scholarly journals Putative Role of a Yet Uncharacterized Protein Elicitor PeBb1 Derived from Beauveria bassiana ARSEF 2860 Strain against Myzus persicae (Homoptera: Aphididae) in Brassica rapa ssp. pekinensis

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 111 ◽  
Author(s):  
Talha Nazir ◽  
Abdul Hanan ◽  
Abdul Basit ◽  
Muhammad Zeeshan Majeed ◽  
Tauqir Anwar ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Brassica Rapa ◽  
Myzus Persicae ◽  
Genomic Sequence ◽  
Green Peach Aphid ◽  
Systemic Resistance ◽  
Uncharacterized Protein ◽  
Biological Pest Control ◽  
Protein Elicitor ◽  
Putative Role

This study reports the characterization of protein elicitor PeBb1 derived from entomopathogenic fungus Beauveria bassiana ARSEF-2860 strain and its putative role in induced systemic resistance in Brassica rapa ssp. pekinensis against green peach aphid Myzus persicae. The sequence of purified elicitor protein was matched with the genomic sequence of a hypothetical protein BBA_10269 from B. bassiana ARSEF-2860 (GenBank Accession No. XP_008603588.1). The protein-encoding gene PeBb1 contained 534 bp cDNA encoding a polypeptide of 177 amino acids with a molecular mass of 19 kDa. The recombinant elicitor protein was expressed in Escherichia coli using pET-28a (+) expression vector and induced necrosis in the leaves of tobacco. The effects of elicitor protein on aphid M. persicae was determined by applying three different concentrations of PeBb1 (i.e., 26, 35, 53 μM) on B. rapa plants at 4-leaf stage and the treated plants were exposed to newly emerged (0–6 h old) apterous adult aphids. Bioassay results showed significant (p < 0.05) sub-lethal effects of the exogenous application of PeBb1 elicitor on M. persicae. Moreover, the RT-qPCR gene expression analyses showed a significant up-regulation of most of the key genes linked to ethylene (ET)- and jasmonic acid (JA)-associated plant defense pathways in elicitor-treated plants. These results not only recommend the putative utilization of PeBb1 elicitor protein in future biological pest control strategies against phloem-feeding insect pests such as M. persicae, but also help in better comprehension of the mechanisms through which beneficial fungi trigger the induced plant resistance.

scholarly journals Interaction between the entomopathogenic fungus, Beauveria bassiana (Ascomycota: Hypocreales) and the parasitoid wasp, Aphidius colemani Viereck (Hymenoptera: Braconidae)

2013 ◽  
Vol 45 (1) ◽  
pp. 4 ◽  
Author(s):  
F. Emami ◽  
M. Alichi ◽  
K. Minaei
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Entomopathogenic Fungus ◽  
Green Peach Aphid ◽  
Parasitoid Wasp ◽  
Lethal Concentration ◽  
Aphidius Colemani ◽  
Parasitoid Development ◽  
Fungus Beauveria Bassiana ◽  
F1 Generation

The effect of the entomopathogenic fungus, <em>Beauveria bassiana</em>, on the biological characteristics of <em>Aphidius colemani</em>, a parasitoid of the green peach aphid, <em>Myzus persicae</em>, was studied under laboratory conditions. Third-instar nymphs of green peach aphid were infected with 5/3&times;105 conidia/mL of <em>B. bassiana</em>, which was determined to be the lethal concentration 50 dose. They were then offered to mated female parasitoids for 24 h at different intervals. Results showed that by prolonging the release intervals of parasitoids, the number of mummies and percent emergence of parasitoids were reduced. Moreover, production of male offspring increased in the F1 generation of parasitoids. The interference of <em>B. bassiana</em> with parasitoid development was also studied by first exposing the aphid hosts to the parasitoids for 24 h and subsequently spraying them with <em>B. bassiana</em> 24, 48, 72, and 96 h after exposure. Results showed that by prolonging fungal spraying intervals, the number of mummies and percent emergence of parasitoids were increased. It appeared that the best time for applying <em>B. bassiana</em> would be three to four days after parasitisation.

scholarly journals Pathogenicity of Beauveria bassiana isolates toward Leptinotarsa decemlineata [Coleoptera : Chrysomelidae], Myzus persicae [Homoptera : Aphididae] and their predator Coleomegilla maculata lengi [Coleoptera : Coccinellidae]

2005 ◽  
Vol 81 (1) ◽  
pp. 15-22 ◽  
Author(s):  
S.I. Todorova ◽  
D. Coderre ◽  
J.-C. Côté
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Leptinotarsa Decemlineata ◽  
Insect Pests ◽  
Green Peach Aphid ◽  
Coleomegilla Maculata ◽  
Potato Beetle ◽  
Potential Development ◽  
Beauveria Bassiana Isolates ◽  
Highly Virulent

Ten isolates of Beauveria bassiana from different sources and geographical sites were evaluated under laboratory conditions at a concentration of 107 conidia ml-1 for their pathogenicity against two insect pests, the Colorado potato beetle (Leptinotarsa decemlineata) and the green peach aphid (Myzus persicae), and their predator, the spotted ladybird beetle (Coleomegilla maculata lengi). Six isolates were highly virulent to all three insect species. Four others showed different degrees of specificity. The isolates 49, 233 and 210087 were the most interesting for their potential development as biological control agents because they were highly virulent for the two insect pests and caused low mortality in the coccinellid.

Compatibility of Insect Growth Regulators and Beauveria bassiana (Balsamo) Vuillemin in Controlling Green Peach Aphid (Homoptera: Aphididae) on Greenhouse Chrysanthemums

1999 ◽  
Vol 34 (3) ◽  
pp. 286-294 ◽  
Author(s):  
D. L. Olson ◽  
R. D. Oetting
Keyword(s):  
Beauveria Bassiana ◽  
Growth Regulators ◽  
Myzus Persicae ◽  
Laboratory Tests ◽  
Galleria Mellonella ◽  
Green Peach Aphid ◽  
Insect Growth Regulators ◽  
Insect Growth ◽  
Greater Wax Moth ◽  
Wax Moth

In laboratory and greenhouse studies, the compatibility of a spore suspension of Beauveria bassiana (Balsamo) with insect growth regulators (IGRs) was determined. When B. bassiana was applied over residues of 7 different IGRs, infectivity of greater wax moth larvae, Galleria mellonella (L), exposed to leaf rinsates was reduced by two of the IGRs. Azadirachtin and diflubenzuron reduced the infectivity of B. bassiana by up to 50%. Fenoxycarb, cyromazine, S-kinoprene, pyriproxyfen, and buprofezin did not significantly reduce the infectivity of B. bassiana. Under greenhouse conditions, the population levels of green peach aphid, Myzus persicae (Sulzer), on chrysanthemums, were not significantly different when Botanigard® (B. bassiana strain GHA) was used independently or in combination with the IGR S-kinoprene. In laboratory tests, 100% of wax moth larvae were infected with B. bassiana when they were exposed to leaf washes 24 h post Botanigard application to chrysanthemum foliage. Infectivity decreased rapidly with less than 15% of the larvae infected with B. bassiana when exposed to leaf washes 48 hr post Botanigard application to foliage.

Green peach aphid and potato leafroll virus: transmission and control

2020 ◽  
Author(s):  
R.A. Bagrov ◽  
◽  
V.I. Leunov
Keyword(s):  
Myzus Persicae ◽  
Green Peach Aphid ◽  
Virus Transmission ◽  
Potato Leafroll Virus ◽  
Factors Affecting ◽  
Potato Viruses ◽  
Tuber Necrosis ◽  
Aphid Vectors ◽  
Leafroll Virus ◽  
And Control

The mechanisms of transmission of potato viruses from plants to aphid vectors and from aphids to uninfected plants are described, including the example of the green peach aphid (Myzus persicae, GPA). Factors affecting the spreading of tuber necrosis and its manifestation on plants infected with potato leafroll virus (PLRV) are discussed. Recommendations for PLRV and GPA control in the field are given.

scholarly journals Insecticidal activity of citronella grass essential oil on Frankliniella schultzei and Myzus persicae

2013 ◽  
Vol 37 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Patrícia Fontes Pinheiro ◽  
Vagner Tebaldi de Queiroz ◽  
Vando Miossi Rondelli ◽  
Adilson Vidal Costa ◽  
Tiago de Paula Marcelino ◽  
...  
Keyword(s):  
Essential Oil ◽  
Insecticidal Activity ◽  
Myzus Persicae ◽  
Green Peach Aphid ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Economic Losses ◽  
Frankliniella Schultzei ◽  
Wide Range ◽  
Citronella Grass

The thrips, Frankliniella schultzei, and green peach aphid, Myzus persicae, cause direct damage to plants of economic importance and transmit phytoviruses, causing large economic losses. Chemical constituents of essential oils present a wide range of biological activities. The aim of this work was to evaluate insecticidal activity of essential oil from citronella grass, Cymbopogon winterianus, on F. schultzei and M. persicae. This essential oil was obtained by steam distillation and components were identified by GC/FID and GC/MS. A Potter spray tower was used to spray insects with the essential oil. The major constituents are geraniol (28.62%), citronellal (23.62%) and citronellol (17.10%). Essential oil of C. winterianus at 1% (w v-1) causes mortality in F. schultzei and M. persicae at 34.3% and 96.9%, respectively. The LC50 value for M. persicae was 0.36% and LC90 0.66%. Thus, citronella grass essential oil at 1% (w v-1) is more toxic to M. persicae than F. schultzei. This essential oil shows promise for developing pesticides to manage M. persicae.

STUDY REGARDING THE APHIDOFAGOUS FAUNA STRUCTURE IN THE PEACH ORCHARDS FROM CONSTANTA COUNTY IN 2020

2020 ◽  
Vol 13 ◽  
pp. 110-114
Author(s):  
Andrei Chiriloaie-Palade ◽  
Mădălina Radulea ◽  
Gheorghe Lămureanu ◽  
Ștefan Ion Mocanu ◽  
Maria Iamandei
Keyword(s):  
Myzus Persicae ◽  
Management System ◽  
Green Peach Aphid ◽  
Aphid Species ◽  
Insect Species ◽  
Essential Component ◽  
Intensive Use ◽  
Predatory Insect ◽  
Predatory Insects ◽  
Aphid Populations

"The cosmopolitan aphid species Myzus persicae is a key pest of peach orchards in south and southeastern Romania. The phenomenon of resistance induced by the intensive use of insecticides is a matter of concern for farmers and protectionists, making necessary integrated measure for the control of this pest. Conservation of natural enemy’s populations is an essential component of any management system proposed for pest aphids. The aim of the study was to determine the structure of predatory insects associated with Myzus persicae populations in peach orchards. The research was carried out in three orchards from two localities from Constanta County, in peach plantations with Springcrest variety aged 7, 11 and 12 years. As a result of this study, there were determined a total of 15 predatory insect species belonging to eight systematic families: Coccinellidae, Chrysopidae, Hemerobiidae, Syrphydae, Cecidomyiidae, Panorpidae, Nabidae and Forficulidae, which naturally contribute to the reduction of the green peach aphid populations. "

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