scholarly journals Amperometric Biosensors Based on Direct Electron Transfer Enzymes

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4525
Author(s):  
Franziska Schachinger ◽  
Hucheng Chang ◽  
Stefan Scheiblbrandner ◽  
Roland Ludwig
Keyword(s):  
Electron Transfer ◽  
Electrode Materials ◽  
Direct Electron Transfer ◽  
Accurate Determination ◽  
Direct Electrochemistry ◽  
Product Analysis ◽  
Amperometric Biosensors ◽  
Chemical Surface ◽  
Fusion Enzymes ◽  
Cost Efficient

The accurate determination of analyte concentrations with selective, fast, and robust methods is the key for process control, product analysis, environmental compliance, and medical applications. Enzyme-based biosensors meet these requirements to a high degree and can be operated with simple, cost efficient, and easy to use devices. This review focuses on enzymes capable of direct electron transfer (DET) to electrodes and also the electrode materials which can enable or enhance the DET type bioelectrocatalysis. It presents amperometric biosensors for the quantification of important medical, technical, and environmental analytes and it carves out the requirements for enzymes and electrode materials in DET-based third generation biosensors. This review critically surveys enzymes and biosensors for which DET has been reported. Single- or multi-cofactor enzymes featuring copper centers, hemes, FAD, FMN, or PQQ as prosthetic groups as well as fusion enzymes are presented. Nanomaterials, nanostructured electrodes, chemical surface modifications, and protein immobilization strategies are reviewed for their ability to support direct electrochemistry of enzymes. The combination of both biosensor elements—enzymes and electrodes—is evaluated by comparison of substrate specificity, current density, sensitivity, and the range of detection.

A direct electron transfer biosensor based on a horseradish peroxidase and gold nanotriangle modified electrode and electrocatalysis

2018 ◽  
Vol 10 (44) ◽  
pp. 5297-5304 ◽  
Author(s):  
Yanyan Niu ◽  
Juan Liu ◽  
Wei Chen ◽  
Chunxiao Yin ◽  
Wenju Weng ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Ionic Liquid ◽  
Horseradish Peroxidase ◽  
Modified Electrode ◽  
Direct Electron Transfer ◽  
Direct Electrochemistry ◽  
Carbon Ionic Liquid Electrode ◽  
Liquid Electrode ◽  
Gold Nanotriangles

Gold nanotriangles (AuNTs) were used as a modifier on the surface of a carbon ionic liquid electrode (CILE), which was applied to realize direct electrochemistry and electrocatalysis of horseradish peroxidase (HRP).

scholarly journals Construction of myoglobin–amphiphilic alginate caprylamide–graphene composite modified electrode for the direct electron transfer between redox proteins and electrode and electrocatalysis of myoglobin

RSC Advances ◽  
2018 ◽  
Vol 8 (66) ◽  
pp. 38003-38012
Author(s):  
Xiuqiong Chen ◽  
Huiqiong Yan ◽  
Wei Sun ◽  
Guangying Chen ◽  
Changjiang Yu ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Modified Electrode ◽  
Trichloroacetic Acid ◽  
Direct Electron Transfer ◽  
Accurate Determination ◽  
Redox Proteins ◽  
Graphene Composite

To achieve the dispersion of the hydrophobic graphene (GR), the amphiphilic alginate caprylamide (ACA) was synthesized to fabricate electroactive Nafion/Mb–ACA–GR/CILE for the accurate determination of trichloroacetic acid (TCA).

Amperometric biosensors based on an apparent direct electron transfer between electrodes and immobilized peroxidases. Plenary lecture

The Analyst ◽  
1992 ◽  
Vol 117 (8) ◽  
pp. 1235-1241 ◽  
Author(s):  
Lo Gorton ◽  
Gunilla Jönsson-Pettersson ◽  
Elisabeth Csöregi ◽  
Kristina Johansson ◽  
Elena Domínguez ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Plenary Lecture ◽  
Amperometric Biosensors

scholarly journals Direct Electrochemical Enzyme Electron Transfer on Electrodes Modified by Self-Assembled Molecular Monolayers

Catalysts ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1458
Author(s):  
Xiaomei Yan ◽  
Jing Tang ◽  
David Tanner ◽  
Jens Ulstrup ◽  
Xinxin Xiao
Keyword(s):  
Electron Transfer ◽  
Electrode Materials ◽  
Direct Electron Transfer ◽  
Fine Tuning ◽  
Enzyme Adsorption ◽  
Redox Proteins ◽  
Characterization Methods ◽  
Molecular Monolayers ◽  
Wide Range ◽  
Self Assembled

Self-assembled molecular monolayers (SAMs) have long been recognized as crucial “bridges” between redox enzymes and solid electrode surfaces, on which the enzymes undergo direct electron transfer (DET)—for example, in enzymatic biofuel cells (EBFCs) and biosensors. SAMs possess a wide range of terminal groups that enable productive enzyme adsorption and fine-tuning in favorable orientations on the electrode. The tunneling distance and SAM chain length, and the contacting terminal SAM groups, are the most significant controlling factors in DET-type bioelectrocatalysis. In particular, SAM-modified nanostructured electrode materials have recently been extensively explored to improve the catalytic activity and stability of redox proteins immobilized on electrochemical surfaces. In this report, we present an overview of recent investigations of electrochemical enzyme DET processes on SAMs with a focus on single-crystal and nanoporous gold electrodes. Specifically, we consider the preparation and characterization methods of SAMs, as well as SAM applications in promoting interfacial electrochemical electron transfer of redox proteins and enzymes. The strategic selection of SAMs to accord with the properties of the core redox protein/enzymes is also highlighted.

A Direct Electrode-Driven Immobilized Catalase for Biocatalysis of Peroxides

2008 ◽  
Vol 6 (1) ◽  
Author(s):  
Huihong Liu ◽  
Xue-Ling Huang ◽  
Qi-Hui Wang
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Direct Electrochemistry ◽  
Cumene Hydroperoxide ◽  
Solution Ph ◽  
Electrochemical Behaviors ◽  
Aqueous Buffer ◽  
Agarose Hydrogel ◽  
Immobilized Catalase ◽  
Aqueous Mixture

The direct electrochemistry of catalase (Cat) immobilized in agarose hydrogel film on glassy carbon (GC) electrode was investigated. A pair of well-defined and quasi-reversible redox peaks both in aqueous buffer and ethanol-aqueous mixture was obtained. The formal potential (E°') indicates the reactions origin from the direct electron transfer between the CatFe(III)/(II) couple and the electrode surface. The E°' is linearly dependent on solution pH (redox Bohr effect), indicating the electron transfer of the CatFe(III)/(II) redox couple accompanied with the transfer of a proton. The electron transfer can be facilitated in ethanol-aqueous mixture for the reason of partial dehydration of the distal heme pocket. The water pools in agarose hydrogel play an important role in preventing either the change of conformation or the inactivity of Cat from polar organic solvent. Cat retains its original conformation in the agarose film that was confirmed by UV-Vis spectra and electrochemical behaviors in comparison to the controlled experiments using free hemin. The catalytic reactions to peroxides (hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, 2-butanone peroxide) using Cat-agarose modified electrode were also explored both in aqueous buffer and ethanol-aqueous mixture. The method could be application to the preparation of the hydrogel based third generation biosensors or bioreactors in organic media.

Sign in / Sign up

Export Citation Format

Share Document