Exploiting plant growth-promoting Amycolatopsis sp. for bio-control of charcoal rot of sorghum (Sorghum bicolor L.) caused by Macrophomina phaseolina (Tassi) Goid

Plant growth promoting rhizobacteria are the bacteria which subsist inside and outside of the plant tissue and promote plant growth through direct or indirect mechanisms. To increase sorghum production and productivity we utilize herbicides and chemical fertilizers to overcome sorghum production constraints, but those chemicals have negative side effects. The current study was conducted with the objective of isolation of PGPR from sorghum rhizosphere and screening for primary growth related trait, evaluation of potential PGPR at greenhouse for sorghum growth performance and identify through biochemical characterization. So that, in this study a total of 117 plant growth promoting rhizobacteria were isolated from the rhizosphere of 12 sorghum (Sorghum bicolor L. Moench) genotype by cultivating using 3 collected soil samples from the northern part of Ethiopia (Amhara and Tigray regional states) in greenhouse. Isolated bacteria were screened for primary growth promoting traits such as phosphate solubilization test, IAA production test at different concentration of L-tryptophan and ammonia production test. From the isolated bacteria 28% solubilized Phosphorous, 78% produced IAA at different concentration of tryptophan. The greatest IAA production was scored at 100 mg/L of tryptophan and the lowest production of IAA was scored at 150 mg/L of tryptophan, 69% of isolated bacteria produced ammonia. Hence, 15% of isolated bacteria fulfilled the above primary screening test and used for further greenhouse evaluation. Accordingly, eighteen bacteria were tested for greenhouse experiment using completely randomized design and all 18 isolates were significantly increased all the agronomic parameter as compared to the control such as plant shoot height, plant shoot fresh and dry weight, root length, root fresh and dry weight at p < 0.01 and P ≤ 0.001. Two isolates G6E29 and G4E19 had significantly increased all the parameter but two isolates (G12E19 and G3E40) were statistically non-significant for root fresh weight compared to the control. These 18 potential isolates were characterized morphologically and biochemically. Eight isolates were grouped at Pseudomonas genera. Six isolates were grouped at Azotobacter and the rest four isolates were grouped at Bacillus genera. Thus, the use of plant growth promoting rhizosphere bacteria could be useful to improve sorghum production and productivity. However, further molecular identification and evaluation of the isolates exhibiting multiple plant growths promoting traits on plant-microbe interaction for economic crop of Ethiopia is needed to uncover their efficacy as effective plant growth promoting rhizosphere bacteria.

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The influence of some Bradyrhizobium and Rhizobium strains as plant growth promoting rhizobacteria on the growth and yield of sorghum (Sorghum bicolor L.) plants under drought stress

Plant Nutrition ◽

10.1007/0-306-47624-x_322 ◽

2001 ◽

pp. 664-665 ◽

Cited By ~ 1

Author(s):

M. H. Rashad ◽

A. A. Ragab ◽

S. M. Salem

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Sorghum Bicolor ◽

Plant Growth Promoting Rhizobacteria ◽

Growth And Yield ◽

Rhizobium Strains ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Sorghum Bicolor L

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Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina phaseolina in Soybean (Glycine max L.)

Sustainability ◽

10.3390/su12176856 ◽

2020 ◽

Vol 12 (17) ◽

pp. 6856

Author(s):

Humaira Yasmin ◽

Rabia Naz ◽

Asia Nosheen ◽

Muhammad Nadeem Hassan ◽

Noshin Ilyas ◽

...

Keyword(s):

Salicylic Acid ◽

Plant Growth ◽

Jasmonic Acid ◽

Macrophomina Phaseolina ◽

Disease Suppression ◽

Charcoal Rot ◽

Bacillus Clausii ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Rot Disease

Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30–50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive; 65% were hydrogen cyanide (HCN) positive; 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains.

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Characterization & identification endophytic bacteria from Sorghum bicolor (L.) Moench as plant growth promoting bacteria

International Journal of Scientific and Engineering Research ◽

10.14299/ijser.2019.07.09 ◽

2019 ◽

Vol 10 (7) ◽

pp. 929-933

Author(s):

Charlie Ester de Fretes ◽

Donny Widianto ◽

Yekti Asih Purwestri ◽

Tri Rini Nuringtyas

Keyword(s):

Plant Growth ◽

Sorghum Bicolor ◽

Endophytic Bacteria ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Sorghum Bicolor L

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Selection of plant growth-promoting bacteria in sweet sorghum (Sorghum bicolor (L.) Moench) under the effects of salinity

BMC Proceedings ◽

10.1186/1753-6561-8-s4-p113 ◽

2014 ◽

Vol 8 (S4) ◽

Cited By ~ 1

Author(s):

Marta Amâncio do Nascimento ◽

Carlos Vinicius do Nascimento ◽

Jadson Emanuel Antunes ◽

Márcia do Vale Figueiredo ◽

José Nildo Tabosa ◽

...

Keyword(s):

Plant Growth ◽

Sorghum Bicolor ◽

Sweet Sorghum ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Sorghum Bicolor L ◽

Selection Of

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Biosurfactant based formulation of Pseudomonas guariconensis LE3 with multifarious plant growth promoting traits controls charcoal rot disease in Helianthus annus

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-021-03015-4 ◽

2021 ◽

Vol 37 (4) ◽

Author(s):

Ekta Khare ◽

Naveen Kumar Arora

Keyword(s):

Plant Growth ◽

Charcoal Rot ◽

Helianthus Annus ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Rot Disease ◽

Plant Growth Promoting Traits

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Plant growth promoting endophyte Burkholderia contaminans NZ antagonizes phytopathogen Macrophomina phaseolina through melanin synthesis and pyrrolnitrin inhibition

PLoS ONE ◽

10.1371/journal.pone.0257863 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0257863

Author(s):

Nazia R. Zaman ◽

Umar F. Chowdhury ◽

Rifath N. Reza ◽

Farhana T. Chowdhury ◽

Mrinmoy Sarker ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Genome Mining ◽

Gene Clusters ◽

Macrophomina Phaseolina ◽

Melanin Synthesis ◽

Burkholderia Contaminans ◽

Plant Growth Promoting ◽

Growth Promoting

The endophytic bacterium Burkholderia contaminans NZ was isolated from jute, which is an important fiber-producing plant. This bacterium exhibits significant growth promotion activity in in vivo pot experiments, and like other plant growth-promoting (PGP) bacteria fixes nitrogen, produces indole acetic acid (IAA), siderophore, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. B. contaminans NZ is considered to exert a promising growth inhibitory effect on Macrophomina phaseolina, a phytopathogen responsible for infecting hundreds of crops worldwide. This study aimed to identify the possibility of B. contaminans NZ as a safe biocontrol agent and assess its effectiveness in suppressing phytopathogenic fungi, especially M. phaseolina. Co-culture of M. phaseolina with B. contaminans NZ on both solid and liquid media revealed appreciable growth suppression of M. phaseolina and its chromogenic aberration in liquid culture. Genome mining of B. contaminans NZ using NaPDoS and antiSMASH revealed gene clusters that displayed 100% similarity for cytotoxic and antifungal substances, such as pyrrolnitrin. GC-MS analysis of B. contaminans NZ culture extracts revealed various bioactive compounds, including catechol; 9,10-dihydro-12’-hydroxy-2’-methyl-5’-(phenylmethyl)- ergotaman 3’,6’,18-trione; 2,3-dihydro-3,5- dihydroxy-6-methyl-4H-pyran-4-one; 1-(1,6-Dioxooctadecyl)- pyrrolidine; 9-Octadecenamide; and 2- methoxy- phenol. These compounds reportedly exhibit tyrosinase inhibitory, antifungal, and antibiotic activities. Using a more targeted approach, an RP-HPLC purified fraction was analyzed by LC-MS, confirming the existence of pyrrolnitrin in the B. contaminans NZ extract. Secondary metabolites, such as catechol and ergotaman, have been predicted to inhibit melanin synthesis in M. phaseolina. Thus, B. contaminans NZ appears to inhibit phytopathogens by apparently impairing melanin synthesis and other potential biochemical pathways, exhibiting considerable fungistatic activity.

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