scholarly journals Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5411 ◽  
Author(s):  
Zofia Cebula ◽  
Sabina Żołędowska ◽  
Karolina Dziąbowska ◽  
Marta Skwarecka ◽  
Natalia Malinowska ◽  
...  

The present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA). Sensor development was characterized by cyclic voltammetry (CV) and antigen detection by electrochemical impedance spectroscopy (EIS) measurements. Seven analyzed strains of Psl were verified as positive by the reference method (PCR) and this immunoassay, proving sensor specificity. Label-free electrochemical detection was in the linear range 1 × 103–1.2 × 105 CFU/mL (colony-forming unit) with an R2 coefficient of 0.992 and a detection limit (LOD) of 337 CFU/mL. The sensor did not interfere with negative probes like buffers and other bacteria. The assay was proven to be fast (10 min detection) and easy in preparation. The advantage was the simplicity and availability of the verified analyte (whole bacteria) as the method does not require sample pretreatment (e.g., DNA isolation). EIS biosensing technique was chosen as one of the simplest and most sensitive with the least destructive influence on the probes compared to other electrochemical methods.

2010 ◽  
Vol 25 (5) ◽  
pp. 1122-1129 ◽  
Author(s):  
T. Houssin ◽  
J. Follet ◽  
A. Follet ◽  
E. Dei-Cas ◽  
V. Senez

Batteries ◽  
2018 ◽  
Vol 4 (4) ◽  
pp. 58 ◽  
Author(s):  
Daniel Manschke ◽  
Thorsten Seipp ◽  
Sascha Berthold ◽  
K. Friedrich

The state-of-the-art functionality test of classic redox-flow-stacks measures the current–voltage characteristic with the technical electrolyte. This research paper aims to simplify the validation of redox flow batteries’ functionality by conducting electrochemical impedance spectroscopy (EIS) on redox flow stacks. Since the electrolyte used in the batteries is usually toxic and aggressive, it would be a significant simplification to verify the functionality with an alternative, non-toxic fluid. EIS measurements on batteries with larger sized electrodes, multiple cells, and different fluids were performed. It was demonstrated that all impedances are repeatable, thereby validating this procedure as a qualification method for full-size and complex batteries with an alternative fluid. EIS measurements were able to detect deliberately manipulated cells. This research uses three different analysis methods for the acquired data to identify errors. The respective approaches are, firstly, (1) a comparison of the Nyquist plots; secondly, (2) a comparison of the Bode plots; and thirdly, (3) a comparison of the calculated characteristic values of the equivalent circuits. The analysis found that all methods are suitable to detect errors in the batteries. Nevertheless, the bode-plot comparison method proves to be especially advantageous, because it enables a quantitative statement.


RSC Advances ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 6679-6685 ◽  
Author(s):  
Wenyong Hu ◽  
Xiaobo Min ◽  
Xinyu Li ◽  
Shengxiang Yang ◽  
Langbo Yi ◽  
...  

In this study, we developed a novel selective method for copper quantification based on gold nanoclusters (GNCs) and DNAzyme.


2009 ◽  
Vol 21 (20) ◽  
pp. 2267-2271 ◽  
Author(s):  
Jeffrey T. La Belle ◽  
Miti Shah ◽  
Justin Reed ◽  
Vivek Nandakumar ◽  
T. L. Alford ◽  
...  

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