttikul ◽

Chunhua Lu ◽

Yuemao Shen ◽

Saisamorn Lumyong ◽

...

Keyword(s):

Inflammatory Activity ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Murine Macrophage ◽

Anti Inflammatory

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Preparation of Antarctic Krill Oil Emulsion and Its Stability under Catalase Treatment

Foods ◽

10.3390/foods10112797 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2797

Author(s):

Zhenxiao Zheng ◽

Kai Zhu ◽

Zhiyuan Dai

Keyword(s):

Enzyme Activity ◽

Lipid Oxidation ◽

Antarctic Krill ◽

Effective Means ◽

Thiobarbituric Acid ◽

Tween 80 ◽

Iron Porphyrin ◽

Krill Oil ◽

Oil Emulsion ◽

Antarctic Krill Oil

Making Antarctic krill oil into emulsion is a good way to utilize Antarctic krill, but proliferation of microorganisms cannot be ignored. H2O2 is widely used in the sterilization of liquid food since its decomposition products are environmentally friendly, although residual H2O2 should be removed for food safety. Adding catalase (CAT) is an effective means to do this. However, the enzyme activity center of CAT is the iron porphyrin group, which has the risk of accelerating lipid oxidation in the oil emulsion. Therefore, we hypothesized that CAT might not be suitable for the removal of H2O2 in Antarctic krill oil emulsion. In this paper, Antarctic krill oil emulsion was prepared, and then the effect of CAT on the emulsion was studied through visual observation, microscopic morphology observation, turbidity and stability, particle size, and ζ-potential; finally, the mechanism of CAT destroying the emulsion was explored from the perspective of lipid oxidation. The results showed that a stable Antarctic krill emulsion was prepared using Tween-80 as the emulsifier, with the oil concentration of 1% (v/v) and the ratio of surfactant to oil phase of 1:5 (v/v). The emulsion treated with CAT had undergone demulsification, stratification, and coagulation after 2 days of incubation, while the emulsion treated with superoxide dismutase (SOD) and bovine serum albumin (BSA) changed little. In addition, the thiobarbituric acid reactive substances (TBARS) value and the content of hydroxyl radicals in the CAT group increased significantly. The preliminary research results indicated that the effect of CAT on the emulsion related to the lipid oxidation caused by the iron porphyrin group at the center of the enzyme activity. All these results indicated that CAT was not suitable for the removal of residual H2O2 in Antarctic krill oil emulsion. Moreover, it is helpful to avoid the contact of Antarctic krill oil emulsion and CAT during the processing of the krill.

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