scholarly journals Development of Gas-Chromatographic Method for Simultaneous Determination of Cannabinoids and Terpenes in Hemp

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5872
Author(s):  
Jure Zekič ◽  
Mitja Križman
Keyword(s):  
Sample Preparation ◽  
Simultaneous Determination ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Linear Range ◽  
Extraction Solvent ◽  
Parallel Testing ◽  
Medium Polarity ◽  
Gas Chromatographic Method

An original gas chromatographic method has been developed for simultaneous determination of major terpenes and cannabinoids in plant samples and their extracts. The main issues to be addressed were the large differences in polarity and volatility between both groups of analytes, but also the need for an exhaustive decarboxylation of cannabinoid acidic forms. Sample preparation was minimised, also by avoiding any analyte derivatisation. Acetone was found to be the most appropriate extraction solvent. Successful chromatographic separation was achieved by using a medium polarity column. Limits of detection ranged from 120 to 260 ng/mL for terpenes and from 660 to 860 ng/mL for cannabinoids. Parallel testing proved the results for cannabinoids are comparable to those obtained from established HPLC methods. Despite very large differences in concentrations between both analyte groups, a linear range between 1 and 100 µg/mL for terpenes and between 10 and 1500 µg/mL for cannabinoids was determined.

scholarly journals Rapid Gas Chromatographic Method for Simultaneous Determination of Cholesterol and α-Tocopherol in Eggs

1998 ◽  
Vol 81 (6) ◽  
pp. 1177-1184 ◽  
Author(s):  
Nickos Botsoglou ◽  
Dimitrios Fletouris ◽  
Ioannis Psomas ◽  
Antonios Mantis
Keyword(s):  
Simultaneous Determination ◽  
Chromatographic Method ◽  
Labor Cost ◽  
Precision Data ◽  
Total Analysis Time ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Total Analysis ◽  
Gas Chromatographic Method

Abstract A new method was developed for simultaneous determination of cholesterol and α-tocopherol in eggs. It involves rapid and simple sample preparation accomplished in one tube and chromatographic separation that does not require derivatization of analytes. Total analysis time per sample is 40 min. Labor, cost, and use of hazardous chemicals are minimized. To ensure selectivity, accuracy, and precision, critical analytical parameters were investigated. Overall recoveries were 98.8 and 99.2% for cholesterol and α-tocopherol, respectively. Linearity was acceptable for both analytes (r = 0.9964 for cholesterol and 0.9996 for α-tocopherol) in the fortification range examined. Precision data based on within-day and between-days variation gave overall relative standard deviations of 2.0% for cholesterol and 7.0% for α-tocopherol.The method was applied successfully for quantitation of cholesterol and α-tocopherol in eggs.

Determination of fluoxetine and norfluoxetine in plasma by gas chromatography with electron-capture detection.

1982 ◽  
Vol 28 (10) ◽  
pp. 2100-2102 ◽  
Author(s):  
J F Nash ◽  
R J Bopp ◽  
R H Carmichael ◽  
K Z Farid ◽  
L Lemberger
Keyword(s):  
Gas Chromatography ◽  
Human Plasma ◽  
Electron Capture ◽  
Relative Error ◽  
Chromatographic Method ◽  
Linear Range ◽  
Electron Capture Detector ◽  
Electron Capture Detection ◽  
Gas Chromatographic Method

Abstract This gas-chromatographic method for assay of fluoxetine and norfluoxetine in human plasma involves extraction of the drugs and use of a 63Ni electron-capture detector. The linear range of detection is 25 to 800 micrograms/L for each drug. Overall precision (CV) in the concentration range of 10 to 100 micrograms/L for both drugs was approximately 10%. Accuracy (relative error) in the same concentration range was approximately +10%. None of the commonly prescribed antidepressants or tranquilizers that we tested interfere with the assay.

scholarly journals Simultaneous Determination of Patulin and Penicillic Acid in Grains by Gas Chromatographic Method

1974 ◽  
Vol 38 (6) ◽  
pp. 1259-1260 ◽  
Author(s):  
Toshimasa SUZUKI ◽  
Yoshinori FUJIMOTO ◽  
Yôji HOSHINO ◽  
Akio TANAKA
Keyword(s):  
Simultaneous Determination ◽  
Chromatographic Method ◽  
Penicillic Acid ◽  
Gas Chromatographic Method

Therapeutic Monitoring of Anticonvulsant Drugs: Gas-Chromatographic Simultaneous Determination of Primidone, Phenylethylmalonamide, Carbamazepine, and Diphenylhydantoin

1975 ◽  
Vol 21 (11) ◽  
pp. 1658-1662 ◽  
Author(s):  
Charles J Least ◽  
George F Johnson ◽  
Harvey M Solomon
Keyword(s):  
Simultaneous Determination ◽  
Standard Method ◽  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Normal Serum ◽  
Therapeutic Monitoring ◽  
The Mean ◽  
Gas Chromatographic Method

Abstract We describe a sensitive and precise gas-chromatographic method in which benzylmalonate methylester monoamide is used as the internal standard for the simultaneous determination of primidone, phenylethylmalonamide, carbamazepine, and diphenylhydantoin. The trimethylsilyl derivatives of the anticonvulsants are well separated from each other and from normal serum constituents. The lower limit of detection for each drug is 0.5 mg/liter when 1 ml of serum is analyzed. Withinrun precision (CV), established by analysis of 10 replicates, was as follows: primidone (5.4 mg/liter), 2.6%; phenylethylmalonamide (5.5 mg/liter), 1.6%; diphenylhydantoin (6.6 mg/liter), 3.8%; and carbamazepine (10.4 mg/liter), 3.2%. Fifty specimens were analyzed for primidone and 35 for diphenylhydantoin by a standard gas-chromatographic method involving on-column methylation and by the procedure we have developed. The mean value observed for primidone with the on-column alkylation procedure was 9.3 mg/liter and with our procedure was 9.6 mg/liter. When values for our assay were regressed against values for the standard method, the slope of the least-squares line was 0.936, the intercept was 1.00 mg/liter, and r was 0.939. The mean values observed for diphenylhydantoin by on-column methylation and with our procedure were both 12.6 mg/liter. When values for our assay were regressed against the standard method, the slope of the leastsquares line was 0.944, the intercept was 0.3 mg/liter, and r was 0.988

Antipyrine metabolism in man: simultaneous determination of norantipyrine and 4-hydroxyantipyrine in urine by gas chromatography

1980 ◽  
Vol 58 (1) ◽  
pp. 17-21 ◽  
Author(s):  
T. Inaba ◽  
N. E. Fischer
Keyword(s):  
Gas Chromatography ◽  
Simultaneous Determination ◽  
Chromatographic Method ◽  
Internal Standard ◽  
Antipyrine Metabolism ◽  
Drug Oxidation ◽  
Gas Chromatographic Method

A gas chromatographic method was developed to determine metabolites of antipyrine, norantipyrine (NORA), and 4-hydroxyantipyrine in urine using p-methylated NORA as internal standard. This method requires no derivatization and has ample sensitivity to determine these metabolites in urine after ingestion of antipyrine, a compound widely used as a hepatic probe of drug oxidation.

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