Electrochemical Deoxyribonucleic Acid Biosensor Based on Multi-walled Carbon Nanotubes/Ag-TiO<SUB>2</SUB> Composite Film for Label-free Phosphinothricin Acetyltransferase Gene Detection by Electrochemical Impedance Spectroscopy

AbstractA highly sensitive DNA electrochemical biosensor was prepared based on multi-walled carbon nanotube/nano-Ag-TiO2 composite membrane. The Ag-TiO2 composite is mixed with a suitable amount of multi-walled carbon nanotubes (MWNT) dispersed in N,N-dimethylformamide to form a uniform and stable mixed solution, which is applied onto the surface of the bare carbon paste electrode. A MWNT/Ag-TiO2 modified carbon paste electrode was prepared. The large specific surface area and good electron transport properties of carbon nanotubes have a good synergistic effect on the good biocompatibility of Ag-TiO2 nanocomposites and excellent adsorption capacity of DNA, which significantly improves the immobilization and DNA hybridization of DNA probes. Detection sensitivity. The preparation of the sensing membrane and the immobilization and hybridization of DNA were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The exogenous glufosinate acetyltransferase gene fragment of transgenic plants was detected by electrochemical impedance spectroscopy. The linear range was 1. 0 × 10 - 11 ∼1. 0 × 10 - 6 mol / L. The detection limit was 3. 12 × 10 - 12 mol / L.

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Effects of carboxylated multi-walled carbon nanotubes having different outer diameters on hollow fiber ultrafiltration membrane fabrication and characterization by electrochemical impedance spectroscopy

Polymer Bulletin ◽

10.1007/s00289-017-2155-3 ◽

2017 ◽

Vol 75 (6) ◽

pp. 2431-2457 ◽

Cited By ~ 6

Author(s):

Esra A. Genceli ◽

Reyhan Sengur-Tasdemir ◽

Gulsum Melike Urper ◽

Selin Gumrukcu ◽

Zeliha Guler-Gokce ◽

...

Keyword(s):

Carbon Nanotubes ◽

Electrochemical Impedance Spectroscopy ◽

Impedance Spectroscopy ◽

Hollow Fiber ◽

Electrochemical Impedance ◽

Ultrafiltration Membrane ◽

Membrane Fabrication ◽

Multi Walled Carbon Nanotubes ◽

Fabrication And Characterization ◽

Walled Carbon Nanotubes

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Detection and discrimination of alpha-fetoprotein with a label-free electrochemical impedance spectroscopy biosensor array based on lectin functionalized carbon nanotubes

Talanta ◽

10.1016/j.talanta.2013.01.060 ◽

2013 ◽

Vol 111 ◽

pp. 62-68 ◽

Cited By ~ 41

Author(s):

Haiying Yang ◽

Zhejian Li ◽

Xiaomin Wei ◽

Ru Huang ◽

Honglan Qi ◽

...

Keyword(s):

Carbon Nanotubes ◽

Electrochemical Impedance Spectroscopy ◽

Impedance Spectroscopy ◽

Electrochemical Impedance ◽

Alpha Fetoprotein ◽

Label Free ◽

Functionalized Carbon Nanotubes ◽

Biosensor Array

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Disposable Multi-Walled Carbon Nanotubes-Based Plasticizer-Free Solid-Contact Pb2+-Selective Electrodes with a Sub-PPB Detection Limit †

Sensors ◽

10.3390/s19112550 ◽

2019 ◽

Vol 19 (11) ◽

pp. 2550 ◽

Cited By ~ 2

Author(s):

Yueling Liu ◽

Yingying Gao ◽

Rui Yan ◽

Haobo Huang ◽

Ping Wang

Keyword(s):

Carbon Nanotubes ◽

Electrochemical Impedance Spectroscopy ◽

Detection Limit ◽

Electrochemical Impedance ◽

Solid Contact ◽

Water Films ◽

Multi Walled Carbon Nanotubes ◽

Nernstian Slope ◽

Disk Electrodes ◽

Walled Carbon Nanotubes

Potentiometric plasticizer-free solid-contact Pb2+-selective electrodes based on copolymer methyl methacrylate-n-butyl acrylate (MMA-BA) as membrane matrix and multi-walled carbon nanotubes (MWCNTs) as intermediate ion-to-electron transducing layer have been developed. The disposable electrodes were prepared by drop-casting the copolymer membrane onto a layer of MWCNTs, which deposited on golden disk electrodes. The obtained electrodes exhibited a sub-ppb level detection limit of 10−10 mol·L−1. The proposed electrodes demonstrated a Nernstian slope of 29.1 ± 0.5 mV/decade in the linear range from 2.0 × 10−10 to 1.5 × 10−3 mol·L−1. No interference from gases (O2 and CO2) or water films was observed. The electrochemical impedance spectroscopy of the fabricated electrodes was compared to that of plasticizer-free Pb2+-selective electrodes without MWCNTs as intermediated layers. The plasticizer-free MWCNTs-based Pb2+-selective electrodes can provide a promising platform for Pb(II) detection in environmental and clinical application.

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