Faculty Opinions recommendation of Duplication of the S-locus F-box gene is associated with breakdown of pollen function in an S-haplotype identified in a natural population of self-incompatible Petunia axillaris.

2005 ◽  
Author(s):  
Deborah Charlesworth
Keyword(s):  
Natural Population ◽  
S Locus ◽  
S Haplotype ◽  
Petunia Axillaris

scholarly journals Characterization of S haplotype in a new self-compatible Brassica rapa cultivar Dahuangyoucai

2013 ◽  
Vol 49 (No. 4) ◽  
pp. 157-163 ◽  
Author(s):  
X. Zhang ◽  
C. Ma ◽  
D. Yin ◽  
W. Zhu ◽  
C. Gao ◽  
...  
Keyword(s):  
Brassica Species ◽  
Functional Class ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility ◽  
Female Determinant ◽  
First Time ◽  
S Locus Glycoprotein ◽  
Signalling Cascade

The most important Brassica species, B. rapa, is naturally self-incompatible. Self-compatible mutants would be useful for dissecting the molecular mechanism of self-incompatibility (SI), a process that promotes outcrossing by recognizing and refusing self-pollens. The S haplotype in a new self-compatible B. rapa cultivar, Dahuangyoucai, was characterized for the first time in this study. Sequence analysis of the S-locus genes, SLG (S-locus glycoprotein), SRK (S-locus receptor kinase) and SCR (S-locus cysteine-rich protein) revealed that Dahuangyoucai contained S haplotype highly similar to S-f2, a non-functional class I S haplotype identified in another self-compatible B. rapa cultivar, Yellow Sarson. Mutations of MLPK (M-locus protein kinase) and non-transcription of the male determinant, SCR, were observed in this cultivar, which is similar to the situation reported in Yellow Sarson. With respect to the female determinant, SRK, no transcript was detected in Yellow Sarson but two fragments were detected in Dahuangyoucai. One fragment was highly similar to SRK-f2, but the other fragment was different from the signal factors previously identified in the SI reaction. The results suggest that Dahuangyoucai and Yellow Sarson have the same origin and a similar mechanism of self-compatibility, but diverge after mutations in SRK, SCR and MLPK. Further studying the self-compatibility of Dahuangyoucai might identify novel factors involved in the SI signalling cascade and provide new insights into the mechanisms of SI in Brassicaceae.

SELF-(IN)COMPATIBILITY IN PRUNUS ARMENIACA L.: ANALYSIS OF THE S-LOCUS STRUCTURE AND IDENTIFICATION OF S-HAPLOTYPE SPECIFIC S-RNASE

2006 ◽  
pp. 213-216
Author(s):  
S. Vilanova ◽  
C. Romero ◽  
L. Burgos ◽  
J. Martinez-Calvo ◽  
M. Vicente ◽  
...  
Keyword(s):  
Prunus Armeniaca ◽  
S Locus ◽  
S Haplotype ◽  
Prunus Armeniaca L

Nucleotide sequence analysis of S-locus genes to unify S haplotype nomenclature in radish

2018 ◽  
Vol 38 (9) ◽  
Author(s):  
Yurie Haseyama ◽  
Hiroyasu Kitashiba ◽  
Shunsuke Okamoto ◽  
Emiko Tonouchi ◽  
Koji Sakamoto ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
S Locus ◽  
S Haplotype

scholarly journals Cross-incompatibility and self-incompatibility: unrelated phenomena in wild and cultivated potatoes?

Botany ◽  
2018 ◽  
Vol 96 (1) ◽  
pp. 33-45 ◽  
Author(s):  
Juan Federico Maune ◽  
Elsa Lucila Camadro ◽  
Luis Ernesto Erazzú
Keyword(s):  
Germplasm Conservation ◽  
Solanum Chacoense ◽  
Molecular Evidence ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Family Level ◽  
Sib Families ◽  
Cultivated Potatoes ◽  
The Individual

Knowledge of internal hybridization barriers is relevant for germplasm conservation and utilization. The two pre-zygotic barriers are pollen–pistil self-incompatibility (SI) and cross-incompatibility (CI). To ascertain whether SI and CI were phenotypically related phenomena in potatoes, extensive intra- and interspecific, both intra- and interploidy breeding relationships were established, without previous assumptions on the compatibility behavior of the studied germplasm. Pollen–pistil relationships were analyzed at the individual genotype/accession/family level. In two seasons, 828 intra- and interspecific genotypic combinations were performed, using accessions of the wild potatoes Solanum chacoense Bitter (2n = 2x = 24), S. gourlayi Hawkes (2n = 2x = 24; 2n = 4x = 48), and S. spegazzinii Bitter (2n = 2x = 24), full-sibling (hereinafter “full-sib”) families (2n = 2x = 24) within/between the latter two diploids, and S. tuberosum L. (2n = 4x = 48) cultivars. Pollen–pistil incompatibility occurred in the upper first third of the style (I1/3) in all selfed diploids. In both the intra- and interspecific combinations, the most frequent relationship was compatibility, followed by I1/3, but incompatibility also occurred in the stigma and the style (middle third and bottom third). We observed segregation for these relationships in full-sib families, and unilateral and bilateral incompatibility in reciprocal crosses between functional SI genotypes. Cross-incompatibility in potatoes is, apparently, controlled by genes independent of the S-locus or its S-haplotype recognition region (although molecular evidence is needed to confirm it), with segregation even within accessions.

scholarly journals Intrahaplotype Polymorphism at the Brassica S Locus

Genetics ◽  
2001 ◽  
Vol 159 (2) ◽  
pp. 811-822
Author(s):  
Christine Miege ◽  
Véronique Ruffio-Châble ◽  
Mikkel H Schierup ◽  
Didier Cabrillac ◽  
Christian Dumas ◽  
...  
Keyword(s):  
Class Ii ◽  
The Self ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
S Haplotype

Abstract The S locus receptor kinase and the S locus glycoproteins are encoded by genes located at the S locus, which controls the self-incompatibility response in Brassica. In class II self-incompatibility haplotypes, S locus glycoproteins can be encoded by two different genes, SLGA and SLGB. In this study, we analyzed the sequences of these genes in several independently isolated plants, all of which carry the same S haplotype (S2). Two groups of S2 haplotypes could be distinguished depending on whether SRK was associated with SLGA or SLGB. Surprisingly, SRK alleles from the two groups could be distinguished at the sequence level, suggesting that recombination rarely occurs between haplotypes of the two groups. An analysis of the distribution of polymorphisms along the S domain of SRK showed that hypervariable domains I and II tend to be conserved within haplotypes but to be highly variable between haplotypes. This is consistent with these domains playing a role in the determination of haplotype specificity.

scholarly journals Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2186
Author(s):  
Zhiquan Liu ◽  
Bing Li ◽  
Yong Yang ◽  
Changbin Gao ◽  
Bin Yi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Locus ◽  
Recognition System ◽  
Inbred Lines ◽  
The Self ◽  
Self Incompatibility ◽  
S Locus ◽  
S Haplotype ◽  
Self Compatibility

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.

Breakdown of self-incompatibility in a natural population of Petunia axillaris caused by a modifier locus that suppresses the expression of an S-RNase gene

2003 ◽  
Vol 15 (5) ◽  
pp. 255-263 ◽  
Author(s):  
Tatsuya Tsukamoto ◽  
Toshio Ando ◽  
Hisashi Kokubun ◽  
Hitoshi Watanabe ◽  
Takahide Sato ◽  
...  
Keyword(s):  
Natural Population ◽  
Self Incompatibility ◽  
Modifier Locus ◽  
Petunia Axillaris

scholarly journals S-RNase proteins: functional studies of the 120kDa glycoprotein and SRNase oligomerization

2005 ◽  
Author(s):  
◽  
Charles Nathan Hancock
Keyword(s):  
Nicotiana Alata ◽  
Self Incompatibility ◽  
S Locus ◽  
Transmitting Tract ◽  
Functional Studies ◽  
S Haplotype ◽  
S Factor ◽  
Self Association ◽  
Incompatible Cross ◽  
F Box Protein

Flowering plants control fertilization through pollen-pistil interactions. Self-incompatibility (SI) is a well-studied pollen-pistil interaction that promotes cross-pollination. SI is controlled by a multi-haplotype locus called the S-locus. In Nicotiana alata, S-RNase is a product of the S-locus and regulates specificity in the pistil, while S-locus F-box protein (SLF) controls specificity in the pollen. The interaction between S-RNase and SLF determines whether the pollination is compatible or incompatible. In an incompatible cross, the ribonuclease activity of S-RNase inhibits pollen tube growth. Genetic experiments indicate that, in addition to S-RNase and SLF, non-S-factors are also required for SI. S-RNase binding proteins represent potential non-S-factors required for SI. Using affinity chromatography, we found that S-RNase selfassociates and three homologous stylar glycoproteins - the 120kDa glycoprotein (120K), N. alata pistil extensin-like protein III (NaPELP III), and N. alata transmitting tract specific glycoprotein (NaTTS) - bind directly to S-RNase. I studied the oligomerization of S-RNase in detail and found that self-association is dependent on S-haplotype and buffer conditions. I determined that the components of the S-RNase complex account for 30% of soluble pistil protein. 120K is the most likely candidate for a non-S-factor because it enters the cytoplasm of growing pollen tubes and shows polymorphism when SI and self-compatible Nicotiana species are compared. To test its role in SI, I suppressed 120K expression using RNAi. Suppressing 120K caused a breakdown of SI, confirming that it functions in SI.

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