scholarly journals Corn Responsiveness to Azospirillum: Accessing the Effect of Root Exudates on the Bacterial Growth and Its Ability to Fix Nitrogen

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 923 ◽  
Author(s):  
Lucas Caiubi Pereira ◽  
Carolina Bertuzzi Pereira ◽  
Larissa Vinis Correia ◽  
Thaisa Cavalieri Matera ◽  
Rayssa Fernanda dos Santos ◽  
...  
Keyword(s):  
Energy Source ◽  
Organic Acids ◽  
Root Exudates ◽  
High Performance ◽  
Nitrogenase Activity ◽  
Genotypic Variation ◽  
Dry Mass ◽  
Corn Hybrids ◽  
Bacteria Growth ◽  
Root Tissues

Corn has shown different degrees of positive response to inoculation with the nitrogen- fixing bacteria of the genera Azospirillum. Part of it has been attributed to the plant genotypic variation, including the root exudates, that are used by the bacteria as energy source. In this study, we grew two corn hybrids that differ for their response to Azospirillum, to investigate the effect of different exudates profiles on the bacteria growth and nitrogenase activity. Employing high performance liquid chromatography, we identified nine amino acids (asparagine, aspartic acid, serine, glutamic acid, valine, phenylalanine, threonine, tryptophan and alanine), six sugars (glucose, sucrose, xylose, arabinose, fructose and galactose) and four organic acids (citrate, malate, succinate and fumarate). The less responsive corn genotype showed reduced plant growth (root volume, shoot dry mass and shoot N content), a lower concentration of Azospirillum cells within the root tissues, a higher content of asparagine and glucose and a reduced amount of metabolites that serve as bacterial energy source (all organic acids + five sugars, excluding glucose). The genotypes did not interfere in the ability of Azospirillum to colonize the substrate, but the metabolites released by the less responsive one reduced the nitrogenase activity.

scholarly journals Characteristics of Organic Acid Secretion Associated with the Interaction betweenBurkholderia multivoransWS-FJ9 and Poplar Root System

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Guan-Xi Li ◽  
Xiao-Qin Wu ◽  
Jian-Ren Ye ◽  
He-Chuan Yang
Keyword(s):  
Organic Acids ◽  
Root System ◽  
Root Exudates ◽  
High Performance ◽  
Interactive Effects ◽  
Available Phosphorus ◽  
Burkholderia Multivorans ◽  
Phosphorus Stress ◽  
Phosphate Solubilizing ◽  
Poplar Root

The objective of this study was to investigate whether plant-bacteria interaction affects the secretion of organic acids by both organisms and to assess whether the production of IAA by the bacterium increases the secretion of organic acids by root exudates, and if the stress produced by low available phosphorus (P) affects the production of organic acids by bacteria, by roots, or by root exudates in presence of bacterial cultures. With this purpose, we used as a biological model poplar plants and one strain ofBurkholderia multivoransable to solubilize P. High performance liquid chromatography was utilized to measure organic acids. The tests, the inductive effects of exogenous indole-3-acetic acid (IAA) on secretion of organic acids, the 2 × 4 × 2 factorial design experiment, and the ability of organic acids to solubilize tricalcium phosphate were performed to investigate the interactive effects. The results showed that, afterB. multivoransWS-FJ9 interacted with the poplar root system, the key phosphate-solubilizing driving force was gluconic acid (GA) which was produced in three ways: (1) secreted by the root system in the presence of IAA produced byB. multivoransWS-FJ9; (2) secreted byB. multivoransWS-FJ9; and (3) secreted by the poplar root system in the presence of phosphorus stress. When phosphorus stress was absent, the GA was produced as outlined in (1) and (2) above. These results demonstrated that inoculatingB. multivoransWS-FJ9 into the poplar root system could increase the amount of GA secretion and implied that the interaction betweenB. multivoransWS-FJ9 and the poplar root system could contribute to the increase of P available fraction for poplar plants.

Determination of organic acids in grape musts, wines and vinegars by high-performance liquid chromatography

1993 ◽  
Vol 655 (1) ◽  
pp. 111-117 ◽  
Author(s):  
E. García Romero ◽  
G. Sánchez Muñoz ◽  
P.J. Martín Alvarez ◽  
M.D. Cabezudo Ibáñez
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Organic Acids ◽  
High Performance ◽  
Grape Musts

scholarly journals Organic compounds in root exudates of Miscanthus × Giganteus greef et deu and limitation of microorganisms in its rhizosphere by nutrients

2010 ◽  
Vol 58 (5) ◽  
pp. 203-208 ◽  
Author(s):  
Hana Kaňová ◽  
Joffrey Carre ◽  
Valerie Vranová ◽  
Klement Rejšek ◽  
Pavel Formánek
Keyword(s):  
Organic Acids ◽  
Organic Compounds ◽  
Respiration Rate ◽  
Root Exudates ◽  
Plant Nutrients ◽  
Rhizospheric Soil ◽  
Polluted Soils ◽  
Rhizosphere Microorganisms ◽  
Miscanthus Giganteus

This study was conducted to determine the composition of sugars and organic acids in root exudates of Miscanthus × Giganteus and to find out if microorganisms of the rhizospheric soil are limited by mi­ne­ral nutrients. The following sugars and organic acids were determined in root exudates of this plant: glucose, saccharose, and acids such as succinic, propionic, citric, tartaric, malic, oxalic, ascorbic, acetic and fumaric. Respiration of soil from rhizosphere of Miscanthus × Giganteus was found to be limited by N, K and Ca. Respiration rate after application of mineral compounds increased in following orther: nitrate > calcium > potassium > ammonium, giving approx. 165, 99, 52 and 31 % increase compared to control. Further research is necessary to determine the role of plant nutrients from the point of their limitations for rhizosphere microorganisms, to broader very rare knowledges in this topic, especially for polluted soils to stimulate efficiency of phytoremediations.

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