Enabling cassava starch hydrolysate for Pseudozyma tsukubaensis YWT 7-2 oil production using Ca(OH)2 treatment

2021 ◽  
Vol 55 (5) ◽  
Keyword(s):  
Oil Production ◽  
Cassava Starch ◽  
Pseudozyma Tsukubaensis ◽  
Starch Hydrolysate

scholarly journals Semi-continuous pilot-scale microbial oil production with Metschnikowia pulcherrima on starch hydrolysate

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Felix Abeln ◽  
Robert H. Hicks ◽  
Hadiza Auta ◽  
Mauro Moreno-Beltrán ◽  
Luca Longanesi ◽  
...  
Keyword(s):  
Oil Production ◽  
Pilot Scale ◽  
Microbial Oil ◽  
Metschnikowia Pulcherrima ◽  
Starch Hydrolysate

Fermentation of Cassava Starch Hydrolysate with Immobilised Cells ofSaccharomyces cerevisiae

1989 ◽  
Vol 41 (7) ◽  
pp. 271-275 ◽  
Author(s):  
K. Vijayagopal ◽  
C. Balagopalan
Keyword(s):  
Cassava Starch ◽  
Immobilised Cells ◽  
Starch Hydrolysate

scholarly journals Fouling Behaviour During Cross Flow Ultrafiltration of Cassava Starch Hydrolysate Using Polyacrylonitrile Membrane

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
I. N. Widiasa ◽  
I. G. Wenten
Keyword(s):  
Flow Velocity ◽  
Cross Flow ◽  
Cassava Starch ◽  
Alkaline Condition ◽  
Transmembrane Pressure ◽  
Permeate Flux ◽  
Promising Alternative ◽  
Glucose Syrup ◽  
Cross Flow Velocity ◽  
Starch Hydrolysate

Ultrafiltration (UF) is considered as a promising alternative to the traditional clarification techniques in the sugar industries. In this work, a hollow fiber UF membrane (polyacrylonitrile, MWCO 100 kDa) was used for clarification of cassava starch hydrolysate. The influence of various operating parameters, such as transmembrane pressure (TMP), cross flow velocity and pH hydrolysate on the membrane fouling was assessed. The results showed that TMP higher than 1.0 bar was not effective to improve flux. Increasing cross flow velocity was virtually effective to reduce permeate flux decline. The steady state flux, Jssincreased significantly when pH of the feed was adjusted to alkaline condition; however, this resulted in dark brown clarified glucose syrup. Operating at natural pH of 4.5, the membrane selectivity was close to 100%. Evaluation of hydraulic resistance indicated that concentration polarization and pore blocking were beyond approximately 50 and 40% of the total filtration resistance, respectively. Moreover, scanning electron microscopy showed that extensive fouling layer was deposited on the membrane surface. Finally, the developed cleaning procedure could restore membrane performance approximately 45% of its initial performance.

scholarly journals Fermentation and recovery of L-glutamic acid from cassava starch hydrolysate by ion-exchange resin column

1999 ◽  
Vol 30 (3) ◽  
pp. 258-264 ◽  
Author(s):  
K. Madhavan Nampoothiri ◽  
Ashok Pandey
Keyword(s):  
Ion Exchange ◽  
Glutamic Acid ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Cassava Starch ◽  
Manihot Esculenta Crantz ◽  
Batch Mode ◽  
Dextrose Equivalent ◽  
Shake Flasks ◽  
Starch Hydrolysate

Investigations were carried out with the aim of producing L-glutamic acid from Brevibacterium sp. by utilizing a locally available starchy substrate, cassava (Manihot esculenta Crantz). Initial studies were carried out in shake flasks, which showed that even though the yield was high with 85-90 DE (Dextrose Equivalent value), the maximum conversion yield (~34%) was obtained by using only partially digested starch hydrolysate, i.e. 45-50 DE. Fermentations were carried out in batch mode in a 5 L fermenter, using suitably diluted cassava starch hydrolysate, using a 85-90 DE value hydrolysate. Media supplemented with nutrients resulted in an accumulation of 21 g/L glutamic acid with a fairly high (66.3%) conversation yield of glucose to glutamic acid (based on glucose consumed and on 81.74% theoretical conversion rate). The bioreactor conditions most conducive for maximum production were pH 7.5, temperature 30°C and an agitation of 180 rpm. When fermentation was conducted in fed-batch mode by keeping the residual reducing sugar concentration at 5% w/v, 25.0 g/L of glutamate was obtained after 40 h fermentation (16% more the batch mode). Chromatographic separation by ion-exchange resin was used for the recovery and purification of glutamic acid. It was further crystallized and separated by making use of its low solubility at the isoelectric point (pH 3.2).

scholarly journals Carotenoid Production by Rhodosporidium paludigenum Using Cassava Starch Hydrolyzed by Bacillus subtilis as Substrate

2020 ◽  
Vol 2 (02) ◽  
pp. 36 ◽  
Author(s):  
Renna Eliana Warjoto ◽  
Felianti Felianti ◽  
Bibiana Widiyati Lay
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Animal Feed ◽  
Total Yield ◽  
Cassava Starch ◽  
Reducing Sugar ◽  
Sugar Concentration ◽  
Carotenoid Production ◽  
Starch Hydrolysate ◽  
Reducing Sugar Concentration

Carotenoids are natural pigments with colors ranging from yellow to red that are beneficial for food, cosmetics, and animal feed industries. These pigments can be found in fruits, vegetables, algae, and microorganisms. Among all microorganisms that have been known to produce carotenoids, Rhodosporidium paludigenum is still poorly investigated. Therefore, this study aimed to determine the potential of carotenoid production by R. paludigenum using cassava starch hydrolyzed by Bacillus subtilis as a substrate. The cassava starch for hydrolysis was divided into four concentrations, i.e., 2%, 4%, 6%, and 8% w/v. During the hydrolysis period, the amylase enzyme activity produced by B. subtilis was evaluated. The reducing sugar concentration was then examined to determine the optimum medium for carotenoid production. The highest amylase enzyme activity was produced on the second day in all cassava starch concentrations. However, the highest reducing sugar concentration was discovered in the 6% w/v cassava starch concentration. Thus, a batch submerged fermentation for carotenoid production by R. paludigenum was performed using the hydrolysate as the sole substrate. At the end of the fermentation, the total carotenoid was extracted, and the concentration was determined using spectrophotometry. The total yield of xanthophyll over biomass was higher than that of β-carotene. These findings elucidated the potency of cassava starch hydrolysate obtained from the starch hydrolyzed by B. subtilis, for carotenoid production by the red yeast R. paludigenum.

Poly-β-hydroxyalkanoates production from cassava starch hydrolysate by Cupriavidus sp. KKU38

2014 ◽  
Vol 65 ◽  
pp. 51-64 ◽  
Author(s):  
Natthaporn Poomipuk ◽  
Alissara Reungsang ◽  
Pensri Plangklang
Keyword(s):  
Cassava Starch ◽  
Starch Hydrolysate

Influence of composition of sugars in cassava starch hydrolysate on alcohol production

1986 ◽  
Vol 8 (6) ◽  
pp. 449-450 ◽  
Author(s):  
P. Prema ◽  
S. V. Ramakrishna ◽  
J. Madhusudhana Rao
Keyword(s):  
Cassava Starch ◽  
Alcohol Production ◽  
Starch Hydrolysate
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