scholarly journals A Case of In Situ Phage Therapy against Staphylococcus aureus in a Bone Allograft Polymicrobial Biofilm Infection: Outcomes and Phage-Antibiotic Interactions

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1898
Author(s):  
Brieuc Van Nieuwenhuyse ◽  
Christine Galant ◽  
Bénédicte Brichard ◽  
Pierre-Louis Docquier ◽  
Sarah Djebara ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phage Therapy ◽  
Bone Allograft ◽  
Pelvic Bone ◽  
Graft Replacement ◽  
Incomplete Coverage ◽  
Antibiotic Interactions ◽  
Case Basis ◽  
Biofilm Infection

Phage therapy (PT) shows promising potential in managing biofilm infections, which include refractory orthopedic infections. We report the case of a 13-year-old girl who developed chronic polymicrobial biofilm infection of a pelvic bone allograft after Ewing’s sarcoma resection surgery. Chronic infection by Clostridium hathewayi, Proteus mirabilis and Finegoldia magna was worsened by methicillin-susceptible Staphylococcus aureus exhibiting an inducible Macrolides-Lincosamides-Streptogramin B resistance phenotype (iMLSB). After failure of conventional conservative treatment, combination of in situ anti-S. aureus PT with surgical debridement and intravenous antibiotic therapy led to marked clinical and microbiological improvement, yet failed to prevent a recurrence of infection on the midterm. This eventually led to surgical graft replacement. Multiple factors can explain this midterm failure, among which incomplete coverage of the polymicrobial infection by PT. Indeed, no phage therapy against C. hathewayi, P. mirabilis or F. magna could be administered. Phage-antibiotic interactions were investigated using OmniLog® technology. Our results suggest that phage-antibiotic interactions should not be considered “unconditionally synergistic”, and should be assessed on a case-by-case basis. Specific pharmacodynamics of phages and antibiotics might explain these differences. More than two years after final graft replacement, the patient remains cured of her sarcoma and no further infections occurred.

5-Benzylidene-4-Oxazolidinones are Synergistic with Antibiotics for the Treatment of Staphylococcus Aureus Biofilms

2019 ◽  
Author(s):  
Bram Frohock ◽  
Jessica M. Gilbertie ◽  
Jennifer C. Daiker ◽  
Lauren V. Schnabel ◽  
Joshua Pierce
Keyword(s):  
Staphylococcus Aureus ◽  
Human Health ◽  
Matrix Model ◽  
Bacterial Load ◽  
Collagen Matrix ◽  
Resistance Mechanisms ◽  
Novel Therapeutics ◽  
Innovative Treatment ◽  
Antibiotic Action ◽  
Biofilm Infection

<div>The failure of frontline antibiotics in the clinic is one of the most serious threats to human health and requires a multitude of novel therapeutics and innovative treatment approaches to curtail the growing crisis. In addition to traditional resistance mechanisms resulting in the lack of efficacy of many antibiotics, most chronic and recurring infections are further made tolerant to antibiotic action by the presence of biofilms. Herein, we report an expanded set of 5-benzylidene-4-oxazolidinones that are able to inhibit the formation of Staphylococcus aureus biofilms, disperse preformed biofilms and in combination with common antibiotics are able to significantly reduce the bacterial load in a robust collagen-matrix model of biofilm infection.</div>

scholarly journals In situ regeneration of bioactive coatings enabled by an evolved Staphylococcus aureus sortase A

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Hyun Ok Ham ◽  
Zheng Qu ◽  
Carolyn A. Haller ◽  
Brent M. Dorr ◽  
Erbin Dai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A ◽  
Bioactive Coatings ◽  
In Situ Regeneration

The Results of In Situ Prosthetic Graft Replacement for Infected Aortic Disease

2018 ◽  
Vol 42 (9) ◽  
pp. 3035-3041 ◽  
Author(s):  
Youngjin Han ◽  
Tae-Won Kwon ◽  
Sang Jun Park ◽  
Min-Jae Jeong ◽  
Kyunghak Choi ◽  
...  
Keyword(s):  
Prosthetic Graft ◽  
Aortic Disease ◽  
Graft Replacement

scholarly journals Hybrid in situ replacement for Samson group V Staphylococcus aureus aortic graft infection

2013 ◽  
Vol 2013 (jul29 1) ◽  
pp. bcr2013010289-bcr2013010289 ◽  
Author(s):  
A. A. Karpenko ◽  
P. V. Ignatenko ◽  
A. M. Beliaev
Keyword(s):  
Staphylococcus Aureus ◽  
Graft Infection ◽  
Aortic Graft ◽  
Group V ◽  
Aortic Graft Infection ◽  
In Situ Replacement

Repurposing of antidepression drug sertraline for antimicrobial activity against Staphylococcus aureus: a potential approach for the treatment of osteomyelitis

2019 ◽  
Vol 43 (14) ◽  
pp. 5315-5324 ◽  
Author(s):  
D. Muthu ◽  
M. Gowri ◽  
G. Suresh Kumar ◽  
V. S. Kattimani ◽  
E. K. Girija
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Precipitation Method ◽  
Hydroxyapatite Nanoparticles ◽  
Potential Approach ◽  
Calcium Source

We report a potential approach to synthesize the repurposed sertraline drug-loaded hydroxyapatite nanoparticles using eggshell as the calcium source via the in situ precipitation method for the treatment of osteomyelitis.

Localization and Orientation of Subunit Delta of Spinach Chloroplast ATP-Synthase within the CF0CF1 Complex. 1. Distinction of Shielded and Exposed Surfaces of Delta on the Thylakoid Membrane

1989 ◽  
Vol 44 (1-2) ◽  
pp. 153-160 ◽  
Author(s):  
Richard J. Berzborn ◽  
Werner Finke
Keyword(s):  
Staphylococcus Aureus ◽  
Atp Synthase ◽  
Thylakoid Membrane ◽  
Quaternary Structure ◽  
Large Degree ◽  
Polyclonal Antiserum ◽  
Spinach Chloroplast ◽  
Cyclic Photophosphorylation ◽  
Exposed Part

Abstract A new polyclonal antiserum against spinach CF1 subunit delta was produced in rabbits. It decorates only one band at 21 kDa in Western immunoblots of thylakoid proteins and does not react in ELISA with δ-free four subunit CF1 (-δ); therefore it is regarded monospecific. The polypeptide used as immunogen had been purified by HPLC. Earlier antisera against CF1 δ crossreact with CF1 subunit β. The new antiserum 306 contains different antibodies; some can be absorbed with thylakoids, i.e. by δ within the assembled CF0CF1 complex on the membrane, others still react in ELISA with isolated CF1. The former antibodies agglutinate thylakoids and inhibit PMS cyclic photophosphorylation. Therefore we conclude that part of the surface of CF1 subunit δ is exposed within the quaternary structure of the ATP-synthase complex of photosynthetically active thylakoids, but part of the surface of δ is shielded. Trypsination of isolated δ occurs at several sites, but this protease does not attack δ in situ, nor does aminopeptidase. Staphylococcus aureus protease V8 digests CF1 δ after isolation at residues Asp53, Glu61, Glu95 and Glu106, but has no access to these residues of δ in situ. Thus CF1 subunit δ seems to be shielded within the CF0CF1 complex to a large degree. Direct agglutination of thylakoids by anti δ serum 306 was weak and could be improved tenfold by a Coombs serum (goat anti rabbit gammaglobulin), whereas an anti β serum agglutinated directly. From this we conclude that δ is not accessible at the top of the enzyme; the exposed part is extending below the large subunits a and β and oriented towards the membrane.

scholarly journals Antimicrobial assessment of phage therapy using a porcine model of biofilm infection

2019 ◽  
Vol 557 ◽  
pp. 112-123 ◽  
Author(s):  
C. Milho ◽  
M. Andrade ◽  
D. Vilas Boas ◽  
D. Alves ◽  
S. Sillankorva
Keyword(s):  
Porcine Model ◽  
Phage Therapy ◽  
Biofilm Infection

Evaluation of phage therapy in the treatment of Staphylococcus aureus-induced mastitis in mice

2019 ◽  
Vol 65 (2) ◽  
pp. 339-351 ◽  
Author(s):  
Huijun Geng ◽  
Wei Zou ◽  
Meixia Zhang ◽  
Le Xu ◽  
Fanming Liu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phage Therapy
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