scholarly journals Pathogenicity of Beauveria bassiana isolates toward Leptinotarsa decemlineata [Coleoptera : Chrysomelidae], Myzus persicae [Homoptera : Aphididae] and their predator Coleomegilla maculata lengi [Coleoptera : Coccinellidae]

2005 ◽  
Vol 81 (1) ◽  
pp. 15-22 ◽  
Author(s):  
S.I. Todorova ◽  
D. Coderre ◽  
J.-C. Côté
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Leptinotarsa Decemlineata ◽  
Insect Pests ◽  
Green Peach Aphid ◽  
Coleomegilla Maculata ◽  
Potato Beetle ◽  
Potential Development ◽  
Beauveria Bassiana Isolates ◽  
Highly Virulent

Ten isolates of Beauveria bassiana from different sources and geographical sites were evaluated under laboratory conditions at a concentration of 107 conidia ml-1 for their pathogenicity against two insect pests, the Colorado potato beetle (Leptinotarsa decemlineata) and the green peach aphid (Myzus persicae), and their predator, the spotted ladybird beetle (Coleomegilla maculata lengi). Six isolates were highly virulent to all three insect species. Four others showed different degrees of specificity. The isolates 49, 233 and 210087 were the most interesting for their potential development as biological control agents because they were highly virulent for the two insect pests and caused low mortality in the coccinellid.

scholarly journals Virulence of Beauveria bassiana Strains Isolated from Cadavers of Colorado Potato Beetle, Leptinotarsa decemlineata

Insects ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1077
Author(s):  
Rostislav Zemek ◽  
Jana Konopická ◽  
Eva Jozová ◽  
Oxana Skoková Habuštová
Keyword(s):  
Beauveria Bassiana ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Reference Strain ◽  
The Czech Republic ◽  
Potato Beetle ◽  
Leptinotarsa Decemlineata Say ◽  
Native Strains ◽  
Chemical Pesticides ◽  
Fungus Beauveria Bassiana

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious, widely distributed pest of potato and other crops. This pest is able to defoliate the host plant and cause severe yield loss. Moreover, the pest quickly becomes resistant to many chemical pesticides. Therefore, the development of novel biopesticides targeting this pest is urgently needed. The purpose of this study was to obtain new strains of the entomopathogenic fungus Beauveria bassiana and assess their efficacy against L. decemlineata adults under laboratory conditions. Twelve strains were isolated from cadavers of Colorado potato beetles collected in potato fields in the Czech Republic. Test beetles were treated by suspensions of conidia at the concentration of 1 × 107 spores per milliliter and their survival was recorded daily for three weeks. The results of the bioassays revealed that all new native strains were pathogenic to L. decemlineata adults and caused mortality up to 100% at the end of the trial period with an LT50 of about 7 days. These strains were more virulent than a reference strain GHA and some of them can be recommended for the development of a new mycoinsecticide against L. decemlineata. Our findings also highlight the importance of searching for perspective strains of entomopathogenic fungi among naturally infected hosts.

scholarly journals Interaction between the entomopathogenic fungus, Beauveria bassiana (Ascomycota: Hypocreales) and the parasitoid wasp, Aphidius colemani Viereck (Hymenoptera: Braconidae)

2013 ◽  
Vol 45 (1) ◽  
pp. 4 ◽  
Author(s):  
F. Emami ◽  
M. Alichi ◽  
K. Minaei
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Entomopathogenic Fungus ◽  
Green Peach Aphid ◽  
Parasitoid Wasp ◽  
Lethal Concentration ◽  
Aphidius Colemani ◽  
Parasitoid Development ◽  
Fungus Beauveria Bassiana ◽  
F1 Generation

The effect of the entomopathogenic fungus, <em>Beauveria bassiana</em>, on the biological characteristics of <em>Aphidius colemani</em>, a parasitoid of the green peach aphid, <em>Myzus persicae</em>, was studied under laboratory conditions. Third-instar nymphs of green peach aphid were infected with 5/3&times;105 conidia/mL of <em>B. bassiana</em>, which was determined to be the lethal concentration 50 dose. They were then offered to mated female parasitoids for 24 h at different intervals. Results showed that by prolonging the release intervals of parasitoids, the number of mummies and percent emergence of parasitoids were reduced. Moreover, production of male offspring increased in the F1 generation of parasitoids. The interference of <em>B. bassiana</em> with parasitoid development was also studied by first exposing the aphid hosts to the parasitoids for 24 h and subsequently spraying them with <em>B. bassiana</em> 24, 48, 72, and 96 h after exposure. Results showed that by prolonging fungal spraying intervals, the number of mummies and percent emergence of parasitoids were increased. It appeared that the best time for applying <em>B. bassiana</em> would be three to four days after parasitisation.

scholarly journals POTENTIAL OF PROTEINASE INHIBITORS FOR THE PRODUCTION OF TRANSGENIC PLANTS TOLERANT TO INSECT PESTS. II. POTENTIAL LIMITATIONS

HortScience ◽  
1994 ◽  
Vol 29 (4) ◽  
pp. 249f-250
Author(s):  
Serge Overney ◽  
Dominique Michaud ◽  
Binh Nguyen-Quoc ◽  
Serge Yelle
Keyword(s):  
Leptinotarsa Decemlineata ◽  
Insect Pests ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Management Program ◽  
Potato Beetle ◽  
Perillus Bioculatus ◽  
Digestive Proteinases ◽  
Major Pest ◽  
Potential Risks

In recent years, several studies have demonstrated the potential of proteinase inhibitors (PIs) for controlling insect pests. Used as a component of an integrated pest management program, such an approach must, however, be considered with care, given the potential risks of interference on other control approaches. In particular, the effect of PIs on digestive proteinases of beneficial insects must be determined. As an example, this study analyzed the effect of oryzacystatins (OCs), two cysteine PIs isolated from rice, on digestive proteinases of Perillus bioculatus, a predator of the Colorado potato beetle (CPB; Leptinotarsa decemlineata Say), a major pest. Electrophoretic analyses showed the existence of several cysteine proteinase forms in the digestive tract of P. bioculatus. For each developmental stage, OCs dramatically inhibited proteolytic activity, showing an affinity between these inhibitors and the digestive proteinases of the predator. Despite their potential for controlling CPB, the two rice cystatins thus represent possible growth-suppressing compounds for the beneficial insect P. bioculatus. Work is currently under way to assess the compatibility of the two control approaches.

FOLIAR APPLICATIONS OF BEAUVERIA BASSIANA (BALSAMO) VUILLEMIN FOR CONTROL OF THE COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA (SAY) (COLEOPTERA: CHRYSOMELIDAE): AN OVERVIEW OF PILOT TEST RESULTS FROM THE NORTHERN UNITED STATES

1987 ◽  
Vol 119 (11) ◽  
pp. 959-974 ◽  
Author(s):  
Ann E. Hajek ◽  
Richard S. Soper ◽  
Donald W. Roberts ◽  
Thomas E. Anderson ◽  
K. Duane Biever ◽  
...  
Keyword(s):  
United States ◽  
Rhode Island ◽  
Beauveria Bassiana ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Primary Response ◽  
Control Plot ◽  
Pilot Test ◽  
Potato Beetle ◽  
Northern United States

AbstractAn overview of data from five research groups participating in a 3-year pilot test on the efficacy of foliar applications of Beauveria bassiana (Balsamo) Vuillemin for control of Colorado potato beetle, Leptinotarsa decemlineata (Say), populations in the northern United States is presented. Four treatments were used at each site: high and low B. bassiana dosages (5 × 1013 colony forming units (CFU) per hectare (ha) and 5 × 1012 CFU/ha), the recommended insecticide for each region, and an untreated check. The primary response variable was potato yield. For analyses considering test sites as replicates, during all 3 years the highest yields were produced in plots treated with insecticides. Within test sites, yields from plots receiving B. bassiana applications were greater than control plot yields in 8 of 24 trials but in only two of these instances, B. bassiana plot yields also did not differ from insecticide plot yields. Evaluating 1983 data from all states together, no differences were found between either high or low B. bassiana treatments and controls. The 1983 study design allowed no plot replication within states. In 1985, an altered plot design providing within-site replication demonstrated increased yield with increased dosage of B. bassiana when initial egg density was used as a covariate.Potato yields greater than control plot yields were noted with a minimum of 5.39 and 6.62 × 1011 CFU/ha B. bassiana. The lowest B. bassiana concentration producing yields not significantly different from insecticide plot yields was 6.62 × 1012 CFU/ha (Rhode Island, 1984). Defoliation ratings did not differ between B. bassiana treatments and controls in 1984 and 1985, although variability was found between yields.

scholarly journals Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)

Insects ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 35 ◽  
Author(s):  
Abdul Basit ◽  
Abdul Hanan ◽  
Talha Nazir ◽  
Muhammad Majeed ◽  
Dewen Qiu
Keyword(s):  
Phaseolus Vulgaris ◽  
Botrytis Cinerea ◽  
Myzus Persicae ◽  
Insect Pests ◽  
Green Peach Aphid ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Expression Levels ◽  
Temperature Regimes ◽  
Bean Plants

Elicitors are biofactors that induce resistance in plants against different insect pests. This in vitro study evaluated the impact of a novel elicitor protein PeBC1, extracted from a necrotrophic fungus Botrytis cinerea, on the development and fecundity parameters of green peach aphid (Myzus persicae) on common beans (Phaseolus vulgaris L.). Three different concentrations of PeBC1 elicitor (i.e., 33.56, 25.43, 19.33 µg mL−1) were applied at three different temperature regimes (i.e., 18, 21, and 25 °C). Elicitor treatments were applied topically on the bean plants at 3-leaf stage and newly emerged (0–6 h old) apterous adult aphids were exposed to these treated leaves. In addition to the biological parameters of aphids, the relative expression levels of key genes associated with jasmonic acid (JA) and salicylic acid (SA) plant defense pathways were also determined through RT-qPCR. Results of bioassays revealed that the application of PeBC1 elicitor protein exhibited pronounced and significant (p < 0.05) sub-lethal effects on green peach aphids. The fecundity was reduced and the nymphal development time was prolonged by different concentrations of PeBC1 elicitor and temperature regimes. Gene expression studies showed that the exogenous application of PeBC1 induced a significant upregulation of the expression levels of JA and SA pathway-associated genes in bean plants. As compared to control, elicitor-treated plants exhibited an induced resistance against aphids. Our findings suggest the potential use of PeBC1 elicitor protein in future bio-intensive management strategies against sap-sucking insect pests such as green peach aphids.

scholarly journals Pathogenicity of Beauveria bassiana strain 202 against sap-sucking insect pests

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 111-117 ◽  
Author(s):  
Bugti Ghulam Ali ◽  
Bin Wang ◽  
Na Cao ◽  
Feng Lin Hua
Keyword(s):  
Beauveria Bassiana ◽  
Bemisia Tabaci ◽  
Myzus Persicae ◽  
Insect Pests ◽  
Ornamental Plants ◽  
Fungal Strain ◽  
Insect Species ◽  
Experimental Trial ◽  
Fungal Inoculation

An experimental trial was conducted to determine the pathogenicity of Beauveria bassiana strain 202 (Bb-202) against multiple targeted sucking insect species that are serious pests of crops and ornamental plants. The insect species, Myzus persicae Sulzer (Hemiptera: Aphididae), Jacobiasca formosana Paoli (Hemiptera: Cicadellidae), Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), and Stephanitis nashi (Hemiptera: Tingidae) were exposed to conidia of B. bassiana at rates of 1.0 × 10<sup>2</sup>, 3.5 × 10<sup>3</sup>, 5 × 10<sup>4</sup>, and 6.75 × 10<sup>5 </sup>conidia/mm<sup>2</sup> to determine the pathogenicity of B. bassiana. The fungal strain Bb-202 showed the highest pathogenicity to M. persicae and 100% corrected mortality observed in the treatments over 1.0 × 10<sup>2 </sup>conidia/mm<sup>2</sup>, followed by the J. formosana with the final corrected mortality of 86.6, 94.4, and 97.4% after 10 days of fungal inoculation with 3.5 × 10<sup>3</sup>, 5 × 10<sup>4</sup>, and 6.75 × 10<sup>5 </sup>conidia/mm<sup>2</sup>, respectively. The strain Bb-202 also showed good pathogenicity to B. tabaci and corrected mortalities of 77.9 and 81.1% were recorded when exposed to 5 × 10<sup>4</sup> and 6.75 × 10<sup>5 </sup>conidia/mm<sup>2</sup>. Relatively weak pathogenicity was observed in S. nashi, in which the highest corrected mortality of 63.7% occurred at 6.75 × 10<sup>5 </sup>conidia/mm<sup>2</sup>. Accordingly, the LC<sub>50 </sub>and LT<sub>50 </sub>values of concentrations 1 × 10<sup>5</sup>, 1 × 10<sup>6</sup>, 1 × 10<sup>7</sup>, and 1 × 10<sup>8</sup> conidia/ml were calculated for M. persicae, J. formosana, B. tabaci, and S. nashi that were determined as 6.7 × 10<sup>4</sup>, 1.3 × 10<sup>6</sup>, 3.6 × 10<sup>6</sup>, and 1.2 × 10<sup>7</sup> conidia/ml and LT<sub>50</sub> was observed as 5.2~8.24, 5.1~6.6, 7.2~9.0, and 7.9~9.6 in days, respectively.

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