Preparative Separation of Monoterpenoid Indole Alkaloid Epimers from Ervatamia yunnanensis Tsiang by pH-Zone-Refining Counter-Current Chromatography Combined with Preparative High-Performance Liquid Chromatography
Keyword(s):
2D Nmr
◽
An effective method was developed for the preparative separation and purification of monoterpenoid indole alkaloid epimers from Ervatamia yunnanensis Tsiang using a combination of pH-zone-refining counter-current chromatography and preparative high-performance liquid chromatography. With this method, two pairs of MIA epimers including ervatamine (72 mg, 1), 20-epi-ervatamine (27 mg, 4), dregamine (95 mg, 2), tabernaemontanine (129 mg, 3), along with two MIAs, apparicine (112 mg, 5) and isovoacangine (15 mg, 6), were successfully purified from 2.1 g crude extract of E. yunnanensis, each with a purity of over 95% as determined by HPLC. The structures of the MIAs were identified by ESI-MS, 1D, and 2D NMR.
2009 ◽
Vol 3
(4)
◽
pp. 375-382
◽
2014 ◽
Vol 37
(7)
◽
pp. 974-987
◽
2016 ◽
Vol 79
(9)
◽
pp. 880-889
◽
2019 ◽
Vol 42
(6)
◽
pp. 1194-1201
◽
2014 ◽
Vol 49
(4)
◽
pp. 594-600
◽
1986 ◽
Vol 153
(2)
◽
pp. 230-234
◽
1997 ◽
Vol 763
(1-2)
◽
pp. 65-70
◽
1991 ◽
Vol 539
(1)
◽
pp. 15-23
◽
2017 ◽
Vol 1511
◽
pp. 143-148
◽