preparation procedure
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Anera Kazlagić ◽  
Francesco F. Russo ◽  
Jochen Vogl ◽  
Patrick Sturm ◽  
Dietmar Stephan ◽  

AbstractThe 87Sr/86Sr isotope ratio can, in principle, be used for provenancing of cement. However, while commercial cements consist of multiple components, no detailed investigation into their individual 87Sr/86Sr isotope ratios or their influence on the integral 87Sr/86Sr isotope ratio of the resulting cement was conducted previously. Therefore, the present study aimed at determining and comparing the conventional 87Sr/86Sr isotope ratios of a diverse set of Portland cements and their corresponding Portland clinkers, the major component of these cements. Two approaches to remove the additives from the cements, i.e. to measure the conventional 87Sr/86Sr isotopic fingerprint of the clinker only, were tested, namely, treatment with a potassium hydroxide/sucrose solution and sieving on a 11-µm sieve. Dissolution in concentrated hydrochloric acid/nitric acid and in diluted nitric acid was employed to determine the 87Sr/86Sr isotope ratios of the cements and the individual clinkers. The aim was to find the most appropriate sample preparation procedure for cement provenancing, and the selection was realised by comparing the 87Sr/86Sr isotope ratios of differently treated cements with those of the corresponding clinkers. None of the methods to separate the clinkers from the cements proved to be satisfactory. However, it was found that the 87Sr/86Sr isotope ratios of clinker and cement generally corresponded, meaning that the latter can be used as a proxy for the clinker 87Sr/86Sr isotope ratio. Finally, the concentrated hydrochloric acid/nitric acid dissolution method was found to be the most suitable sample preparation method for the cements; it is thus recommended for 87Sr/86Sr isotope analyses for cement provenancing. Graphical abstract

2021 ◽  
Vol 2081 (1) ◽  
pp. 012029
A V Shepelin ◽  
A M Rostom ◽  
V A Tomilin ◽  
L V Il’ichov

Abstract We propose a new model, entitled S-CTC, for description of quantum systems in the presence of CTC – closed time-like curves. The model is based on the viewpoint on any quantum state as an observer’s state of knowledge of the system preparation procedure. We compare and contrast our S-CTC model with D-CTC and P-CTC models and show that S-CTC shares special quantum features with both D-CTC and P-CTC. As far as the interaction of the quantum system with itself coming from the future concerns, S-CTC is formally equivalent to P-CTC. On the other hand, when calculating outcome probabilities for a measurement within the time interval between the entrance and exit of CTC, S-CTC becomes equivalent to D-CTC. Both these models require the concept of alternative realities (worlds) where different measurement outcomes are recorded and alternative connections of these realities by CTC.

mSystems ◽  
2021 ◽  
Christopher A. Gaulke ◽  
Emily R. Schmeltzer ◽  
Mark Dasenko ◽  
Brett M. Tyler ◽  
Rebecca Vega Thurber ◽  

Metagenomic library preparation methods and sequencing technologies continue to advance rapidly, allowing researchers to characterize microbial communities in previously underexplored environmental samples and systems. However, widely accepted standardized library preparation methods can be cost-prohibitive.

2021 ◽  
Vol 168 (9) ◽  
pp. 090531
Lina Qiu ◽  
Zhipeng Liu ◽  
Weiwei Zhang ◽  
Aijun Gong ◽  
Yang Liu

2021 ◽  
pp. e00253
Quang Huy Vu ◽  
Hy Triet Van ◽  
Van Thanh Tran ◽  
Nhat Nguyen Tran ◽  
Thi Diem Phuc Huynh ◽  

Genome ◽  
2021 ◽  
Hongjun Wang ◽  
Sen Xu

Extracting high molecular weight DNA is critical for successful long read DNA sequencing. Here we present a DNA preparation procedure combining CTAB DNA extraction protocol and a short read eliminator kit, which consistently produces high yields of high molecular weight DNA in the freshwater microcrustacean Daphnia. This method only requires common chemicals and equipment, providing researchers a viable alternative to DNA extraction kits that often require specialized tools.

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