scholarly journals Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1349
Author(s):  
Isha Mishra ◽  
Tahmish Fatima ◽  
Dilfuza Egamberdieva ◽  
Naveen Kumar Arora
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Brassica Juncea ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Parameter ◽  
Plant Growth Promoting Rhizobacteria ◽  
Threshold Level ◽  
Plant Growth Promoting ◽  
Growth Promoting

In this study, Pseudomonas putida BSP9 isolated from rhizosphere of Brassica juncea was investigated for its plant growth promoting and biosurfactant producing activities. The isolate showed the ability to produce indole acetic acid, siderophore, phosphate solubilization activity and was an efficient producer of biosurfactant. Purification (of the biosurfactant) by thin layer chromatography (TLC) and further characterization by Fourier transform infrared spectroscopy (FTIR) revealed that biosurfactant produced by the isolate belonged to the glycolipid category, which is largely produced by Pseudomonas sp. In addition, liquid chromatography-mass spectroscopy (LC-MS) analysis showed the presence of a mixture of six mono-rhamnolipidic and a di-rhamnolipidic congeners, confirming it as a rhamnolipid biosurfactant. Bioformulations were developed using BSP9 and its biosurfactant to check their impact on promoting plant growth in B. juncea. It was noted from the study that bioformulations amended with biosurfactant (singly or in combination with BSP9) resulted in enhancement in the growth parameters of B. juncea as compared to untreated control. Maximum increment was achieved by plants inoculated with bioformulation that had BSP9 plus biosurfactant. The study also suggested that growth promotion was significant up to a threshold level of biosurfactant and that further increasing the concentration did not further enhance the growth parameter values of the plant. The study proves that novel bioformulations can be developed by integrating plant growth promoting rhizobacteria (PGPR) and their biosurfactant, and they can be effectively used for increasing agricultural productivity while minimizing our dependence on agrochemicals.

scholarly journals Plant Growth Promoting Rhizobacteria, Arbuscular Mycorrhizal Fungi and Their Synergistic Interactions to Counteract the Negative Effects of Saline Soil on Agriculture: Key Macromolecules and Mechanisms

2021 ◽  
Vol 9 (7) ◽  
pp. 1491
Author(s):  
Alka Sagar ◽  
Parikshita Rathore ◽  
Pramod W. Ramteke ◽  
Wusirika Ramakrishna ◽  
Munagala S. Reddy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Acc Deaminase ◽  
Arbuscular Mycorrhizal ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deaminase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR and AMF enhances nutrient uptake and productivity of several crops compared to a single inoculation in both normal and stressed environments. Positively interacting PGPR + AMF combination is an efficient and cost-effective recipe for improving plant tolerance against salinity stress, which can be an extremely useful approach for sustainable agriculture.

scholarly journals Evaluation of two Pseudomonas strains isolated from a maize rhizosphere as a plant growth promoting rhizobacteria

2019 ◽  
Vol 2 (2) ◽  
pp. 38-46
Author(s):  
Thanh Nguyen Chu ◽  
Nhi Yen Nguyen ◽  
Diep Ngoc Dao ◽  
Bao Thi Hoai Tran ◽  
Minh Thi Thanh Hoang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
In Vitro Tests ◽  
Maize Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR) are free-living soil bacteria (rhizosphere bacteria), rhizoplane bacteria or endophytic bacteria that may promote plant growth and suppress plant diseases. The aim of this study is to evaluate effects of 2 rhizobacteria strains belonging to the genus Pseudomonas isolated from maize rhizosphere on the plant growth promotion. The in vitro tests showed that both of strain could fix nitrogen, solubilize phosphate, produced phytohormones (IAA and GAs), and improved the germination and growth of Arabidopsis thaliana. Under the greenhouse condition, growth parameters of bacteria inoculated maizes (fresh weight of shoot, dry weight of root, chlorophyll content) were also increased significantly than those of uninoculated ones. Our results reported 2 promising bacteria strain candidates and revealed their potential as a biological agent for eco-friendly agricultural practices.

Silicon and plant growth promoting rhizobacteria differentially regulate AgNP-induced toxicity in Brassica juncea: Implication of nitric oxide

2020 ◽  
Vol 390 ◽  
pp. 121806 ◽  
Author(s):  
Kanchan Vishwakarma ◽  
Vijay Pratap Singh ◽  
Sheo Mohan Prasad ◽  
Devendra Kumar Chauhan ◽  
Durgesh Kumar Tripathi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Plant Growth ◽  
Brassica Juncea ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Rhizobium leguminosarum as a plant growth-promoting rhizobacterium: direct growth promotion of canola and lettuce

1996 ◽  
Vol 42 (3) ◽  
pp. 279-283 ◽  
Author(s):  
T. C. Noel ◽  
C. Sheng ◽  
C. K. Yost ◽  
R. P. Pharis ◽  
M. F. Hynes
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Direct Growth ◽  
Early Seedling ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Early seedling root growth of the nonlegumes canola (Brassica campestris cv. Tobin, Brassica napus cv. Westar) and lettuce (Lactuca saliva cv. Grand Rapids) was significantly promoted by inoculation of seeds with certain strains of Rhizobium leguminosarum, including nitrogen- and nonnitrogen-fixing derivatives under gnotobiotic conditions. The growfh-promotive effect appears to be direct, with possible involvement of the plant growth regulators indole-3-acetic acid and cytokinin. Auxotrophic Rhizobium mutants requiring tryptophan or adenosine (precursors for indole-3-acetic acid and cytokinin synthesis, respectively) did not promote growth to the extent of the parent strain. The findings of this study demonstrate a new facet of the Rhizobium–plant relationship and that Rhizobium leguminosarum can be considered a plant growth-promoting rhizobacterium (PGPR).Key words: Rhizobium, plant growth-promoting rhizobacteria, PGPR, indole-3-acetic acid, cytokinin, roots, auxotrophic mutants.

Colonization and growth promotion of outplanted spruce seedlings pre-inoculated with plant growth-promoting rhizobacteria in the greenhouse

2000 ◽  
Vol 30 (6) ◽  
pp. 845-854 ◽  
Author(s):  
Masahiro Shishido ◽  
Christopher P Chanway
Keyword(s):  
Plant Growth ◽  
Seedling Growth ◽  
Picea Glauca ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Population Declines ◽  
Growth Responses ◽  
Relative Growth Rates ◽  
Plant Growth Promoting ◽  
Growth Promoting

Seeds of two hybrid spruce (Picea glauca (Moench) Voss × Picea engelmannii Parry ex Engelm.) ecotypes were inoculated with one of six plant growth-promoting rhizobacteria (PGPR) strains previously shown to be able to stimulate spruce growth in controlled environments. The resulting seedlings were grown in the greenhouse for 17 weeks before outplanting at four reforestation sites. Inoculation with five of the six strains caused significant seedling growth promotion in the greenhouse, which necessitated analysis of relative growth rates (RGR) to evaluate seedling performance in the field. Four months after outplanting, most strains enhanced spruce shoot or root RGRs in the field, but seedling growth responses were strain specific. For example, Pseudomonas strain Ss2-RN significantly increased both shoot and root RGRs by 10-234% at all sites, but increases of 28-70% were most common. In contrast, Bacillus strain S20-R was ineffective at all outplanting sites. In addition, seedlings inoculated with four of the six strains had significantly less shoot injury than control seedlings at all sites. Evaluation of root colonization by PGPR indicated that bacterial population declines were not related to spruce growth response variability in the field. Our results indicate that once plant growth promotion is induced in the greenhouse, seedling RGR can increase by more than 100% during the first growing season in the field. However RGR increases of 21-47% were more common and may be more representative of the magnitude of biomass increases that can result from PGPR inoculation.

Low temperature growth, freezing survival, and production of antifreeze protein by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2

1995 ◽  
Vol 41 (9) ◽  
pp. 776-784 ◽  
Author(s):  
Xiuying Sun ◽  
Marilyn Griffith ◽  
J. J. Pasternak ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Medium ◽  
Polyacrylamide Gel Electrophoresis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Antifreeze Protein ◽  
High Arctic ◽  
Major Protein ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth promoting rhizobacterium Pseudomonas putida GR12-2 was originally isolated from the rhizosphere of plants growing in the Canadian High Arctic. Here we report that this bacterium was able to grow and promote root elongation of both spring and winter canola at 5 °C, a temperature at which only a relatively small number of bacteria are able to proliferate and function. In addition, the bacterium survived exposure to freezing temperatures, i.e., −20 and −50 °C. In an effort to determine the mechanistic basis for this behaviour, it was discovered that following growth at 5 °C, P. putida GR12-2 synthesized and secreted to the growth medium a protein with antifreeze activity. Analysis of the spent growth medium, following concentration by ultrafiltration, by SDS-polyacrylamide gel electrophoresis revealed the presence of one major protein with a molecular mass of approximately 32–34 kDa and a number of minor proteins. However, at this point it is not known which of these proteins contains the antifreeze activity.Key words: plant growth promoting rhizobacteria, PGPR, bacterial fertilizer, soil bacteria, antifreeze protein.

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