Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30–50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive; 65% were hydrogen cyanide (HCN) positive; 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains.

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Biosurfactant based formulation of Pseudomonas guariconensis LE3 with multifarious plant growth promoting traits controls charcoal rot disease in Helianthus annus

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-021-03015-4 ◽

2021 ◽

Vol 37 (4) ◽

Author(s):

Ekta Khare ◽

Naveen Kumar Arora

Keyword(s):

Plant Growth ◽

Charcoal Rot ◽

Helianthus Annus ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Rot Disease ◽

Plant Growth Promoting Traits

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Biosurfactant-mediated Biocontrol of Macrophomina phaseolina Causing Charcoal Rot in Vigna mungo by a Plant Growth Promoting Enterococcus sp. BS13

Journal of Plant Pathology & Microbiology ◽

10.4172/2157-7471.1000385 ◽

2016 ◽

Vol 7 (11) ◽

Cited By ~ 1

Author(s):

Sumit Kumar ◽

Dubey RC ◽

Maheshwari DK

Keyword(s):

Plant Growth ◽

Macrophomina Phaseolina ◽

Vigna Mungo ◽

Charcoal Rot ◽

Plant Growth Promoting ◽

Growth Promoting

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Exploiting plant growth-promoting Amycolatopsis sp. for bio-control of charcoal rot of sorghum (Sorghum bicolor L.) caused by Macrophomina phaseolina (Tassi) Goid

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2018.1553472 ◽

2019 ◽

Vol 52 (7-8) ◽

pp. 543-559

Author(s):

S. Gopalakrishnan ◽

V. Srinivas ◽

N. Naresh ◽

G. Alekhya ◽

R. Sharma

Keyword(s):

Plant Growth ◽

Sorghum Bicolor ◽

Macrophomina Phaseolina ◽

Charcoal Rot ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Sorghum Bicolor L

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Sulfur Application Combined with Planomicrobium sp. Strain MSSA-10 and Farmyard Manure Biochar Helps in the Management of Charcoal Rot Disease in Sunflower (Helianthus annuus L.)

Sustainability ◽

10.3390/su13158535 ◽

2021 ◽

Vol 13 (15) ◽

pp. 8535

Author(s):

Muhammad Ijaz ◽

Abdul Sattar ◽

Ahmad Sher ◽

Sami Ul-Allah ◽

Muhammad Zeeshan Mansha ◽

...

Keyword(s):

Plant Growth ◽

Helianthus Annuus ◽

Farmyard Manure ◽

Growth Yield ◽

Plant Growth Promoting Rhizobacteria ◽

Charcoal Rot ◽

Helianthus Annuus L ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Rot Disease

Sunflower (Helianthus annuus L.), a member of the Asteraceae, is one of the major oilseed crops around the world. Charcoal rot caused by Macrophomina phaseolina (Tassi) Goid is the most damaging disease of sunflowers globally. Fungicides are mostly used to control charcoal rot; however, these cause environmental pollution and pose adverse effects on the ecosystem. Therefore, ecofriendly management options are inevitable for the management of charcoal rot disease. Plant mineral nutrition, the use of plant growth-promoting rhizobacteria and biochar have recently been manipulated for the management of different plant diseases. However, the interactive effects of all these treatments have rarely been tested on charcoal rot suppression in sunflowers. This study assessed the influence of sulfur (0 and 2.25 mg/kg) combined with farmyard manure biochar (2%), NPK (20:20:20 mg/kg) and three different plant growth-promoting rhizobacteria (PGPR) strains on the charcoal rot suppression growth, yield, biochemistry and physiology of sunflower. The PGPR strains included in the study were Bacillus sp. strain MR-1/2 (regarded as PGPR1), Achromobacter sp. strain FB-14 (regarded as PGPR2) and Planomicrobium sp. strain MSSA-10 (regarded as PGPR3). The charcoal rot infestation was induced by inoculating the soil with M. phaseolina, and the impacts of the different treatments were studied on the disease infestation, growth, yield, biochemistry and physiology of sunflowers under 0 and 2.25-mg/kg S application. The results revealed that farmyard manure biochar and Planomicrobium sp. strain MSSA-10 in combination with 2.25-mg/kg S proved effective for the management of charcoal rot disease through regulating the antioxidant enzymes’ activities and strengthening the immune system of sunflower plants. The studied health markers (total chlorophyll content and carotenoids) and stress markers (total protein content, catalase and peroxidase) were significantly altered by the applied treatments under 0 and 2.25-mg/kg S applications. The findings of the experiment indicated that both farmyard manure biochar and Planomicrobium sp. strain MSSA-10, combined with 2.25-mg/kg S, could be used to enhance the crop yield and manage charcoal rot disease in sunflowers. Farmyard manure biochar and Planomicrobium sp. strain MSSA-10 are an easy-to-apply, cost-effective, ecofriendly and sustainable option for the management of charcoal rot disease in sunflowers.

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Systemic Disease Protection Elicited by Plant Growth Promoting Rhizobacteria Strains: Relationship Between Metabolic Responses, Systemic Disease Protection, and Biotic Elicitors

Phytopathology ◽

10.1094/phyto-98-4-0451 ◽

2008 ◽

Vol 98 (4) ◽

pp. 451-457 ◽

Cited By ~ 68

Author(s):

B. Ramos Solano ◽

J. Barriuso Maicas ◽

M. T. Pereyra de la Iglesia ◽

J. Domenech ◽

F. J. Gutiérrez Mañero

Keyword(s):

Salicylic Acid ◽

Plant Growth ◽

Disease Severity ◽

Systemic Disease ◽

Plant Growth Promoting Rhizobacteria ◽

Disease Suppression ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Systemic Responses ◽

Dependent Pathway

A study of plant defensive systemic responses induced by three plant growth promoting rhizobacteria (PGPR) on Arabidopsis thaliana Col 0 against Pseudomonas syringae pv. tomato DC3000 at the biochemical and transcriptional levels is reported in this paper. All three strains decreased disease severity when applied to A. thaliana prior to pathogen inoculation. At the biochemical level, each of the three strains induced ethylene (ET) when incubated with 1-amino-cyclopropane-1-carboxylic acid, and salicylic acid (SA) production in the plant. Plants treated with each of the three strains were also reduced in salicylic acid production after pathogen challenge compared to untreated controls. This effect was more marked in plants treated with Chryseobacterium balustinum AUR9, the strain most effective in decreasing disease severity. The expression level of PR1, a transcriptional marker of the SA-dependent pathway in C. balustinum AUR9-treated plants, is fourfold that of controls while the expression of PDF1.2, a transcriptional marker for the SA-independent pathway, is not induced. C. balustinum cell wall lipopolysaccharides, being putative bacterial elicitor molecules, are able to reproduce this systemic induction effect at low doses. From these observations, we hypothesize that certain PGPR strains are capable of stimulating different systemic responses in host plants. With C. balustinum AUR9, the SA-dependent pathway is stimulated first, as indicated by increases in SA levels and PR1 expression, followed by induction of the SA-independent pathway, as indicated by the increases in ET concentrations. The effects of both pathways combined with respect to disease suppression appear to be additive.

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