Anionic Long-Circulating Quantum Dots for Long-Term Intravital Vascular Imaging

A major impediment to the long-term in vivo vascular imaging is a lack of suitable probes and contrast agents. Our developed mercaptosuccinic acid (MSA) capped cadmium telluride/cadmium sulfide (CdTe/CdS) ultrasmall quantum dots (QDs) have high fluorescent quantum yield, long fluorescence lifetime and long half-life in blood, allowing high resolution long-term intravital vascular imaging. In this study, we showed that these QDs can be used to visualize the in vivo the vasculature in normal and cancerous livers in mice using multiphoton microscopy (MPM) coupled with fluorescence lifetime imaging (FLIM), with cellular resolution (~1 µm) up to 36 h after intravenous injection. Compared to highly regulated and controlled sinusoids in normal liver tissue, disordered, tortuous, and immature neovessels were observed in tumors. The utilized imaging methods have great potential as emerging tools in diagnosis and monitoring of treatment response in cancer.

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Multiphoton microscopy and fluorescence lifetime imaging provide a novel method in studying drug distribution and metabolism in the rat liver in vivo

Journal of Biomedical Optics ◽

10.1117/1.3614473 ◽

2011 ◽

Vol 16 (8) ◽

pp. 086013 ◽

Cited By ~ 15

Author(s):

Camilla A. Thorling ◽

Yuri Dancik ◽

Clinton W. Hupple ◽

Gregory Medley ◽

Xin Liu ◽

...

Keyword(s):

Rat Liver ◽

Fluorescence Lifetime ◽

Multiphoton Microscopy ◽

Drug Distribution ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging ◽

Novel Method

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Faculty Opinions recommendation of Combined non-linear laser imaging (two-photon excitation fluorescence microscopy, fluorescence lifetime imaging microscopy, multispectral multiphoton microscopy) in cutaneous tumours: first experiences.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1166974.629075 ◽

2009 ◽

Author(s):

Harvey Lui

Keyword(s):

Fluorescence Microscopy ◽

Fluorescence Lifetime ◽

Multiphoton Microscopy ◽

Fluorescence Lifetime Imaging Microscopy ◽

Fluorescence Lifetime Imaging ◽

Laser Imaging ◽

Two Photon ◽

Lifetime Imaging ◽

Photon Excitation ◽

Linear Laser

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In Vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots

Nano Letters ◽

10.1021/acs.nanolett.9b01708 ◽

2019 ◽

Vol 19 (8) ◽

pp. 5260-5265 ◽

Cited By ~ 14

Author(s):

Hongji Liu ◽

Xiangquan Deng ◽

Shen Tong ◽

Chen He ◽

Hui Cheng ◽

...

Keyword(s):

Quantum Dots ◽

Multiphoton Microscopy ◽

Deep Brain

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In Vivo Fluorescence Lifetime Imaging for Monitoring the Efficacy of the Cancer Treatment

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-13-1826 ◽

2014 ◽

Vol 20 (13) ◽

pp. 3531-3539 ◽

Cited By ~ 19

Author(s):

Yasaman Ardeshirpour ◽

Victor Chernomordik ◽

Moinuddin Hassan ◽

Rafal Zielinski ◽

Jacek Capala ◽

...

Keyword(s):

Cancer Treatment ◽

Fluorescence Lifetime ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging ◽

In Vivo Fluorescence

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Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway

International Journal of Molecular Sciences ◽

10.3390/ijms17040473 ◽

2016 ◽

Vol 17 (4) ◽

pp. 473 ◽

Cited By ~ 11

Author(s):

Leona Damalakiene ◽

Vitalijus Karabanovas ◽

Saulius Bagdonas ◽

Ricardas Rotomskis

Keyword(s):

Quantum Dots ◽

Fluorescence Lifetime ◽

Endocytic Pathway ◽

Fluorescence Lifetime Imaging Microscopy ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging

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Coarse-grained modeling of mitochondrial metabolism enables subcellular flux inference from fluorescence lifetime imaging microscopy

10.1101/2020.11.20.392225 ◽

2020 ◽

Author(s):

Xingbo Yang ◽

Daniel J. Needleman

Keyword(s):

Fluorescence Lifetime ◽

Metabolic Flux ◽

Living Cells ◽

Coarse Grained ◽

Fluorescence Lifetime Imaging Microscopy ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging ◽

Metabolic Fluxes ◽

Subcellular Resolution

AbstractMitochondria are central to metabolism and their dysfunctions are associated with many diseases1–9. Metabolic flux, the rate of turnover of molecules through a metabolic pathway, is one of the most important quantities in metabolism, but it remains a challenge to measure spatiotemporal variations in mitochondrial metabolic fluxes in living cells. Fluorescence lifetime imaging microscopy (FLIM) of NADH is a label-free technique that is widely used to characterize the metabolic state of mitochondria in vivo10–18. However, the utility of this technique has been limited by the inability to relate FLIM measurement to the underlying metabolic activities in mitochondria. Here we show that, if properly interpreted, FLIM of NADH can be used to quantitatively measure the flux through a major mitochondrial metabolic pathway, the electron transport chain (ETC), in vivo with subcellular resolution. This result is based on the use of a coarse-grained NADH redox model, which we test in mouse oocytes subject to a wide variety of perturbations by comparing predicted fluxes to direct biochemical measurements and by self-consistency criterion. Using this method, we discovered a subcellular spatial gradient of mitochondrial metabolic flux in mouse oocytes. We showed that this subcellular variation in mitochondrial flux correlates with a corresponding subcellular variation in mitochondrial membrane potential. The developed model, and the resulting procedure for analyzing FLIM of NADH, are valid under nearly all circumstances of biological interest. Thus, this approach is a general procedure to measure metabolic fluxes dynamically in living cells, with subcellular resolution.

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