scholarly journals Older Consumers’ Readiness to Accept Alternative, More Sustainable Protein Sources in the European Union

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1904 ◽  
Author(s):  
Alessandra C. Grasso ◽  
Yung Hung ◽  
Margreet R. Olthof ◽  
Wim Verbeke ◽  
Ingeborg A. Brouwer
Keyword(s):  
Older Adults ◽  
Single Cell ◽  
Protein Intake ◽  
Single Cell Protein ◽  
Protein Source ◽  
Cell Protein ◽  
Protein Sources ◽  
In Vitro Meat ◽  
Increase Protein Intake

Protein-energy malnutrition (PEM) is a growing concern on account of an aging population and its negative health consequences. While dietary protein plays a key role in the prevention of PEM, it also plays a pivotal role in the environmental impact of the human diet. In search for sustainable dietary strategies to increase protein intake in older adults, this study investigated the readiness of older adults to accept the consumption of the following alternative, more sustainable protein sources: plant-based protein, insects, single-cell protein, and in vitro meat. Using ordinal logistic regression modeling, the associations of different food-related attitudes and behavior and sociodemographics with older adults’ acceptance to consume such protein sources were assessed. Results were obtained through a consumer survey among 1825 community-dwelling older adults aged 65 years or above in five EU countries (United Kingdom, the Netherlands, Poland, Spain, and Finland). Dairy-based protein was generally the most accepted protein source in food products (75% of the respondents found its consumption acceptable or very acceptable). Plant-based protein was the most accepted alternative, more sustainable protein source (58%) followed by single-cell protein (20%), insect-based protein (9%), and in vitro meat-based protein (6%). We found that food fussiness is a barrier to acceptance, whereas green eating behavior and higher educational attainment are facilitators to older adults’ acceptance to eat protein from alternative, more sustainable sources. Health, sensory appeal, and price as food choice motives, as well as gender and country of residence were found to influence acceptance, although not consistently across all the protein sources. Findings suggest that there is a window of opportunity to increase older adults’ acceptance of alternative, more sustainable protein sources and in turn increase protein intake in an environmentally sustainable way in EU older adults.

Single cell protein: Sources, mechanism of production, nutritional value and its uses in aquaculture nutrition

Aquaculture ◽  
2021 ◽  
Vol 531 ◽  
pp. 735885 ◽  
Author(s):  
Muhammad Sharif ◽  
Muhammad Hammad Zafar ◽  
Amjad Islam Aqib ◽  
Muhammad Saeed ◽  
Mayada R. Farag ◽  
...  
Keyword(s):  
Single Cell ◽  
Nutritional Value ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Protein Sources

scholarly journals A transdisciplinary approach to the initial validation of a single cell protein as an alternative protein source for use in aquafeeds

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3170 ◽  
Author(s):  
Michael Tlusty ◽  
Andrew Rhyne ◽  
Joseph T. Szczebak ◽  
Bradford Bourque ◽  
Jennifer L. Bowen ◽  
...  
Keyword(s):  
Single Cell ◽  
Cost Effective ◽  
Taste Preference ◽  
Single Cell Protein ◽  
Protein Source ◽  
White Shrimp ◽  
Cell Protein ◽  
Methylobacterium Extorquens ◽  
Transdisciplinary Approach ◽  
Alternative Protein

The human population is growing and, globally, we must meet the challenge of increased protein needs required to feed this population. Single cell proteins (SCP), when coupled to aquaculture production, offer a means to ensure future protein needs can be met without direct competition with food for people. To demonstrate a given type of SCP has potential as a protein source for use in aquaculture feed, a number of steps need to be validated including demonstrating that the SCP is accepted by the species in question, leads to equivalent survival and growth, does not result in illness or other maladies, is palatable to the consumer, is cost effective to produce and can easily be incorporated into diets using existing technology. Here we examine white shrimp (Litopenaeus vannamei) growth and consumer taste preference, smallmouth grunt (Haemulon chrysargyreum) growth, survival, health and gut microbiota, and Atlantic salmon (Salmo salar) digestibility when fed diets that substitute the bacteriumMethylobacterium extorquensat a level of 30% (grunts), 100% (shrimp), or 55% (salmon) of the fishmeal in a compound feed.In each of these tests, animals performed equivalently when fed diets containingM. extorquensas when fed a standard aquaculture diet. This transdisciplinary approach is a first validation of this bacterium as a potential SCP protein substitute in aquafeeds. Given the ease to produce this SCP through an aerobic fermentation process, the broad applicability for use in aquaculture indicates the promise ofM. extorquensin leading toward greater food security in the future.

scholarly journals Potential of Bacillus cereus SN7 as a Single Cell Protein Source

2021 ◽  
Vol 2049 (1) ◽  
pp. 012024
Author(s):  
F Feliatra ◽  
M Mardalisa ◽  
P R Mukti ◽  
V A Feliatra ◽  
I Effendi
Keyword(s):  
Bacillus Cereus ◽  
Single Cell ◽  
Single Cell Protein ◽  
Growth Potential ◽  
Protein Source ◽  
Plate Count ◽  
Cell Protein ◽  
Growth Media ◽  
Carbohydrate Source ◽  
Average Growth

Abstract Single-cell protein (SCP) is a protein source produced from single-cell organisms, one of which is bacteria. Bacillus cereus SN7 is a potential isolate from a group of heterotrophic bacteria that has been isolated from the mouth of the Siak River, Riau Province, Indonesia. This study aims to analyze the potential of B. cereus SN7 bacteria using different growth media. The method used is an experimental method using Bacillus cereus SN7 bacterial isolate with different protein sources (eggs and skim milk) at different concentrations (8%, 10%, and 12%). All of treatments used the same carbohydrate source, Sago with 3 replications in each treatment. Measurement of bacterial culture growth was carried out every 6 hours, 12 hours, 18 hours and 24 hours based on the total plate count (TPC) method and bacterial cell biomass. The result show that the most optimal growth potential for Bacillus cereus SN7 bacteria is sago media added with 12% egg white as a protein source. This data have the same results as growth in commercial culture media (control). The growth of Bacillus cereus SN7 isolates in each treatment medium had almost the same growth pattern at the beginning, which experienced an exponential phase at 6 to 12 hours of incubation time. Meanwhile, in milk sago media, the average growth was not very good because the exponential and stationary phases were shorter.

scholarly journals Safety of Alternative Proteins: Technological, Environmental and Regulatory Aspects of Cultured Meat, Plant-Based Meat, Insect Protein and Single-Cell Protein

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1226
Author(s):  
Joshua Hadi ◽  
Gale Brightwell
Keyword(s):  
Food Safety ◽  
Single Cell ◽  
Environmental Impacts ◽  
Single Cell Protein ◽  
Regulatory Framework ◽  
Cell Protein ◽  
Protein Sources ◽  
Cultured Meat ◽  
Safety Aspects ◽  
Safety Risks

Food security and environmental issues have become global crises that need transformative solutions. As livestock production is becoming less sustainable, alternative sources of proteins are urgently required. These include cultured meat, plant-based meat, insect protein and single-cell protein. Here, we describe the food safety aspects of these novel protein sources, in terms of their technological backgrounds, environmental impacts and the necessary regulatory framework for future mass-scale production. Briefly, cultured meat grown in fetal bovine serum-based media can be exposed to viruses or infectious prion, in addition to other safety risks associated with the use of genetic engineering. Plant-based meat may contain allergens, anti-nutrients and thermally induced carcinogens. Microbiological risks and allergens are the primary concerns associated with insect protein. Single-cell protein sources are divided into microalgae, fungi and bacteria, all of which have specific food safety risks that include toxins, allergens and high ribonucleic acid (RNA) contents. The environmental impacts of these alternative proteins can mainly be attributed to the production of growth substrates or during cultivation. Legislations related to novel food or genetic modification are the relevant regulatory framework to ensure the safety of alternative proteins. Lastly, additional studies on the food safety aspects of alternative proteins are urgently needed for providing relevant food governing authorities with sufficient data to oversee that the technological progress in this area is balanced with robust safety standards.

scholarly journals Combined aptamer and transcriptome sequencing of single cells

2017 ◽  
Author(s):  
Cyrille L. Delley ◽  
Leqian Liu ◽  
Maen F. Sarhan ◽  
Adam R. Abate
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Expression Patterns ◽  
Cell Types ◽  
Single Cell Protein ◽  
Protein Characterization ◽  
Cell Protein ◽  
Surface Binding ◽  
Distinct Cell

AbstractThe transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single cell sequencing. With our method, we differentiate distinct cell types based on aptamer surface binding and gene expression patterns. Aptamers provide advantages over antibodies for single cell protein characterization, including rapid, in vitro, and high-purity generation via SELEX, and the ability to amplify and detect them with PCR and sequencing.

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