scholarly journals Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

2014 ◽  
Vol 39 (4) ◽  
pp. 649-653 ◽  
Author(s):  
S. A. Bhat ◽  
Harkirat Singh ◽  
N. K. Singh ◽  
S. S. Rath
Keyword(s):  
Molecular Detection ◽  
Babesia Bigemina ◽  
Central Plain ◽  
Healthy Cattle ◽  
Apparently Healthy

scholarly journals Prevalence and Molecular Detection of Quinolone-Resistant E. coli in Rectal Swab of Apparently Healthy Cattle in Bangladesh

2017 ◽  
Vol 24 (2) ◽  
pp. 1-7 ◽  
Author(s):  
Md-Montasir Mamun ◽  
Jayedul Hassan ◽  
K Nazir ◽  
Md-Alimul Islam ◽  
Khalada Zesmin ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Rectal Swab ◽  
E Coli ◽  
Healthy Cattle ◽  
Apparently Healthy

scholarly journals Phenotypic and Molecular Characterisation of Extended-Spectrum Beta-Lactamase ProducingEscherichia coliObtained from Animal Fecal Samples in Ado Ekiti, Nigeria

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Olugbenga Adekunle Olowe ◽  
Olufunmilayo Adewumi ◽  
Gbolabo Odewale ◽  
Olusola Ojurongbe ◽  
Olusolabomi Jose Adefioye
Keyword(s):  
Molecular Detection ◽  
Molecular Method ◽  
Fecal Samples ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Standard Procedures ◽  
Healthy Cattle ◽  
Third Generation Cephalosporins ◽  
Apparently Healthy

Production of extended-spectrumβ-lactamases (ESBLs) producingE. coliin animals and different methods of identifications from Ado Ekiti, Ekiti State, Nigeria, were investigated. Three hundred and fifty fecal samples, collected from apparently healthy cattle and pigs, were cultured and identified following standard procedures. ESBL phenotypic detection was carried out using combination disc test, double disc synergism test, and ESBL brilliance agar screening. Molecular detection of TEM, SHV, and CTX-M genes was carried out using standard molecular method. One hundred and fourteenE. coliisolates were recovered from the 350 samples processed, out of which 72 (63.2%) isolates were positive for ESBLs with multiple resistance to the antibiotics used. Eighty-one (71%) isolates were positive for ESBL by combination disc test, 90 (78.9%) were positive for double disc synergism test, and 93 (81.6%) were positive for ESBL brilliance agar. TEM and CTX-M genes were detected in 48 (42.1%) and 51 (44.7%) isolates, respectively. SHV gene was not detected in any of the isolates while TEM and CTX-M were detected in 33 (28.9%) isolates. This study showed high resistance ofE. colito antibiotics, particularly to the third generation cephalosporins. Regular monitoring and regulated use of antibiotics in livestock should be encouraged.

Molecular detection ofBabesiaspp. and other haemoparasitic infections of cattle in Maputo Province, Mozambique

Parasitology ◽  
2010 ◽  
Vol 137 (6) ◽  
pp. 939-946 ◽  
Author(s):  
TIAGO M. MARTINS ◽  
LUÍS NEVES ◽  
OLÍVIA C. PEDRO ◽  
JOSÉ M. FAFETINE ◽  
VIRGÍLIO E. DO ROSÁRIO ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
18S Rrna Gene ◽  
Babesia Bovis ◽  
Rrna Gene ◽  
Babesia Bigemina ◽  
Endemic Region ◽  
Phylogenic Tree ◽  
Hot Start ◽  
Healthy Cattle

SUMMARYMolecular detection ofBabesiaspecies in apparently healthy cattle within an endemic region was carried out in order to determine the prevalence of carriers and the geographical distribution ofBabesia bigeminaandBabesia bovisin Maputo Province, Mozambique. Samples from 477 animals at 5 localities were analysed using 2 techniques, the semi-nested hot-start PCR and the reverse line blot (RLB) assay. With the semi-nested hot-start PCR, detection ofB. bigeminaranged between 30% and 89%, and ofB. bovisbetween 27% and 83%. The RLB assay was comparatively less sensitive in this study and detection ofB. bovisranged from 0% to 17%, andB. bigeminawas not detected at all by this technique. Analysis of new sequences of the 18S rRNA gene revealed that the currentB. bigeminaRLB probe is not specific for the identification of isolates in Mozambique. The RLB assay, however, resulted in the detection of 8 other haemoparasite species belonging to the generaBabesia,Theileria,AnaplasmaandEhrlichia. 18S rRNA gene sequences from theTheileriaspp. were identified, and a phylogenic tree constructed with these sequences yielded a heterogeneousT. mutans-like group. In conclusion, infection withB. bigeminaandB. bovisis endemic in Maputo Province, but rates of transmission vary. Furthermore, mixed infections with the haemoparasites responsible for several tick-borne diseases in cattle are common in Mozambique.

scholarly journals Prevalence and molecular detection of fluoroquinolone-resistant genes (qnrA and qnrS) in Escherichia coli isolated from healthy broiler chickens

2018 ◽  
pp. 1720-1724 ◽  
Author(s):  
Shahin Mahmud ◽  
K. H. M. Nazmul Hussain Nazir ◽  
Md. Tanvir Rahman
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Molecular Detection ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
E Coli ◽  
Pcr Targeting ◽  
Apparently Healthy

Aim: The present study was carried out to determine the prevalence and molecular detection of fluoroquinolone-resistant Escherichia coli carrying qnrA and qnrS genes in healthy broiler chickens in Mymensingh, Bangladesh, and also to identify the genes responsible for such resistance. Materials and Methods: A total of 65 cloacal swabs were collected from apparently healthy chickens of 0-14 days (n=23) and 15-35 days (n=42) old. The samples were cultured onto Eosin Methylene Blue Agar, and the isolation and identification of the E. coli were performed based on morphology, cultural, staining, and biochemical properties followed by polymerase chain reaction (PCR) targeting E. coli 16S rRNA genes. The isolates were subjected to antimicrobial susceptibility test against five commonly used antibiotics under fluoroquinolone (quinolone) group, namely gatifloxacin, levofloxacin, moxifloxacin, ofloxacin, and pefloxacin by disk diffusion method. Detection of qnrA and qnrS genes was performed by PCR. Results: Among the 65 cloacal samples, 54 (83.08%) were found to be positive for E. coli. Antibiotic sensitivity test revealed that, of these 54 isolates, 18 (33.33%) were found to be resistant to at least one fluoroquinolone antibiotic. The highest resistance was observed against pefloxacin (61.11%). By PCR, of 18 E. coli resistant to fluoroquinolone, 13 (72.22%) were found to be positive for the presence of qnrS. None of the isolates were found positive for qnrA. Conclusion: Fluoroquinolone-resistant E. coli harboring qnrS genes is highly prevalent in apparently healthy broiler chickens and possesses a potential threat to human health.

scholarly journals Characterization of the major antigens of Haemophilus equigenitalis (contagious equine metritis organism)

1982 ◽  
Vol 89 (3) ◽  
pp. 529-538 ◽  
Author(s):  
M. J. Corbel ◽  
R. A. Brewer
Keyword(s):  
Molecular Weight ◽  
Cell Surface ◽  
High Molecular Weight ◽  
Protein Complex ◽  
Surface Antigens ◽  
Serological Response ◽  
Contagious Equine Metritis ◽  
Healthy Cattle ◽  
Apparently Healthy

SummaryImmunoelectrophoresis of ultrasonically disrupted Haemophilus equigenitalis (contagious equine metritis organism) cells against rabbit and equine antisera disclosed at least 11 precipitating antigens. Two of these, a polysaccharide and a lipopolysaccharide–protein complex, were of high molecular weight and located on the cell surface. The remaining antigens were intracellular and were small- to medium-sized proteins.The surface antigens were the most significant in relation to the serological response in infected horses. They also reacted with sera from apparently healthy cattle, but the reason for this was not determined. No serological cross-reaction between H. equigenitalis and species of Achromobacter and Moraxella was detected.

scholarly journals Prevalence and Serotype Diversity of Salmonella in Apparently Healthy Cattle: Systematic Review and Meta-Analysis of Published Studies, 2000–2017

2019 ◽  
Vol 6 ◽  
Author(s):  
Fanta D. Gutema ◽  
Getahun E. Agga ◽  
Reta D. Abdi ◽  
Lieven De Zutter ◽  
Luc Duchateau ◽  
...  
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Healthy Cattle ◽  
Apparently Healthy
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