scholarly journals Multi-Modal Mass Spectrometric Imaging of Uveal Melanoma

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 560
Author(s):  
Laura M. Cole ◽  
Joshua Handley ◽  
Emmanuelle Claude ◽  
Catherine J. Duckett ◽  
Hardeep S. Mudhar ◽  
...  

Matrix assisted laser desorption ionisation mass spectrometry imaging (MALDI-MSI), was used to obtain images of lipids and metabolite distribution in formalin fixed and embedded in paraffin (FFPE) whole eye sections containing primary uveal melanomas (UM). Using this technique, it was possible to obtain images of lysophosphatidylcholine (LPC) type lipid distribution that highlighted the tumour regions. Laser ablation inductively coupled plasma mass spectrometry images (LA-ICP-MS) performed on UM sections showed increases in copper within the tumour periphery and intratumoural zinc in tissue from patients with poor prognosis. These preliminary data indicate that multi-modal MSI has the potential to provide insights into the role of trace metals and cancer metastasis.

Metallomics ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1974-1983 ◽  
Author(s):  
Barbara R. Cardoso ◽  
Katherine Ganio ◽  
Blaine R. Roberts

Electrospray ionisation mass spectrometry (ESI-MS) is an alternative to inductively coupled plasma mass spectrometry (ICP-MS) to better understand selenium biochemistry.


2013 ◽  
Vol 1 (1) ◽  
pp. 8
Author(s):  
Lisa C. Niziolek

Although significant historical research has been done on traditional Southeast Asian kingdoms and chiefdoms, little archaeological work has been undertaken on changes in the economic systems of pre-colonial maritime societies in Asia, especially on the role of specialised craft production in the development of pre-modern complex societies. This project examines changes in the organisation of earthenware production in the prehispanic coastal polity of Tanjay in the Philippines (A.D. 500-1600). More than 250 earthenware pieces from six archaeological sites from the Tanjay region were analysed using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) at Chicago’s Field Museum. Ceramic samples were drawn from two residential zones in central Tanjay, an elite neighborhood and a non-elite area; two secondary settlements located several kilometers upriver; an upland, swidden farming site; and a contemporaneous, and likely competing, coastal polity 40 km down the coast from Tanjay. Initially, it was expected that changes in the pattern of earthenware production in the Tanjay region would favour one scenario or the other – either continued production at dispersed, local sites or increased specialised and centralised production. So far, however, the preliminary ceramic compositional evidence indicates that both scenarios seem to have been taking place during the centuries prior to Spanish contact. Ceramic production appears to have continued on a local level, with potters from each site making pottery to be used by nearby inhabitants, but there also is evidence that sites, such as the elite Tanjay neighborhood, began to make ceramics expressly for local consumption by elites and for foreign trade.


2002 ◽  
Vol 8 (3) ◽  
pp. 213-232 ◽  
Author(s):  
Alexander A. Sysoev ◽  
Alexey A. Sysoev

At the beginning of the age of laser-ionisation mass spectrometry (LIMS) increasing numbers of publications were observed. However, later the method began to run into obstacles associated with poor reproducibility of analysis and large variations in elemental sensitivities so that the wide interest of the scientific community in the method faded away. However, the results described here show that the current knowledge of laser plasma processes, together with modern technical solutions to ion separation and quantification with time-of-flight (ToF) mass spectrometry, allow one to overcome the above-mentioned obstacles in LIMS. Thus, the performance in direct-sampling solid analysis demonstrated by the LAMAS-10M instrument is similar to that typically obtained by laser ablation/inductively-coupled plasma mass spectrometry (LA-ICP-MS) and glow-discharge mass spectrometry (GD-MS) methods. At the same time, there are additional advantages, including compactness of the instrument, absence of the need for expensive consumables and freedom from mass line interferences. Direct-sampling elemental LIMS is discussed as a promising alternative to LA-ICP-MS and GD-MS. Existing and prospective approaches to designing direct-sampling laser-ionisation mass spectrometers are theoretically justified. Factors affecting the main performance criteria, such as reproducibility, correctness, variations of relative sensitivity factors, linear dynamic range and resolution are considered. The demonstrated reproducibility, resolution, low-ppb limit of detection and one order-of-magnitude variation in elemental sensitivity are not the limit for direct-sampling laser-ionisation mass spectrometry of solid samples. Ways of improving LI-ToF-MS instrumental performance are discussed and theoretically justified.


2019 ◽  
Author(s):  
Ingo Strenge ◽  
Carsten Engelhard

<p>The article demonstrates the importance of using a suitable approach to compensate for dead time relate count losses (a certain measurement artefact) whenever short, but potentially strong transient signals are to be analysed using inductively coupled plasma mass spectrometry (ICP-MS). Findings strongly support the theory that inadequate time resolution, and therefore insufficient compensation for these count losses, is one of the main reasons for size underestimation observed when analysing inorganic nanoparticles using ICP-MS, a topic still controversially discussed.</p>


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