scholarly journals Molecular Identification and Targeted Quantitative Analysis of Medicinal Materials from Uncaria Species by DNA Barcoding and LC-MS/MS

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 175 ◽  
Author(s):  
Shugen Wei ◽  
Zuliang Luo ◽  
Shengrong Cui ◽  
Jing Qiao ◽  
Zhonglian Zhang ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Dna Barcoding ◽  
Evaluation System ◽  
Accurate Determination ◽  
Chemical Compositions ◽  
Therapeutic Effects ◽  
Chemical Analyses ◽  
Bioactive Constituents

The genus Uncaria is an important source of traditional Chinese medicines with multiple therapeutic effects. The identification of the correct species and accurate determination of the contents of bioactive constituents are important for quality control of Uncaria medicinal materials. Here, an integrated evaluation system based on DNA barcoding for species identification and quantitative analysis by LC-MS/MS has been established. DNA barcoding based on the ITS2 barcode region showed sufficient discriminatory power to precisely identify 24 samples from seven Uncaria species. The length of the 24 ITS2 sequences of Uncaria samples is 227 bp, and 17 variation sites were detected. Additionally, the results of qualitative and quantitative chemical analyses by LC-MS/MS indicated that the chemical compositions of all Uncaria samples were similar; while their contents of targeted alkaloids in samples from different species and origin areas were different. The contents of rhynchophylline (RC) and isorhynchophylline (IRC) were 2.9–1612 mg/kg and 2.60–1299 mg/kg in all tested samples, respectively. This study concludes that DNA barcoding should be used as the first screening step for Uncaria medicinal materials. Then, integration of DNA barcoding with chemical analyses should be applied in quality control of Uncaria medicinal materials in the medicinal industry.

New Determination Method of Two Bioactive Saponins in Lysimachia capillipes Hemsl. by Quantitative Analysis of Multi-Components by Single-Marker

2021 ◽  
Author(s):  
Xiaoyong Zhang ◽  
Xuezhao Chen ◽  
Juan Jin ◽  
Minghua Gong ◽  
Qiang He ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
High Performance ◽  
Reference Standards ◽  
Bioactive Components ◽  
Evaporative Light Scattering Detector ◽  
Evaporative Light Scattering ◽  
External Standard ◽  
Single Marker

Abstract Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.

The chemistry and cell parameters of omphacites and related pyroxenes

1969 ◽  
Vol 37 (285) ◽  
pp. 61-74 ◽  
Author(s):  
A. D. Edgar ◽  
A. Mottana ◽  
N. D. Macrae
Keyword(s):  
Powder Diffraction ◽  
Electron Microprobe ◽  
Graphical Representation ◽  
Chemical Compositions ◽  
Chemical Analyses ◽  
X Ray ◽  
Cell Parameters ◽  
Cell Dimensions ◽  
Approximate Estimation

SummaryIn an attempt to correlate the chemical compositions and cell sizes of omphacites and related pyroxenes, the cell dimensions of fifty-five analysed pyroxenes have been determined, or taken from the literature. Twenty-two of the chemical analyses are new, nineteen of them being done by electron microprobe. Approximately two-thirds of the total number of analyses may be considered first class, the remainder are of doubtful or unknown quality. Cell parameters, determined by X-ray powder diffraction methods, have errors of 0·1 % for the majority of samples, although for some samples taken from the literature errors are unknown.The majority of methods of recalculating omphacite analyses into their end-member molecules are unsuitable for correlation of cell constants with chemistry, mainly due to the impossibility of graphical representation of more than three end-member molecules, and to the non-stoichiometry of these molecules. Using a modification of Tröger's (1962) method of recalculating chloromelanite analyses the present analyses have been recalculated into the diopside-jadeite-acmite and diopside-jadeite-hedenbergite molecules and compared with their determined cell parameters. Because of the gradations in all parameters between these end-member molecules, determination of compositions based on the cell parameters (a, b, c, vol, or β) can only be made within wide limits. However, using a method of projection of compositions from the acmite and hedenbergite apices to the diopside-jadeite join the ratios of diopside to jadeite can be determined for most samples to within ±5 mol%. As there are the most important constituents of most omphacites, this method permits an approximate estimation of omphacite compositions. From a knowledge of the cell sizes of the omphacite a rough indication of the conditions of formation of its host rock may also be obtained.

scholarly journals Simultaneous Determination of Four Major Constituents of Semen Vaccariae Using HPLC-DAD

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Haijiang Zhang ◽  
Wei Yao ◽  
Yunyun Chen ◽  
Peipei He ◽  
Yao Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Gradient Elution ◽  
Hplc Method ◽  
Frying Temperature ◽  
Simultaneous Quantification ◽  
Frying Process

A simple and reliable HPLC method was developed and validated for the simultaneous quantification of four major constituents in Semen Vaccariae. The chromatographic separation was performed on an Agilent Zorbax SB-C18 column with gradient elution using methanol and water. The calibration curves showed good linearity of R2 > 0.9999 with LOQs (S/N = 10) of 0.20–1.16 μg/mL. The precision was evaluated by intra- and inter-day assays and R.S.D. values were less than 2.09%. The recovery rates were between 97.0% and 105.0%. The developed method was applied to the quantitative analysis of Semen Vaccariae and its stir-fried products. During the stir-frying process, vaccarin degraded and yielded isovitexin-2″- O-arabinoside. The preferable stir-frying temperature is around 120°C. The developed HPLC method can be applied to the quality control of crude and stir-fried Semen Vaccariae.

Investigation and Analytical Applications of the Reaction of N1-Methylnicotinamide and Active Methylene Containing Drugs

2020 ◽  
Vol 17 ◽  
Author(s):  
Heba A. Elsebaie ◽  
Mervat H. Elhamamsy ◽  
Khaled M. Elokely ◽  
Mohammed A. Eldawy
Keyword(s):  
Quality Control ◽  
Free Energy ◽  
Spectral Analysis ◽  
Quantitative Analysis ◽  
Geometry Optimization ◽  
Fluorescent Product ◽  
Spectrofluorometric Method ◽  
Active Methylene ◽  
Methylene Group

Background: The reaction between N1-methylnicotinamide iodide (NMNI) and the active methylene group containing compounds yields a fluorescent product, which can be used in the quantitative determination of certain drugs. Objective: The objective is to develop, adapt and validate a simple spectrofluorometric method for the quantitative analysis of modafinil 1, budesonide 2, allicin 3 and florfenicol 4. Results: A C4 cyclization pathway was confirmed for the formation of the fluorophore. The spectrofluorometric method showed good linearity (R2= 0.996-0.999) over concentrations of 1-50 ng/mL, 0.5-5 ng/mL, 60-150 pg/mL, and 1-15 ng/mL of the standard solutions of 1, 2, 3, and 4, respectively. Methods: Spectral analysis (elemental analysis, 1H, 13C NMR, MS), computational methods (geometry optimization, Gibbs free energy and pKa calculations) and spectrofluorometric approaches were conducted. Conclusion: The proposed method is simple and suitable for quality control and assurance studies

scholarly journals Analysis of Flavonoids and Iridoids in Vitex Negundo by HPLC-PDA and Method Validation

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800
Author(s):  
Somendu K. Roy ◽  
Khemraj Bairwa ◽  
Jagdeep Grover ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Method Validation ◽  
Hplc Method ◽  
Vitex Negundo ◽  
Good Recovery ◽  
Bioactive Constituents ◽  
Ich Guidelines ◽  
Validation Parameters

The leaves of Vitex negundo have been reported to contain various bioactive constituents including iridoids and flavonoids. This is the first report on the simultaneous determination of iridoids and flavonoids by HPLC in three different samples of V. negundo leaves collected from three regions of India. Separation of iridoids and flavonoids was accomplished by HPLC and further elaborated for their quantification in V. negundo leaves using a C-18 column with detection at 254 and 330 nm, respectively. The developed HPLC method showed good linearity (r2≥0.999), high precision (RSD<5%) and a good recovery (99.3–103.0%) of the compounds. All the validation parameters of the developed HPLC were found to be within the permissible limits according to the ICH guidelines. The developed method was robust, accurate and reliable for the quality control of V. negundo leaves.

scholarly journals Quality Control of the Fuzi Lizhong Pill Through Simultaneous Determination of 16 Major Bioactive Constituents by RRLC–MS-MS

2018 ◽  
Vol 56 (6) ◽  
pp. 541-554 ◽  
Author(s):  
Yun-zhuo Dong ◽  
Zhen-li Liu ◽  
Yuan-yan Liu ◽  
Zhi-qian Song ◽  
Na Guo ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Bioactive Constituents

scholarly journals DNA barcoding reveals the diversity of sharks in Guyana coastal markets

2017 ◽  
Vol 15 (4) ◽  
Author(s):  
Matthew A. Kolmann ◽  
Ahmed A. Elbassiouny ◽  
Elford A. Liverpool ◽  
Nathan R. Lovejoy
Keyword(s):  
Dna Barcoding ◽  
Accurate Determination ◽  
Total Sample ◽  
Artisanal Fishing ◽  
Species Identity ◽  
Sustainable Fisheries ◽  
International Union ◽  
Accurate Identification ◽  
Fundamental Challenge

ABSTRACT A fundamental challenge for both sustainable fisheries and biodiversity protection in the Neotropics is the accurate determination of species identity. The biodiversity of the coastal sharks of Guyana is poorly understood, but these species are subject to both artisanal fishing as well as harvesting by industrialized offshore fleets. To determine what species of sharks are frequently caught and consumed along the coastline of Guyana, we used DNA barcoding to identify market specimens. We sequenced the mitochondrial co1 gene for 132 samples collected from six markets, and compared our sequences to those available in the Barcode of Life Database (BOLD) and GenBank. Nearly 30% of the total sample diversity was represented by two species of Hammerhead Sharks (Sphyrna mokarran and S. lewini), both listed as Endangered by the International Union for Conservation of Nature (IUCN). Other significant portions of the samples included Sharpnose Sharks (23% - Rhizoprionodon spp.), considered Vulnerable in Brazilian waters due to unregulated gillnet fisheries, and the Smalltail Shark (17% - Carcharhinus porosus). We found that barcoding provides efficient and accurate identification of market specimens in Guyana, making this study the first in over thirty years to address Guyana’s coastal shark biodiversity.

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