scholarly journals Bioflocculants Produced by Bacterial Strains Isolated from Palm Oil Mill Effluent for Application in the Removal of Eriochrome Black T Dye from Water

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1545
Author(s):  
Syed Zaghum Abbas ◽  
Yang-Chun Yong ◽  
Moonis Ali Khan ◽  
Masoom Raza Siddiqui ◽  
Afnan Ali Hussain Hakami ◽  
...  

Four strains of bioflocculant-producing bacteria were isolated from a palm oil mill effluent (POME). The four bacterial strains were identified as Pseudomonas alcaliphila (B1), Pseudomonas oleovorans (B2), Pseudomonas chengduensis (B3), and Bacillus nitratireducens (B4) by molecular identification. Among the four bacterial strains, Bacillus nitratireducens (B4) achieved the highest flocculating activity (49.15%) towards kaolin clay suspension after eight hours of cultivation time and was selected for further studies. The optimum conditions for Eriochrome Black T (EBT) flocculation regarding initial pH, type of cation, and B4 dosage were determined to be pH 2, Ca2⁺ cations, and a dosage of 250 mL/L of nutrient broth containing B4. Under these conditions, above 90% of EBT dye removal was attained. Fourier transform infrared spectroscopic (FT-IR) analysis of the bioflocculant revealed the presence of hydroxyl, alkyl, carboxyl, and amino groups. This bioflocculant was demonstrated to possess a good flocculating activity, being a promissory, low-cost, harmless, and environmentally friendly alternative for the treatment of effluents contaminated with dyes.

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 601 ◽  
Author(s):  
Tawfiq J. H. Banch ◽  
Marlia M. Hanafiah ◽  
Salem S. Abu Amr ◽  
Abbas F. M. Alkarkhi ◽  
Mohammed Hasan

Sanitary landfilling is the most common method of removing urban solid waste in developing countries. Landfills contain high levels of organic materials, ammonia, and heavy metals, thereby producing leachate which causes a possible future pollution of ground and surface water. Recently, agricultural waste was considered a co-substratum to promote the biodegradation of organics in industrial wastewater. The use of low-cost and natural materials for wastewater treatment is now being considered by many researchers. In this study, palm oil mill effluent (POME) was used for treating stabilized leachate from old landfill. A set of preliminary experiments using different POME/leachate ratios and aeration times was performed to identify the setting of experimental design and optimize the effect of employing POME on four responses: chemical oxygen demand (COD), total suspended solids (TSS), color, and ammoniacal nitrogen (NH3-N). The treatment efficiency was evaluated based on the removal of four selected (responses) parameters. The optimum removal efficiency for COD, TSS, color, and NH3-N was 87.15%, 65.54%, 52.78%, and 91.75%, respectively, using a POME/leachate mixing ratio of 188.32 mL/811.68 mL and 21 days of aeration time. The results demonstrate that POME-based agricultural waste can be effectively employed for organic removal from leachate.


2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Hathaikarn Thongpan ◽  
Rachadaporn Thongnan ◽  
Nirattisai Rakmak ◽  
Chairat Siripatana

In this work, both models for batch and continuous anaerobic digestion of palm oil mill effluent were developed based on Monod’s kinetics. Then the authors attempt to understand the effect of wastewater-sludge (WW:S) ratio on the biogas production efficiency in batch digesters. The experiments were carried out at a controlled temperature of 35±0.5 °C. Two series of the experiment were conducted. In the first series, the wastewater-sludge ratios covered 1:1 (add sodium bi-carbonate), 1:1, 1:2 and 2:1. It was found that the ratio of 1:2 gave the highest biogas producing efficiency followed by the ratio 1:1 (add sodium bi-carbonate). At 1:1 ratio, sodium bi-carbonate addition was required to start anaerobic digestion at a workable pH range whereas at 1:2 ratio the initial pH is in the workable range without the need of its addition. However, at the ratio of 2:1 the starting pH was too low to adjust pH economically by adding sodium bi-carbonate. The second series was to confine experiments to a narrower ratio range, namely: 1:1 (add sodium bi-carbonate), 1:1.5, 1:2, 1:2.5. In both sets of experiment, the ratio 1:2 gave the best biogas production potential of 76.62 and 78.52 ml of biogas/g COD removed respectively. In all treatments, the process was able to remove more than 80% of wastewater initial COD. The modified Gompertz equation was used to estimate the maximum specific biogas production rate (MBPR or Rm/S0). It was also found that the ratio of 1:2 gave the best MBPR in both experimental series (26.87 ml biogas/g COD-day). A modified Monod-type Model was also developed to describe the microbial growth, substrate consumption and biogas production in continuous operation. In general, sludge recycle provided active biomass which can use the substrate in the wastewater instantly without significant lag phase or delay. Furthermore, continuous-flow model developed, with parameters estimated from batch experiments, predicted the experimental kinetics of the actual continuous experiments satisfactory.  


2016 ◽  
Vol 73 (11) ◽  
pp. 2704-2712 ◽  
Author(s):  
Mohammed J. K. Bashir ◽  
Tham Mau Han ◽  
Lim Jun Wei ◽  
Ng Choon Aun ◽  
Salem S. Abu Amr

As the ponding system used to treat palm oil mill effluent (POME) frequently fails to satisfy the discharge standard in Malaysia, the present study aimed to resolve this problem using an optimized electrocoagulation process. Thus, a central composite design (CCD) module in response surface methodology was employed to optimize the interactions of process variables, namely current density, contact time and initial pH targeted on maximum removal of chemical oxygen demand (COD), colour and turbidity with satisfactory pH of discharge POME. The batch study was initially designed by CCD and statistical models of responses were subsequently derived to indicate the significant terms of interactive process variables. All models were verified by analysis of variance showing model significances with Prob > F < 0.01. The optimum performance was obtained at the current density of 56 mA/cm2, contact time of 65 min and initial pH of 4.5, rendering complete removal of colour and turbidity with COD removal of 75.4%. The pH of post-treated POME of 7.6 was achieved, which is suitable for direct discharge. These predicted outputs were subsequently confirmed by insignificant standard deviation readings between predicted and actual values. This optimum condition also permitted the simultaneous removal of NH3-N, and various metal ions, signifying the superiority of the electrocoagulation process optimized by CCD.


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