scholarly journals Molecular Detection of Tick-Borne Pathogens in American Bison (Bison bison) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1428
Author(s):  
Diana M. Beristain-Ruiz ◽  
Cuauhcihuatl Vital-García ◽  
Julio V. Figueroa-Millán ◽  
José J. Lira-Amaya ◽  
Javier A. Garza-Hernández ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Biosphere Reserve ◽  
Bison Bison ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Babesia Bigemina ◽  
Emerging Pathogens ◽  
Wildlife Health ◽  
Rickettsia Rickettsii ◽  
Veterinary Importance ◽  
Pathogen Dna

American bison (Bison bison) is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were collected from 26 American bison in the JBR. We tested for the presence of Anaplasma marginale, Babesia bigemina, B. bovis, Borrelia burgdorferi sensu lato, and Rickettsia rickettsii DNA using nested and semi-nested PCR protocols performing duplicates in two different laboratories. Results showed three animals (11.5%) positive for B. burgdorferi s. l., three more (11.5%) for Rickettsia rickettsii, and four (19.2%) for B. bovis. Two individuals were co-infected with B. burgdorferi s. l. and B. bovis. We found no animals positive for A. marginale and B. bigemina. This is the first report in America of R. rickettsii in American bison. American bison has been described as an important reservoir for pathogens of zoonotic and veterinary importance; thus, the presence of tick-borne pathogen DNA in the JBR American bison indicates the importance of continuous wildlife health surveys.

Molecular detection of Rickettsia rickettsii in ticks associated with the bobcat (Lynx rufus) in northeast Mexico

2019 ◽  
Vol 10 (5) ◽  
pp. 1105-1108 ◽  
Author(s):  
Carmen Guzmán-Cornejo ◽  
Sokani Sánchez-Montes ◽  
Arturo Caso ◽  
Emilio Rendón-Franco ◽  
Claudia I. Muñoz-García
Keyword(s):  
Molecular Detection ◽  
Lynx Rufus ◽  
Rickettsia Rickettsii

Molecular Detection, Phylogenetic Analysis, and Genetic Diversity of Theileria annulata, Babesia bigemina, and Anaplasma marginale in Cattle in Three Districts of Egypt

2020 ◽  
Vol 65 (3) ◽  
pp. 620-627
Author(s):  
Khaled Mohamed El-Dakhly ◽  
Waleed M. Arafa ◽  
Saad Soliman ◽  
Omima Ramadan Abdel-Fatah ◽  
Ahmed Anwar Wahba ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Molecular Detection ◽  
Anaplasma Marginale ◽  
Theileria Annulata ◽  
Babesia Bigemina

Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents

2008 ◽  
Vol 53 (6) ◽  
pp. 493-499 ◽  
Author(s):  
A. Štefančíková ◽  
M. Derdáková ◽  
D. Lenčáková ◽  
R. Ivanová ◽  
M. Stanko ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Molecular Detection ◽  
Borrelia Burgdorferi Sensu Lato

Molecular Detection of Babesia bigemina in Subclinically Infected Cattle

2019 ◽  
Vol 21 (2) ◽  
pp. 117
Author(s):  
Munazah Shahzad ◽  
Rajat Garg ◽  
Anjali Devi ◽  
Shobha ◽  
Fayaz Ahmad Sheikh ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Infected Cattle ◽  
Babesia Bigemina

scholarly journals Molecular detection and characterization of Babesia bovis, Babesia bigemina, Theileria species and Anaplasma marginale isolated from cattle in Kenya

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Paul Franck Adjou Moumouni ◽  
Gabriel Oluga Aboge ◽  
Mohamad Alaa Terkawi ◽  
Tatsunori Masatani ◽  
Shinuo Cao ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Anaplasma Marginale ◽  
Babesia Bovis ◽  
Babesia Bigemina

scholarly journals Leptospira spp. and Toxoplasma gondii in stranded representatives of wild cetaceans in the Philippines

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Marie Christine M. Obusan ◽  
Ren Mark D. Villanueva ◽  
Maria Auxilia T. Siringan ◽  
Windell L. Rivera ◽  
Lemnuel V. Aragones
Keyword(s):  
Toxoplasma Gondii ◽  
Biological Samples ◽  
Molecular Detection ◽  
The Philippines ◽  
Biological Information ◽  
Emerging Pathogens ◽  
Cetacean Species ◽  
Serological Assay ◽  
Antibodies Detection

Abstract Background The stranding events of cetaceans in the Philippines provide opportunities for gathering biological information and specimens, especially from the pelagic forms. As part of an effort to monitor the health of wild cetaceans, this study detected Leptospira spp. and Toxoplasma gondii, causative agents of the emerging zoonotic diseases leptospirosis and toxoplasmosis respectively, in their stranded representatives. From October 2016–August 2018, 40 cetaceans (representing 14 species) that stranded nationwide were sampled for brain, cardiac muscle, skeletal muscle, kidney, and blood tissues, urine, and sera. These were subjected to molecular, serological, culture, and histopathological analyses to detect the target pathogens. Results T. gondii was detected in 20 (71%) of the 28 cetaceans with biological samples subjected to either molecular detection through RE gene amplification or IgG antibodies detection through agglutination-based serological assay. On the other hand, Leptospira was detected in 18 (64%) of 28 cetaceans with biological samples subjected to bacterial culture, molecular detection through 16S rDNA amplification, or IgM antibodies detection through ELISA-based serological assay. Conclusions There is the plausibility of toxoplasmosis and leptospirosis in cetacean populations found in the Philippines, however, acute or chronic phases of infections in sampled stranded individuals cannot be confirmed in the absence of supporting pathological observations and corroborating detection tests. Further studies should look for more evidences of pathogenicity, and explore the specific mechanisms by which pelagic cetacean species become infected by Leptospira spp. and T. gondii. As there is growing evidence on the role of cetaceans as sentinels of land-sea movement of emerging pathogens and the diseases they cause, any opportunity, such as their stranding events, should be maximized to investigate the health of their populations. Moreover, the role of leptospirosis or toxoplasmosis in these stranding events must be considered.

Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand

2012 ◽  
Vol 111 (3) ◽  
pp. 1259-1266 ◽  
Author(s):  
Shinuo Cao ◽  
Gabriel Oluga Aboge ◽  
Mohamad Alaa Terkawi ◽  
Longzheng Yu ◽  
Ketsarin Kamyingkird ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Babesia Bovis ◽  
Babesia Bigemina ◽  
Northern Thailand ◽  
Detection And Identification

scholarly journals Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato genospecies in red foxes (Vulpes vulpes) from Romania

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Mirabela Oana Dumitrache ◽  
Ioana Adriana Matei ◽  
Angela Monica Ionică ◽  
Zsuzsa Kalmár ◽  
Gianluca D’Amico ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Vulpes Vulpes ◽  
Molecular Detection ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Red Foxes

Detection of Borrelia burgdorferi sensu lato DNA in the blood of wild bison from Białowieza Primeval Forest in eastern Poland

2014 ◽  
Vol 17 (4) ◽  
pp. 713-715 ◽  
Author(s):  
Ł. Adaszek ◽  
B. Dzięgiel ◽  
M. Krzysiak ◽  
M. Skrzypczak ◽  
M. Adaszek ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Dna Amplification ◽  
Bison Bison ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Bison Bonasus ◽  
Primeval Forest ◽  
16S Rna ◽  
Pcr Products ◽  
Białowieża Primeval Forest ◽  
16S Rna Gene

Abstract The aim of the present study was to investigate the occurrence of Borrelia burgdorferi sensu lato DNA in a group of 120 wild bison (Bison bonasus) from the Bialowieza Primeval Forest in eastern Poland. The PCR technique revealed the presence of 16S RNA of Borrelia burgdorferi sensu lato in the blood of 16 out of 120 examined animals. DNA amplification by means of primers SC1 and SC2 gave a product with a size of 300-bp. The sequences of the PCR products obtained showed 100% homology with each other and 100% homology with B. burgdorferi s.l. 16S RNA gene DQ111061. Results of this study suggest that wild bison are important in maintaining agents of Lyme borreliosis, and that studies of reservoir competence of this species are indicated.

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