Altered Umbilical Cord Blood Nutrient Levels, Placental Cell Turnover and Transporter Expression in Human Term Pregnancies Conceived by Intracytoplasmic Sperm Injection (ICSI)

Assisted reproductive technologies (ART) may increase risk for abnormal placental development, preterm delivery and low birthweight. We investigated placental morphology, transporter expression and paired maternal/umbilical fasting blood nutrient levels in human term pregnancies conceived naturally (n = 10) or by intracytoplasmic sperm injection (ICSI; n = 11). Maternal and umbilical vein blood from singleton term (>37 weeks) C-section pregnancies were assessed for levels of free amino acids, glucose, free fatty acids (FFA), cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL) and triglycerides. We quantified placental expression of GLUT1 (glucose), SNAT2 (amino acids), P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) (drug) transporters, and placental morphology and pathology. Following ICSI, placental SNAT2 protein expression was downregulated and umbilical cord blood levels of citrulline were increased, while FFA levels were decreased at term (p < 0.05). Placental proliferation and apoptotic rates were increased in ICSI placentae (p < 0.05). No changes in maternal blood nutrient levels, placental GLUT1, P-gp and BCRP expression, or placental histopathology were observed. In term pregnancies, ICSI impairs placental SNAT2 transporter expression and cell turnover, and alters umbilical vein levels of specific nutrients without changing placental morphology. These may represent mechanisms through which ICSI impacts pregnancy outcomes and programs disease risk trajectories in offspring across the life course.

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Removal of 14 N-terminal amino acids of lactoferrin enhances its affinity for parenchymal liver cells and potentiates the inhibition of beta- very low density lipoprotein binding.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)74219-7 ◽

1993 ◽

Vol 268 (36) ◽

pp. 27069-27075

Author(s):

G J Ziere ◽

M K Bijsterbosch ◽

T J van Berkel

Keyword(s):

Amino Acids ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Liver Cells ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Parenchymal Liver ◽

Terminal Amino ◽

Lipoprotein Binding

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Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells: Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor

Metabolism ◽

10.1053/meta.2002.34703 ◽

2002 ◽

Vol 51 (9) ◽

pp. 1135-1142 ◽

Cited By ~ 30

Author(s):

Kazuo Sonoki ◽

Mototaka Yoshinari ◽

Masanori Iwase ◽

Kenzo Iino ◽

Kojiro Ichikawa ◽

...

Keyword(s):

Nitric Oxide ◽

Umbilical Vein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Nitric Oxide Donor ◽

Low Density ◽

Monocyte Chemoattractant Protein 1 ◽

Monocyte Chemoattractant ◽

Monocyte Chemoattractant Protein ◽

Umbilical Vein Endothelial Cells

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Delphinidin attenuates stress injury induced by oxidized low-density lipoprotein in human umbilical vein endothelial cells

Chemico-Biological Interactions ◽

10.1016/j.cbi.2009.09.024 ◽

2010 ◽

Vol 183 (1) ◽

pp. 105-112 ◽

Cited By ~ 30

Author(s):

Chun-ye Chen ◽

Long Yi ◽

Xin Jin ◽

Man-tian Mi ◽

Ting Zhang ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Oxidized Low Density Lipoprotein ◽

Stress Injury ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

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Rapamycin Promotes the Autophagic Degradation of Oxidized Low-Density Lipoprotein in Human Umbilical Vein Endothelial Cells

Journal of Vascular Research ◽

10.1159/000441143 ◽

2015 ◽

Vol 52 (3) ◽

pp. 210-219 ◽

Cited By ~ 9

Author(s):

Yanlin Zhang ◽

Qiao Han ◽

Shoujiang You ◽

Yongjun Cao ◽

Xia Zhang ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Oxidized Low Density Lipoprotein ◽

Human Umbilical Vein ◽

Autophagic Degradation ◽

Umbilical Vein Endothelial Cells

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Native low density lipoprotein increases the production of both nitric oxide and reactive oxygen species in the human umbilical vein endothelial cells

Genes & Genomics ◽

10.1007/s13258-018-00777-4 ◽

2019 ◽

Vol 41 (3) ◽

pp. 373-379 ◽

Cited By ~ 4

Author(s):

Hyun Joong Yoon ◽

Kee Oh Chay ◽

Sung Yeul Yang

Keyword(s):

Nitric Oxide ◽

Reactive Oxygen Species ◽

Endothelial Cells ◽

Umbilical Vein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Oxygen Species ◽

Umbilical Vein Endothelial Cells ◽

Native Low Density Lipoprotein

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Pioglitazone decreased CD40/CD40L expression on human umbilical vein endothelial cells induced by oxidized low-density lipoprotein

Clinica Chimica Acta ◽

10.1016/j.cca.2006.01.026 ◽

2006 ◽

Vol 370 (1-2) ◽

pp. 94-99 ◽

Cited By ~ 7

Author(s):

De-qian Jiang ◽

Luo-xiang Chu ◽

Zhao-yun Liu ◽

Shun-bao Zhang

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Oxidized Low Density Lipoprotein ◽

Human Umbilical Vein ◽

Cd40l Expression ◽

Umbilical Vein Endothelial Cells

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Th-P15:138 Effects of amino acids on the rate of oxidation of low density lipoprotein by iron at PH 4.5

Atherosclerosis Supplements ◽

10.1016/s1567-5688(06)82098-2 ◽

2006 ◽

Vol 7 (3) ◽

pp. 523

Author(s):

L. Satchell ◽

A. Islam ◽

M. Hamed ◽

J. Sharp ◽

I.S. McCue ◽

...

Keyword(s):

Amino Acids ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density

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Autophagy and Apoptosis in the Response of Human Vascular Endothelial Cells to Oxidized Low-Density Lipoprotein

Cardiology ◽

10.1159/000381332 ◽

2015 ◽

Vol 132 (1) ◽

pp. 27-33 ◽

Cited By ~ 10

Author(s):

Yanlin Zhang ◽

Ying Xie ◽

Shoujiang You ◽

Qiao Han ◽

Yongjun Cao ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

P53 Protein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Positive Staining ◽

Low Density ◽

Dependent Manner ◽

Oxidized Low Density Lipoprotein ◽

Concentration Dependent Manner

Objectives: Oxidized low-density lipoprotein (ox-LDL) may induce autophagy, apoptosis, necrosis or proliferation of cultured endothelial cells depending on the concentration and exposure time. Our previous studies found that ox-LDL exposure for 6 h increases the autophagic level of human umbilical vein endothelial cells (HUVECs) in a concentration-dependent manner. The present study investigates the relationship between autophagy and apoptosis in HUVECs exposed to ox-LDL. Methods: Flow cytometry and Western blot were used to study the apoptotic and autophagic phenomena. The contribution of autophagic and apoptotic mechanisms to ox-LDL-induced upregulation of MAP1-LC3, beclin1 and p53 protein levels were assessed by pretreatment with the autophagic inhibitors 3-MA and Atg5 small interfering (si)RNA, as well as z-vad-fmk, an apoptosis inhibitor. Results: ox-LDL induced the apoptosis of HUVECs in a concentration-dependent way. The increased expression of the autophagic proteins, LC3-II and beclin1, can be reversed by 3-MA and z-vad-fmk pretreatment. 3-MA and Atg5 siRNA increased the ox-LDL-induced increases of the p53 protein level and the annexin V-positive staining, which was decreased by z-vad-fmk. Conclusion: These results suggest that overstimulation of ox-LDL can induce autophagy and apoptosis in HUVECs. Inhibition of apoptosis leads to an inhibition of autophagy induced by ox-LDL. However, inhibition of autophagy leads to an increase in the ox-LDL-induced apoptosis.

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