scholarly journals Amelioration of Hyperglycemia-Induced Nephropathy by 3,3′-Diindolylmethane in Diabetic Mice

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4474
Author(s):  
Kyeong-Mi Choi ◽  
Hwan-Soo Yoo

Type 1 diabetes mellitus (insulin-dependent diabetes) is characterized by hyperglycemia caused by an insulin deficiency. Diabetic nephropathy is a major complication of hyperglycemia. 3,3′-diindolylmethane (DIM)-a natural compound produced from indole-3-carbinol, found in cruciferous vegetables-enhances glucose uptake by increasing the activation of the insulin signaling pathway in 3T3-L1 adipocytes. In this study, we investigated whether DIM could improve insulin-dependent diabetes and nephropathy in streptozotocin (STZ)-induced diabetic mice. In mice, STZ induced hyperglycemia, hunger, thirst, and abnormally increased kidney weight and serum creatinine, which is a renal functional parameter. DIM decreased STZ-increased high blood glucose levels and food and water intake in diabetic mice. DIM also improved diabetic nephropathy by inhibiting the expression of PKC-α, the marker of albuminuria, and TGF-β1, an indicator of renal hypertrophy, in diabetic mice. Our findings suggest that DIM may ameliorate hyperglycemia and diabetic nephropathy through the inhibition of PKC-α and TGF-β1 signaling.

2020 ◽  
Vol 10 (17) ◽  
pp. 6082
Author(s):  
Zulfa Nailul Ilmi ◽  
Pugar Arga Cristina Wulandari ◽  
Saikhu Akhmad Husen ◽  
Dwi Winarni ◽  
Mochammad Amin Alamsjah ◽  
...  

Diabetes mellitus is a group of metabolic disorders characterized by high blood-glucose levels over a prolonged period that causes complications when an open wound is present. Alginate is an antioxidant and a good absorbent of exudates. Okra fruit contains flavonoids that can act as antioxidants. The antioxidant properties of extracts combination reduce blood-glucose levels significantly to accelerate the activities of wound-healing processes on diabetic mice. Alginate was characterized by Size Exclusion Chromatography-Multiple Angle Laser Light Scattering (SEC-MALLS), thermal stability and Proton Nuclear Magnetic Resonance (1H-NMR). The evaluation of wound healing on 36 male mice were divided into 12 groups including normal control (NC), diabetics control (DC), alginate (DA) and alginate–okra (DAO) groups in three different times by histopathology test on skin tissue. The results of SEC-MALLS analysis showed that alginate as single and homogeneous polysaccharide. The 1H-NMR spectrum showed that the mannuronate/guluronate ratio of the used alginate was 0.91. Alginate, okra fruit extract and their combination were classified as moderate and strong antioxidants. The numbers of fibrocytes, fibroblasts, collagen densities had significantly increased from three to seven days. In contrast, wound width, neutrophil, macrophages had significantly decreased at 14 days. The administration of extracts combination increased the re-epithelization of the wound area and wound-healing process on diabetic mice.


1987 ◽  
Vol 9 (5) ◽  
pp. 155-162
Author(s):  
John W. Reynolds

1. All foods should be labeled to indicate the calories, sources of the calories, the mineral and vitamin content, the types and amounts of carbohydrates, the amount of cholesterol and amounts and types of fatty acids, and the amount and type of artificial sweetener, if any. 2. A long-term evaluation of the exchange system in the dietary instruction of patients with insulin-depedent diabetes mellitus and their families should be carried out. 3. General guidelines for meal plans for youngsters with diabetes should allow for flexibility and variety in food selection so that they account for individual differences as well as being applicable to different socio-economic, cultural, and ethnic groups. 4. The distribution of calories in the diet should be: 50% to 60% carbohydrate, 15% to 20% protein, and 20% to 25% fat. 5. Nutrition education of the patient with insulin-dependent diabetes mellitus and of the patient's family about the principles of good nutrition, the importance of a balanced meal plan, and the rationale for food prohibitions should be a critical part of diabetes care. 6. Nutritional assessment should include plotting height and weight measurements on standard growth grids at regular intervals. 7. Scientifically sound cross-over studies of the differences between "simple" and "complex" carbohydrates need to be carried out on children with insulin-dependent diabetes mellitus. 8. Before optimal and safe use of fiber in therapy of children is possible, further studies are needed of the convenience and palatability of meals with currently available fiber, the use of "medicated foods" to which specific types of fiber have been added, and the extent of trace element and mineral binding by various fibers. 9. More studies are needed concerning the effects of food form, nutrient-nutrient interactions, and non-nutritive constituents of food on the glycemic effect of a given food. 10. Fructose and sorbitol may be used in limited amounts as parts of an otherwise nutritious and well-balanced meal plan, but the usefulness of their chronic ingestion has not been established. 11. Fructose and sorbitol are not substitutes for artificial noncaloric sweeteners and should not be used in the belief that they are of use in weight control. 12. Aspartame contains no calories and has no apparent risks. However, consumption of large amounts of granulated aspartame, which contains a lactose or dextrose carrier, could affect blood glucose levels. The use of cyclamate and saccharin by children with insulin-dependent diabetes mellitus should be limited pending further review. 13. The use of combinations of artificial sweeteners is reasonable to limit the risks associated with any one sweetener. 14. The medical profession and patients with insulin-dependent diabetes mellitus should be made aware that the disease is a disorder of lipid metabolism as well as carbohydrate metabolism. 15. Cholesterol and saturated fat intake should be limited and the total calories from fat should be reduced to 20% to 25% of the caloric intake. 16. Patients with insulin-dependent diabetes mellitus should have at least yearly monitoring of their fasting serum total and high-density lipoprotein cholesterol levels and the triglyceride levels. 17. The patient with insulin-dependent diabetes mellitus should be taught that, in addition to control of the dietary lipid intake, limitation of the high rate of cardiovascular complications associated with insulin-dependent diabetes mellitus depends on excellent control of blood glucose levels, age-appropriate aerobic exercise, and avoidance of the use of tobacco in any form. 18. Blood pressure should be monitored every 6 to 12 months. It is critical that hypertension be diagnosed and treated early. 19. Young patients with insulin-dependent diabetes mellitus, and their families, should be educated in a prudent approach to the use of dietary salt, with little use of added salt and a choice of foods without excessive sodium content. However, supplemental sodium chloride may be warranted when there is glycosuria in a newly diagnosed or poorly controlled patient. 20. Insulin-dependent diabetes mellitus does not result in increased dietary requirements for iron, magnesium, zinc, selenium, chromium, or other trace minerals or vitamins and, therefore, should not be an indication per se for mineral or vitamin supplements. 21. Nutritionists and dietitians with expertise in the treatment of childhood and adolescent diabetes should be recognized as valuable members of a multidisciplinary diabetes health care team. The role of such a nutritionist or dietitian includes an initial assessment and then later adjustment of meal plans to be consistent with the patient's growth, development, changes in activity level, and appearance of complications. 22. Families should be advised to follow principles of good nutrition in their own meal planning as a way to increase dietary compliance by their children with insulin-dependent diabetes mellitus. Flexibility in meal plans should be encouraged as a way to maximize dietary compliance.


1994 ◽  
Vol 20 (1) ◽  
pp. 41-44 ◽  
Author(s):  
Lila Laux

Patients with type II, non-insulin-dependent diabetes mellitus (NIDDM) typically are middle-aged or older and often have diabetic retinopathy. The visual acuity of these patients also is likely to be reduced beyond what is normally associated with age. Because Medicaid does not reimburse NIDDM patierzts for a blood glucose meter unless they are insulin-dependent, many low-income diabetes patients are required to monitor their blood glucose levels by visually comparing the color spot on the blood glucose test strip with a series of standard color blocks. Unless patients can accurately assess their blood glucose levels by visual interpretation of the test strips, they will have difficulty maintaining adequate glycemic control. In this study, 60 nondiabetic adults, ages 20 to 78 years, were unable to adequately assess blood glucose levels visually, even under optimal lightirtg conditions. Younger adults made the same number of errors as older adults, and poor visual acuity and high blood glucose values were associated with more errors. These findings suggest that patients with poor visual acuity or those who read their blood glucose strips in less-than-adequate lighting will make even more errors than our test subjects.


1995 ◽  
Vol 23 (4) ◽  
pp. 294-298 ◽  
Author(s):  
S Okada ◽  
K Ishii ◽  
S Tanokuchi ◽  
H Hamada ◽  
K Ichiki ◽  
...  

Twenty patients with non-insulin-dependent diabetes mellitus who had been receiving appropriate dietary treatment for 3 months but whose glucose metabolism needed further improvement were treated with an α-glucosidase inhibitor. Treatment with the α-glucosidase inhibitor (0.6 mg/day) for 4 weeks, had no significant effect on blood glucose levels 2 h after breakfast or on glycosylated haemoglobin levels.


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