scholarly journals Reply to “Comment on López-Yerena et al. ‘Absorption and Intestinal Metabolic Profile of Oleocanthal in Rats’ Pharmaceutics 2020, 12, 134”

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1221
Author(s):  
Anallely López-Yerena ◽  
Anna Vallverdú-Queralt ◽  
Raf Mols ◽  
Patrick Augustijns ◽  
Rosa M. Lamuela-Raventós ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Metabolic Profile ◽  
Scientific Community ◽  
Intestinal Perfusion ◽  
Perfusion Technique ◽  
Specific Data ◽  
Single Pass ◽  
Published Paper

Recently, in February 2020, we published a study exploring the intestinal absorption and metabolism of oleocanthal (OLC) in rats. A single-pass intestinal perfusion technique (SPIP) was used, involving simultaneous sampling from the luminal perfusate and mesenteric blood. Later, comments on our published paper were released, requesting clarification of specific data. In this detailed reply, we hope to have addressed and clarified all the concerns of A. Kaddoumi and K. El Sayed and that the scientific community will benefit from both the study and the comments it has generated.

Absorption Properties of Luteolin and Apigenin in Genkwa Flos Using In Situ Single-Pass Intestinal Perfusion System in the Rat

2017 ◽  
Vol 45 (08) ◽  
pp. 1745-1759 ◽  
Author(s):  
Xin He ◽  
Zi-Jing Song ◽  
Cui-Ping Jiang ◽  
Chun-Feng Zhang
Keyword(s):  
Small Intestine ◽  
Intestinal Absorption ◽  
Rat Model ◽  
Clinical Effectiveness ◽  
Intestinal Perfusion ◽  
Flower Bud ◽  
Transport Pathway ◽  
Absorption Enhancers ◽  
Single Pass

The flower bud of Daphne genkwa (Genkwa Flos) is a commonly used herbal medicine in Asian countries. Luteolin and apigenin are two recognized active flavonoids in Genkwa Flos. The aim of this study was to investigate the intestinal absorption mechanisms of Genkwa Flos flavonoids using in situ single-pass intestinal perfusion rat model. Using HPLC, we determined its major effective flavonoids luteolin, apigenin, as well as, hydroxygenkwanin and genkwanin in biological samples. The intestinal absorption mechanisms of the total flavonoids in Genkwa Flos (TFG) were investigated using in situ single-pass intestinal perfusion rat model. Comparing the TFG absorption rate in different intestinal segments, data showed that the small intestine absorption was significantly higher than that of the colon ([Formula: see text]). Compared with duodenum and ileum, the jejunum was the best small intestinal site for TFG absorption. The high TFG concentration (61.48[Formula: see text][Formula: see text]g/ml) yielded the highest permeability ([Formula: see text]). Subsequently, three membrane protein inhibitors (verapamil, pantoprazole and probenecid) were used to explore the TFG absorption pathways. Data showed probenecid, a multidrug resistance protein (or MRP) inhibitor, effectively enhanced the TFG absorption ([Formula: see text]). Furthermore, by comparing commonly used natural absorption enhancers on TFG, it was observed that camphor was the most effective. In Situ single-pass intestinal perfusion experiment shows that TFG absorption is much higher in the small intestine than in the colon, and the TFG is absorbed mainly via an active transport pathway with MRP-mediated efflux mechanism. Camphor obviously enhanced the TFG absorption, and this could be an effective TFG formulation preparation method to increase clinical effectiveness after Genkwa Flos administration. Our study elucidated the TFG absorption mechanisms, and provided new information for its formulation preparation.

Intestinal absorption mechanisms of araloside A in situ single-pass intestinal perfusion and in vitro Caco-2 cell model

2018 ◽  
Vol 106 ◽  
pp. 1563-1569 ◽  
Author(s):  
Hui Yang ◽  
Bingtao Zhai ◽  
Yu Fan ◽  
Jing Wang ◽  
Jing Sun ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Cell Model ◽  
Intestinal Perfusion ◽  
Single Pass

scholarly journals Intestinal absorption, metabolism, and excretion of (–)-epicatechin in healthy humans assessed by using an intestinal perfusion technique

2013 ◽  
Vol 98 (4) ◽  
pp. 924-933 ◽  
Author(s):  
Lucas Actis-Goretta ◽  
Antoine Lévèques ◽  
Maarit Rein ◽  
Alexander Teml ◽  
Christian Schäfer ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Intestinal Perfusion ◽  
Perfusion Technique ◽  
Healthy Humans

Intestinal Absorption of Cystine and Cysteine in Normal Human Subjects and Patients with Cystinuria

1974 ◽  
Vol 47 (4) ◽  
pp. 393-397 ◽  
Author(s):  
D. B. A. Silk ◽  
D. Perrett ◽  
A. D. Stephens ◽  
M. L. Clark ◽  
E. F. Scowen
Keyword(s):  
Intestinal Absorption ◽  
Human Subjects ◽  
Transport Processes ◽  
Intestinal Perfusion ◽  
Perfusion Technique ◽  
Normal Human

1. An intestinal perfusion technique has been used to study absorption in vivo of l-cystine and l-cysteine in six normal human subjects and three patients with homozygous cystinuria. 2. No significant absorption of l-cystine was detected during perfusion of the cystinuric patients, whereas l-cysteine absorption was normal. These results imply that l-cystine and l-cysteine are normally absorbed by different transport processes. 3. No significant reduction of l-cystine to l-cysteine occurred in the gut lumen during the perfusion experiments. No more oxidation of l-cysteine to l-cystine occurred in the gut lumen during the perfusion experiments than in the test solutions which were simultaneously incubated in vitro.

scholarly journals Intestinal absorption of forsythoside A in in situ single-pass intestinal perfusion and in vitro Caco-2 cell models

2012 ◽  
Vol 33 (8) ◽  
pp. 1069-1079 ◽  
Author(s):  
Wei Zhou ◽  
Liu-qing Di ◽  
Juan Wang ◽  
Jin-jun Shan ◽  
Shi-jia Liu ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Intestinal Perfusion ◽  
Cell Models ◽  
Single Pass
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