scholarly journals Detection of Particulate Matters with a Field-Portable Microscope Using Side-Illuminated Total Internal Reflection

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2745
Author(s):  
Haechang Yang ◽  
Sanghoon Shin ◽  
Dongmin Seo ◽  
Jaewon Park ◽  
Sungkyu Seo
Keyword(s):  
Total Internal Reflection ◽  
Detection System ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Internal Reflection ◽  
Dust Particles ◽  
Halogen Lamp ◽  
Signal To Noise ◽  
Portable Detection ◽  
Transmission Microscope

Field-portable observation and analysis of particulate matter (PM) is required to enhance healthy lives. Various types of the PM measurement methods are in use; however, each of these methods has significant limitations in that real time measurement is impossible, the detection system is bulky, or the measurement accuracy is insufficient. In this work, we introduce an optical method to perform a fast and accurate PM analysis with a higher-contrast microscopic image enabled by a side-illuminated total internal reflection (TIR) technique to be implemented in a compact device. The superiority of the proposed method was quantitatively demonstrated by comparing the signal-to-noise ratio of the proposed side-illuminated TIR method with a traditional halogen lamp-based transmission microscope. With the proposed device, signal-to-noise ratios (SNRs) for microbeads (5~20 µm) and ultrafine dust particles (>5 µm) increased 4.5~17 and 4~10 dB, respectively, compared to the conventional transmission microscope. As a proof of concept, the proposed method was also applied to a low-cost commercial smartphone toy microscope enabling field-portable detection of PMs. We believe that the proposed side-illuminated TIR PM detection device holds significant advantages over other commonly used systems due to its sufficient detection capability along with simple and compact configuration as well as low cost.

scholarly journals LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

2021 ◽  
Author(s):  
Bo YANG ◽  
zhengwang shi ◽  
Yuan Ma ◽  
Lijuan Wang ◽  
Liyan Cao ◽  
...  
Keyword(s):  
Real Time ◽  
Detection System ◽  
African Swine Fever Virus ◽  
Low Cost ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Cross Reactivity ◽  
Clinical Samples ◽  
Portable Detection ◽  
Real Time Qpcr

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a LAMP assay coupled with the CRISPR Cas12a system was established in one tube for the detection of the ASFV p72 gene. The single-strand DNA-fluorophore-quencher (ssDNA-FQ) reporters and CRISPR-derived RNA (crRNAs) were screened and selected for the CRISPR detection system. In combination with LAMP amplification assay, the detection limit for the LAMP-CRISPR assay can reach 7 copies/μl of p72 gene per reaction. Furthermore, this method displays no cross-reactivity with other porcine DNA or RNA viruses. The performance of the LAMP-CRISPR assay was compared with real-time qPCR tests for clinical samples, a good consistency between the LAMP-CRISPR assay and real-time qPCR was observed. In the current study, a LAMP coupled with the CRISPR detection method was developed. The method shed a light on the convenient, portable, low cost, highly sensitive and specific detection of ASFV, demonstrating a great application potential for monitoring on-site ASFV in the field.

Internally Modulated Chirped Pulse Based Direct Detection Type φ-OTDR System with High Signal-to-Noise Ratio and Low Cost

2019 ◽  
Vol 46 (8) ◽  
pp. 0806003
Author(s):  
李鲁川 Luchuan Li ◽  
卢斌 Bin Lu ◽  
王校 Xiao Wang ◽  
梁嘉靖 Jiajing Liang ◽  
郑汉荣 Hanrong Zheng ◽  
...  
Keyword(s):  
Direct Detection ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
High Signal ◽  
Signal To Noise ◽  
Chirped Pulse ◽  
Noise Ratio

scholarly journals Signal-to-noise optimization and evaluation of a home-made visible diode-array spectrophotometer

1993 ◽  
Vol 15 (6) ◽  
pp. 227-232 ◽  
Author(s):  
Ivo M. Raimundo, Jr. ◽  
Celio Pasquini
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Optical Design ◽  
Signal To Noise ◽  
Noise Optimization ◽  
Instrument Control ◽  
Automatic Methods ◽  
Photodiode Array ◽  
Variable Integration

This paper describes a simple low-cost multichannel visible spectrophotometer built with an RL512G EGG-Reticon photodiode array. A symmetric Czerny-Turner optical design was employed; instrument control was via a single-board microcomputer based on the 8085 Intel microprocessor. Spectral intensity data are stored in the single-board's RAM and then transferred to an IBM-AT 3865X compatible microcomputer through a RS-232C interface. This external microcomputer processes the data to recover transmittance, absorbance or relative intensity of the spectra. The signal-to-noise ratio and dynamic range were improved by using variable integration times, which increase during the same scan; and by the use of either weighted or unweighted sliding average of consecutive diodes. The instrument is suitable for automatic methods requiring quasi-simultaneous multiwavelength detections, such as multivariative calibration and flow-injection gradient scan techniques.

A constrained minimum power adaptive beamformer with time‐varying adaptation rate

Geophysics ◽  
1979 ◽  
Vol 44 (6) ◽  
pp. 1088-1096 ◽  
Author(s):  
Wen‐Wu Shen
Keyword(s):  
Adaptive Algorithm ◽  
Detection System ◽  
Signal To Noise Ratio ◽  
High Amplitude ◽  
Research Station ◽  
Power Performance ◽  
Signal To Noise ◽  
Filter Output ◽  
Short Period ◽  
Noise Ratio

A linear adaptive algorithm was developed for array beamforming purposes. The design goal for the algorithm is to minimize the squared filter output subject to filter constraints which allow energy propagating from the array steering direction to pass without being distorted. The adaptive filter coefficients were initialized to satisfy the constraints which were preserved during the iterations. The adaptation rate is inversely varied with filter output and total input channel power. Performance of the algorithm was studied using the recorded short‐period array data from the Korean Seismic Research Station. Processed were a high‐amplitude signal from Kamchatka, a medium‐amplitude signal from eastern Kazakh, and a number of low‐amplitude signals from central Eurasia. Results of signal‐to‐noise ratio gain relative to a conventional beamformer among the events tested were consistent and were in the range of 4.5 to 6.5 dB in the wide passband. Much better signal‐to‐noise ratio improvement was obtained in the low‐frequency passband. The adaptive algorithm was programmed in the real‐time mode and can be implemented in a front‐end detection system.

A Motorized Microdrive for Recording of Neural Ensembles in Awake Behaving Rats

2005 ◽  
Vol 127 (6) ◽  
pp. 1035-1040 ◽  
Author(s):  
R. Venkateswaran ◽  
Chris Boldt ◽  
J. Parthasarathy ◽  
B. Ziaie ◽  
A. G. Erdman ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Low Cost ◽  
Dynamic Control ◽  
Experimental Paradigm ◽  
Signal To Noise ◽  
Implanted Electrodes ◽  
Neural Ensembles ◽  
Dc Motors ◽  
Cell Electrode ◽  
Scientific Advances

The recording of neural ensembles in awake, behaving rats has been an extremely successful experimental paradigm, providing demonstrable scientific advances. Dynamic control of the position of the implanted electrodes is of key importance as mobile electrodes provide a better signal-to-noise ratio and a better cell/electrode yield than nonmobile electrodes. Here we describe the use of low cost, soon to be commercially available dc motors to successfully control the depth of electrodes. The prototype designed is approximately 30mm in diameter and 50mm in length and weighed about 30gms. This paper presents the results of linear displacements of electrodes achievable with this motorized microdrive.

An Analysis of Infrared Spectroscopic Geometries

1997 ◽  
Vol 477 ◽  
Author(s):  
Gregory T. Merklin ◽  
Huihong Luo ◽  
Christopher E. D. Chidsey
Keyword(s):  
Vibrational Mode ◽  
Signal To Noise Ratio ◽  
Signal Strength ◽  
Internal Reflection ◽  
Infrared Spectrometry ◽  
Vibrational Modes ◽  
Signal To Noise ◽  
Transmission Spectroscopy ◽  
Infrared Spectroscopic ◽  
Experimental Geometry

ABSTRACTThe influence of experimental geometry on the signal strength and signal-to-noise ratio of infrared spectrometry has been investigated. In general, it was found that the choice of optimum experimental geometry depended on the orientation of the vibrational mode being investigated. In particular, it has been calculated that internal reflection spectrometry is relatively insensitive to vibrational modes perpendicular to the surface relative to transmission spectroscopy at Brewster's angle, and this has been confirmed by experiment.

Quantitative Analysis of Noise Impact on Duffing Chaotic Detection System Using Lyapunov Characteristic Exponents

2011 ◽  
Vol 130-134 ◽  
pp. 1331-1337
Author(s):  
Wen Jing Hu ◽  
Zhi Zhen Liu ◽  
Zhi Hui Li
Keyword(s):  
Detection System ◽  
Signal To Noise Ratio ◽  
Duffing Oscillator ◽  
Threshold Value ◽  
System State ◽  
Signal To Noise ◽  
Phase Trajectories ◽  
Lyapunov Characteristic Exponents ◽  
Noise Ratio ◽  
System States

Performance of the Duffing oscillator to detect weak signals buried in heavy noise is analyzed quantitatively by LCEs. First in the case of noise, differential equations to compute LCE s are derived using RHR algorithm, so the quantitative criteria to identify system states are obtained. Then using LCEs, the threshold value of the forced periodic term is found accurately. Finally the system state and state change are analyzed using LCEs by keeping the threshold value and varying the noise intensity, and the minimum signal to noise ratio is determined. By contrast of phase trajectories and LCEs, it shows that phase trajectories disturbed by strong noise sometimes are ambiguous to our eyes, but through LCEs, the system state can be identified clearly and quantitatively especially in strong noise background. So the minimum signal to noise ratio can be obtained accurately.

scholarly journals Comparing current noise in biological and solid-state nanopores

2019 ◽  
Author(s):  
A. Fragasso ◽  
S. Schmid ◽  
C. Dekker
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Ionic Current ◽  
Low Noise ◽  
Signal To Noise ◽  
Experimental Conditions ◽  
High Frequencies ◽  
Noise Ratio

AbstractNanopores bear great potential as single-molecule tools for bioanalytical sensing and sequencing, due to their exceptional sensing capabilities, high-throughput, and low cost. The detection principle relies on detecting small differences in the ionic current as biomolecules traverse the nanopore. A major bottleneck for the further progress of this technology is the noise that is present in the ionic current recordings, because it limits the signal-to-noise ratio and thereby the effective time resolution of the experiment. Here, we review the main types of noise at low and high frequencies and discuss the underlying physics. Moreover, we compare biological and solid-state nanopores in terms of the signal-to-noise ratio (SNR), the important figure of merit, by measuring free translocations of a short ssDNA through a selected set of nanopores under typical experimental conditions. We find that SiNx solid-state nanopores provide the highest SNR, due to the large currents at which they can be operated and the relatively low noise at high frequencies. However, the real game-changer for many applications is a controlled slowdown of the translocation speed, which for MspA was shown to increase the SNR >160-fold. Finally, we discuss practical approaches for lowering the noise for optimal experimental performance and further development of the nanopore technology.

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