scholarly journals Electrochemical Immunosensor for NT-proBNP Detection in Artificial Human Saliva: Heart Failure Biomedical Application

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1085
Author(s):  
Elkahina Ghedir ◽  
Abdoullatif Baraket ◽  
Souad Kouchar ◽  
Selma Rabai ◽  
Messaoud Benounis ◽  
...  
Keyword(s):  
Heart Failure ◽  
Electrochemical Impedance ◽  
Biomedical Application ◽  
Human Saliva ◽  
Electrochemical Immunosensor ◽  
Chemical Surface ◽  
Linear Detection ◽  
Chemical Surface Modification ◽  
Detection Response ◽  
Cyclic Voltammetery

Monitoring of circulating N-terminal proBNP (NT-proBNP) biomarkers is crucial for the diagnosis of people suffering from heart failure (HF). In this work, we describe a novel ultra-sensitive NT-proBNP immunosensor for NT-proBNP detection in artificial human saliva. The surface of the developed immunosensor based on gold working microelectrodes (WEs) was biofunctionalized through carboxyl diazonium to immobilize anti-NT-proBNP antibodies. The chemical surface modification of WEs was carried out by cyclic voltammetery CV whilst the quantification of NT-proBNP biomarkers was made by electrochemical impedance spectroscopy (EIS). The immunosensor has demonstrated a linear detection response within the range 1–20 pg/mL for NTproBNP detection in artificial human saliva with a good selectivity in the presence of other interferences.

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

Chemical surface modification for the improvement of the hydrogenation kinetics and poisoning resistance of TiFe

2011 ◽  
Vol 509 ◽  
pp. S770-S774 ◽  
Author(s):  
M. Williams ◽  
M.V. Lototsky ◽  
M.W. Davids ◽  
V. Linkov ◽  
V.A. Yartys ◽  
...  
Keyword(s):  
Surface Modification ◽  
Chemical Surface ◽  
Chemical Surface Modification ◽  
Hydrogenation Kinetics
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